Measuring compound eye optics with microscope and microCT images
Abstract With a great variety of shapes and sizes, compound eye morphologies give insight into visual ecology, development, and evolution, and inspire novel engineering. In contrast to our own camera-type eyes, compound eyes reveal their resolution, sensitivity, and field of view externally, provide...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-03-01
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| Series: | Communications Biology |
| Online Access: | https://doi.org/10.1038/s42003-023-04575-x |
| _version_ | 1797789657896845312 |
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| author | John Paul Currea Yash Sondhi Akito Y. Kawahara Jamie Theobald |
| author_facet | John Paul Currea Yash Sondhi Akito Y. Kawahara Jamie Theobald |
| author_sort | John Paul Currea |
| collection | DOAJ |
| description | Abstract With a great variety of shapes and sizes, compound eye morphologies give insight into visual ecology, development, and evolution, and inspire novel engineering. In contrast to our own camera-type eyes, compound eyes reveal their resolution, sensitivity, and field of view externally, provided they have spherical curvature and orthogonal ommatidia. Non-spherical compound eyes with skewed ommatidia require measuring internal structures, such as with MicroCT (µCT). Thus far, there is no efficient tool to characterize compound eye optics, from either 2D or 3D data, automatically. Here we present two open-source programs: (1) the ommatidia detecting algorithm (ODA), which measures ommatidia count and diameter in 2D images, and (2) a µCT pipeline (ODA-3D), which calculates anatomical acuity, sensitivity, and field of view across the eye by applying the ODA to 3D data. We validate these algorithms on images, images of replicas, and µCT eye scans from ants, fruit flies, moths, and a bee. |
| first_indexed | 2024-03-13T01:53:47Z |
| format | Article |
| id | doaj.art-be781396a1c1425dbe11fa60ce021f13 |
| institution | Directory Open Access Journal |
| issn | 2399-3642 |
| language | English |
| last_indexed | 2024-03-13T01:53:47Z |
| publishDate | 2023-03-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Communications Biology |
| spelling | doaj.art-be781396a1c1425dbe11fa60ce021f132023-07-02T11:23:20ZengNature PortfolioCommunications Biology2399-36422023-03-016111210.1038/s42003-023-04575-xMeasuring compound eye optics with microscope and microCT imagesJohn Paul Currea0Yash Sondhi1Akito Y. Kawahara2Jamie Theobald3Integrative Biology and Physiology, UCLADepartment of Biological Sciences, Florida International UniversityMcGuire Center for Lepidoptera and Biodiversity, Florida Museum of Natural History, University of FloridaDepartment of Biological Sciences, Florida International UniversityAbstract With a great variety of shapes and sizes, compound eye morphologies give insight into visual ecology, development, and evolution, and inspire novel engineering. In contrast to our own camera-type eyes, compound eyes reveal their resolution, sensitivity, and field of view externally, provided they have spherical curvature and orthogonal ommatidia. Non-spherical compound eyes with skewed ommatidia require measuring internal structures, such as with MicroCT (µCT). Thus far, there is no efficient tool to characterize compound eye optics, from either 2D or 3D data, automatically. Here we present two open-source programs: (1) the ommatidia detecting algorithm (ODA), which measures ommatidia count and diameter in 2D images, and (2) a µCT pipeline (ODA-3D), which calculates anatomical acuity, sensitivity, and field of view across the eye by applying the ODA to 3D data. We validate these algorithms on images, images of replicas, and µCT eye scans from ants, fruit flies, moths, and a bee.https://doi.org/10.1038/s42003-023-04575-x |
| spellingShingle | John Paul Currea Yash Sondhi Akito Y. Kawahara Jamie Theobald Measuring compound eye optics with microscope and microCT images Communications Biology |
| title | Measuring compound eye optics with microscope and microCT images |
| title_full | Measuring compound eye optics with microscope and microCT images |
| title_fullStr | Measuring compound eye optics with microscope and microCT images |
| title_full_unstemmed | Measuring compound eye optics with microscope and microCT images |
| title_short | Measuring compound eye optics with microscope and microCT images |
| title_sort | measuring compound eye optics with microscope and microct images |
| url | https://doi.org/10.1038/s42003-023-04575-x |
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