Altered sympathovagal balance and pain hypersensitivity in TNBS-induced colitis
Introduction : Pain hypersensitivity, abnormal motility and autonomic dysfunction contribute to functional symptoms of inflammatory bowel disease (IBD). Material and methods : The aim of this study was to assess: nociceptive thresholds for mechanical allodynia (MA) and thermal hyperalgesia (TH), i...
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| Format: | Article |
| Language: | English |
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Termedia Publishing House
2016-12-01
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| Series: | Archives of Medical Science |
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| Online Access: | https://www.termedia.pl/Altered-sympathovagal-balance-and-pain-hypersensitivity-in-TNBS-induced-colitis,19,26034,1,1.html |
| _version_ | 1819102269115727872 |
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| author | Katarzyna Ciesielczyk Agata Furgała Łukasz Dobrek Kajetan Juszczak Piotr Thor |
| author_facet | Katarzyna Ciesielczyk Agata Furgała Łukasz Dobrek Kajetan Juszczak Piotr Thor |
| author_sort | Katarzyna Ciesielczyk |
| collection | DOAJ |
| description | Introduction : Pain hypersensitivity, abnormal motility and autonomic dysfunction contribute to functional symptoms of inflammatory bowel disease (IBD).
Material and methods : The aim of this study was to assess: nociceptive thresholds for mechanical allodynia (MA) and thermal hyperalgesia (TH), intestinal motility (distal colonic transit and emptying), and cardiac autonomic neuropathy (indices of heart rate variability – HRV) in male Wistar rats with experimental trinitrobenzene sulfonic acid (TNBS) induced colitis. To identify a potential vagal contribution the bilateral subdiaphragmatic vagotomy (SDV) was performed.
Results: Experimental colitis resulted in a significant decrease in pain threshold (MA 23.60 ±2.12, p < 0.001, TH 8.51 ±1.49, p < 0.001), reduced expulsion time (6.2 ±3.5, p < 0,01) and increase in the sympathetic autonomic activity (LFnu 32.54 ±21.16, p < 0.03). The animals with diminished vagal integrity presented with reduced gastrointestinal motility (39.8 ±25.1, p < 0.01) and a decrease in the parasympathetic high-frequency domain of HRV (HFnu 55.37 ±22.80, p < 0.002). The vagotomized rats with colitis showed the strongest nociceptive response (MA 22.46 ±3.02, p < 0.004; TH 7.99 ±1.12, p < 0.003) as well as significant changes in sympatho-vagal balance on HRV testing (LFnu 28.25 ±14.66, p < 0.04; HFnu 71.34 ±14.55, p < 0.04).
Conclusions : The relationship between the cardiovascular and gastrointestinal system is modulated by neural, hormonal and inflammatory factors. This leads to dysregulation of the brain-gut interactions in the course of IBD. Sensitization and visceral-somatic convergence trigger pain hypersensitivity and autonomic sympathovagal imbalance. While integral vagal innervation impacts analgesic mechanisms via modulation of the immune response, SDV raises sympathetic activity and induces excessive hyperalgesia. |
| first_indexed | 2024-12-22T01:31:52Z |
| format | Article |
| id | doaj.art-be756aaa95674459a83ddd570f8843ff |
| institution | Directory Open Access Journal |
| issn | 1734-1922 1896-9151 |
| language | English |
| last_indexed | 2024-12-22T01:31:52Z |
| publishDate | 2016-12-01 |
| publisher | Termedia Publishing House |
| record_format | Article |
| series | Archives of Medical Science |
| spelling | doaj.art-be756aaa95674459a83ddd570f8843ff2022-12-21T18:43:28ZengTermedia Publishing HouseArchives of Medical Science1734-19221896-91512016-12-0113124625510.5114/aoms.2015.5514726034Altered sympathovagal balance and pain hypersensitivity in TNBS-induced colitisKatarzyna CiesielczykAgata FurgałaŁukasz DobrekKajetan JuszczakPiotr ThorIntroduction : Pain hypersensitivity, abnormal motility and autonomic dysfunction contribute to functional symptoms of inflammatory bowel disease (IBD). Material and methods : The aim of this study was to assess: nociceptive thresholds for mechanical allodynia (MA) and thermal hyperalgesia (TH), intestinal motility (distal colonic transit and emptying), and cardiac autonomic neuropathy (indices of heart rate variability – HRV) in male Wistar rats with experimental trinitrobenzene sulfonic acid (TNBS) induced colitis. To identify a potential vagal contribution the bilateral subdiaphragmatic vagotomy (SDV) was performed. Results: Experimental colitis resulted in a significant decrease in pain threshold (MA 23.60 ±2.12, p < 0.001, TH 8.51 ±1.49, p < 0.001), reduced expulsion time (6.2 ±3.5, p < 0,01) and increase in the sympathetic autonomic activity (LFnu 32.54 ±21.16, p < 0.03). The animals with diminished vagal integrity presented with reduced gastrointestinal motility (39.8 ±25.1, p < 0.01) and a decrease in the parasympathetic high-frequency domain of HRV (HFnu 55.37 ±22.80, p < 0.002). The vagotomized rats with colitis showed the strongest nociceptive response (MA 22.46 ±3.02, p < 0.004; TH 7.99 ±1.12, p < 0.003) as well as significant changes in sympatho-vagal balance on HRV testing (LFnu 28.25 ±14.66, p < 0.04; HFnu 71.34 ±14.55, p < 0.04). Conclusions : The relationship between the cardiovascular and gastrointestinal system is modulated by neural, hormonal and inflammatory factors. This leads to dysregulation of the brain-gut interactions in the course of IBD. Sensitization and visceral-somatic convergence trigger pain hypersensitivity and autonomic sympathovagal imbalance. While integral vagal innervation impacts analgesic mechanisms via modulation of the immune response, SDV raises sympathetic activity and induces excessive hyperalgesia.https://www.termedia.pl/Altered-sympathovagal-balance-and-pain-hypersensitivity-in-TNBS-induced-colitis,19,26034,1,1.htmlinflammatory bowel disease colitis pain hypersensitivity heart rate variability |
| spellingShingle | Katarzyna Ciesielczyk Agata Furgała Łukasz Dobrek Kajetan Juszczak Piotr Thor Altered sympathovagal balance and pain hypersensitivity in TNBS-induced colitis Archives of Medical Science inflammatory bowel disease colitis pain hypersensitivity heart rate variability |
| title | Altered sympathovagal balance and pain hypersensitivity in TNBS-induced colitis |
| title_full | Altered sympathovagal balance and pain hypersensitivity in TNBS-induced colitis |
| title_fullStr | Altered sympathovagal balance and pain hypersensitivity in TNBS-induced colitis |
| title_full_unstemmed | Altered sympathovagal balance and pain hypersensitivity in TNBS-induced colitis |
| title_short | Altered sympathovagal balance and pain hypersensitivity in TNBS-induced colitis |
| title_sort | altered sympathovagal balance and pain hypersensitivity in tnbs induced colitis |
| topic | inflammatory bowel disease colitis pain hypersensitivity heart rate variability |
| url | https://www.termedia.pl/Altered-sympathovagal-balance-and-pain-hypersensitivity-in-TNBS-induced-colitis,19,26034,1,1.html |
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