Rational Design of Ratiometric Fluorescent Probe for Zn<sup>2+</sup> Imaging under Oxidative Stress in Cells
Zn<sup>2+</sup> is a vital ion for most of the physiological processes in the human body, and it usually has a mutual effect with oxidative stress that often occurs in pathological tissues. Detecting fluctuation of Zn<sup>2+</sup> level in cells undergoing oxidative stress co...
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| Format: | Article |
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MDPI AG
2022-11-01
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| Series: | Chemosensors |
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| Online Access: | https://www.mdpi.com/2227-9040/10/11/477 |
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| author | Yaheng Li Shankun Yao Hongbao Fang Weijiang He Yuncong Chen Zijian Guo |
| author_facet | Yaheng Li Shankun Yao Hongbao Fang Weijiang He Yuncong Chen Zijian Guo |
| author_sort | Yaheng Li |
| collection | DOAJ |
| description | Zn<sup>2+</sup> is a vital ion for most of the physiological processes in the human body, and it usually has a mutual effect with oxidative stress that often occurs in pathological tissues. Detecting fluctuation of Zn<sup>2+</sup> level in cells undergoing oxidative stress could be beneficial to understanding the relationship between them. Herein, a ratiometric fluorescent Zn<sup>2+</sup> probe was rationally designed. The wavelength corresponding to the maximum fluorescence intensity bathometrically shifted from 620 nm to 650 nm after coordinating with Zn<sup>2+</sup>. The intensity ratio of two fluorescence channels changed significantly in cells treated by oxidative stress inducers. It was shown from the results that the labile zinc level was generally elevated under oxidative stress stimulated by various inducers. |
| first_indexed | 2024-03-09T18:24:46Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2227-9040 |
| language | English |
| last_indexed | 2024-03-09T18:24:46Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Chemosensors |
| spelling | doaj.art-be719ec925f140e4afbe5929798642b72023-11-24T07:59:49ZengMDPI AGChemosensors2227-90402022-11-01101147710.3390/chemosensors10110477Rational Design of Ratiometric Fluorescent Probe for Zn<sup>2+</sup> Imaging under Oxidative Stress in CellsYaheng Li0Shankun Yao1Hongbao Fang2Weijiang He3Yuncong Chen4Zijian Guo5State Key Laboratory of Coordination Chemistry Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, ChinaState Key Laboratory of Coordination Chemistry Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, ChinaState Key Laboratory of Coordination Chemistry Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, ChinaState Key Laboratory of Coordination Chemistry Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, ChinaState Key Laboratory of Coordination Chemistry Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, ChinaState Key Laboratory of Coordination Chemistry Coordination Chemistry, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, ChinaZn<sup>2+</sup> is a vital ion for most of the physiological processes in the human body, and it usually has a mutual effect with oxidative stress that often occurs in pathological tissues. Detecting fluctuation of Zn<sup>2+</sup> level in cells undergoing oxidative stress could be beneficial to understanding the relationship between them. Herein, a ratiometric fluorescent Zn<sup>2+</sup> probe was rationally designed. The wavelength corresponding to the maximum fluorescence intensity bathometrically shifted from 620 nm to 650 nm after coordinating with Zn<sup>2+</sup>. The intensity ratio of two fluorescence channels changed significantly in cells treated by oxidative stress inducers. It was shown from the results that the labile zinc level was generally elevated under oxidative stress stimulated by various inducers.https://www.mdpi.com/2227-9040/10/11/477Zn<sup>2+</sup>oxidative stressfluorescence imagingBODIPYratiometric probe |
| spellingShingle | Yaheng Li Shankun Yao Hongbao Fang Weijiang He Yuncong Chen Zijian Guo Rational Design of Ratiometric Fluorescent Probe for Zn<sup>2+</sup> Imaging under Oxidative Stress in Cells Chemosensors Zn<sup>2+</sup> oxidative stress fluorescence imaging BODIPY ratiometric probe |
| title | Rational Design of Ratiometric Fluorescent Probe for Zn<sup>2+</sup> Imaging under Oxidative Stress in Cells |
| title_full | Rational Design of Ratiometric Fluorescent Probe for Zn<sup>2+</sup> Imaging under Oxidative Stress in Cells |
| title_fullStr | Rational Design of Ratiometric Fluorescent Probe for Zn<sup>2+</sup> Imaging under Oxidative Stress in Cells |
| title_full_unstemmed | Rational Design of Ratiometric Fluorescent Probe for Zn<sup>2+</sup> Imaging under Oxidative Stress in Cells |
| title_short | Rational Design of Ratiometric Fluorescent Probe for Zn<sup>2+</sup> Imaging under Oxidative Stress in Cells |
| title_sort | rational design of ratiometric fluorescent probe for zn sup 2 sup imaging under oxidative stress in cells |
| topic | Zn<sup>2+</sup> oxidative stress fluorescence imaging BODIPY ratiometric probe |
| url | https://www.mdpi.com/2227-9040/10/11/477 |
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