Abalone visceral peptides containing Cys and Tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damage
The in vitro antioxidation and cytoprotection of abalone visceral peptides against oxidative damage were investigated. Results show that the DPPH· scavenging activities of the 16 chemically synthesized peptides were significantly and positively correlated with their reducing power. Their scavenging...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-03-01
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| Series: | Food Chemistry: X |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S259015752300024X |
| _version_ | 1797868313440681984 |
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| author | Jing Liu Guohong Wu Jiahong Yang Chuanbo He Hejian Xiong Ying Ma |
| author_facet | Jing Liu Guohong Wu Jiahong Yang Chuanbo He Hejian Xiong Ying Ma |
| author_sort | Jing Liu |
| collection | DOAJ |
| description | The in vitro antioxidation and cytoprotection of abalone visceral peptides against oxidative damage were investigated. Results show that the DPPH· scavenging activities of the 16 chemically synthesized peptides were significantly and positively correlated with their reducing power. Their scavenging activities against ABTS·+ were positively correlated with their ability to inhibit linoleic acid oxidation. Only Cys containing peptides exhibited good DPPH· scavenging activity, while only Tyr containing peptides showed significant ABTS·+ scavenging activity. In the cytoprotection assay, all four representative peptides significantly increased the viability of H2O2-damaged LO2 cells and the activities of GSH-Px, CAT, and SOD, and all decreased MDA levels and LDH leakage, in which the Cys-containing peptides were more effective at increasing the activities of antioxidant enzymes, while the Tyr-containing peptides were more effective at decreasing MDA levels and LDH leakage. Abalone visceral peptides containing both Cys and Tyr exhibit strong in vitro and cellular antioxidation. |
| first_indexed | 2024-04-09T23:53:54Z |
| format | Article |
| id | doaj.art-be74137f31d344b289840bcd42efe606 |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-04-09T23:53:54Z |
| publishDate | 2023-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-be74137f31d344b289840bcd42efe6062023-03-17T04:34:21ZengElsevierFood Chemistry: X2590-15752023-03-0117100582Abalone visceral peptides containing Cys and Tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damageJing Liu0Guohong Wu1Jiahong Yang2Chuanbo He3Hejian Xiong4Ying Ma5College of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen 361021, ChinaCollege of Ocean Food and Biological Engineering, Fujian Provincial Engineering Technology Research Center of Marine Functional Food, Jimei University, Xiamen 361021, China; Corresponding authors.Fisheries College of Jimei University, Xiamen 361021, China; Corresponding authors.The in vitro antioxidation and cytoprotection of abalone visceral peptides against oxidative damage were investigated. Results show that the DPPH· scavenging activities of the 16 chemically synthesized peptides were significantly and positively correlated with their reducing power. Their scavenging activities against ABTS·+ were positively correlated with their ability to inhibit linoleic acid oxidation. Only Cys containing peptides exhibited good DPPH· scavenging activity, while only Tyr containing peptides showed significant ABTS·+ scavenging activity. In the cytoprotection assay, all four representative peptides significantly increased the viability of H2O2-damaged LO2 cells and the activities of GSH-Px, CAT, and SOD, and all decreased MDA levels and LDH leakage, in which the Cys-containing peptides were more effective at increasing the activities of antioxidant enzymes, while the Tyr-containing peptides were more effective at decreasing MDA levels and LDH leakage. Abalone visceral peptides containing both Cys and Tyr exhibit strong in vitro and cellular antioxidation.http://www.sciencedirect.com/science/article/pii/S259015752300024XAbalone visceraAntioxidant peptideDPPH radical scavenging activityABTS radical scavenging activityCell damageStructure-activity relationship |
| spellingShingle | Jing Liu Guohong Wu Jiahong Yang Chuanbo He Hejian Xiong Ying Ma Abalone visceral peptides containing Cys and Tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damage Food Chemistry: X Abalone viscera Antioxidant peptide DPPH radical scavenging activity ABTS radical scavenging activity Cell damage Structure-activity relationship |
| title | Abalone visceral peptides containing Cys and Tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damage |
| title_full | Abalone visceral peptides containing Cys and Tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damage |
| title_fullStr | Abalone visceral peptides containing Cys and Tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damage |
| title_full_unstemmed | Abalone visceral peptides containing Cys and Tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damage |
| title_short | Abalone visceral peptides containing Cys and Tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damage |
| title_sort | abalone visceral peptides containing cys and tyr exhibit strong in vitro antioxidant activity and cytoprotective effects against oxidative damage |
| topic | Abalone viscera Antioxidant peptide DPPH radical scavenging activity ABTS radical scavenging activity Cell damage Structure-activity relationship |
| url | http://www.sciencedirect.com/science/article/pii/S259015752300024X |
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