Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with Bacteriophages
The capacity to load bacteriophages into electrospun nanofibers of two representative biocompatible polymers has been evaluated, paying special attention to the possibility of preserving their antibacterial activity. Specifically, the work involves the following steps: (a) Evaluation of the effect o...
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MDPI AG
2018-05-01
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| Online Access: | http://www.mdpi.com/2079-6439/6/2/33 |
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| author | Angélica Díaz Luis J. del Valle Noel Rodrigo María Teresa Casas George Chumburidze Ramaz Katsarava Jordi Puiggalí |
| author_facet | Angélica Díaz Luis J. del Valle Noel Rodrigo María Teresa Casas George Chumburidze Ramaz Katsarava Jordi Puiggalí |
| author_sort | Angélica Díaz |
| collection | DOAJ |
| description | The capacity to load bacteriophages into electrospun nanofibers of two representative biocompatible polymers has been evaluated, paying special attention to the possibility of preserving their antibacterial activity. Specifically, the work involves the following steps: (a) Evaluation of the effect of the applied electrical field on the phage activity; (b) evaluation of the activity when a lyophilization process could be avoided by using water soluble polymers (e.g., poly(ethylene glycol); (c) evaluation of the activity when dissolution of the polymer requires an organic solvent and lyophilization is theoretically necessary. In this case, a poly(ester urea) (PEU) derived from the natural L-leucine amino acid has been considered. Adsorption of commercial bacteriophage preparations into calcium carbonate particles was demonstrated to be a promising methodology to avoid lyophilization and keep the initial bactericide activity at a maximum. Phagestaph and Fersis bacteriophage commercial preparations have been selected for this study due to their specific activity against Staphylococci (e.g., S. aureus) and Streptococci (e.g., S. pyogenes) bacteria. Adhesion and proliferation assays using epithelial cells demonstrated the biocompatibility of both unloaded and bacteriophage-loaded PEU scaffolds, although some slight differences were observed depending on the type of bacteriophage and the selected preparation methodology. |
| first_indexed | 2024-12-10T08:19:13Z |
| format | Article |
| id | doaj.art-be7acfe42c1045cf924979895397f9c5 |
| institution | Directory Open Access Journal |
| issn | 2079-6439 |
| language | English |
| last_indexed | 2024-12-10T08:19:13Z |
| publishDate | 2018-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fibers |
| spelling | doaj.art-be7acfe42c1045cf924979895397f9c52022-12-22T01:56:23ZengMDPI AGFibers2079-64392018-05-01623310.3390/fib6020033fib6020033Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with BacteriophagesAngélica Díaz0Luis J. del Valle1Noel Rodrigo2María Teresa Casas3George Chumburidze4Ramaz Katsarava5Jordi Puiggalí6Departament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, SpainDepartament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, SpainDepartament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, SpainDepartament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, SpainCenter for Medical Biotechnology & Bioengineering, Georgian Technical University, 77 Kostava str., Tbilisi 0175, GeorgiaCenter for Medical Biotechnology & Bioengineering, Georgian Technical University, 77 Kostava str., Tbilisi 0175, GeorgiaDepartament d’Enginyeria Química, EEBE, Universitat Politècnica de Catalunya, Edifici I.2, C/Eduard Maristany, 10-14, 08019 Barcelona, SpainThe capacity to load bacteriophages into electrospun nanofibers of two representative biocompatible polymers has been evaluated, paying special attention to the possibility of preserving their antibacterial activity. Specifically, the work involves the following steps: (a) Evaluation of the effect of the applied electrical field on the phage activity; (b) evaluation of the activity when a lyophilization process could be avoided by using water soluble polymers (e.g., poly(ethylene glycol); (c) evaluation of the activity when dissolution of the polymer requires an organic solvent and lyophilization is theoretically necessary. In this case, a poly(ester urea) (PEU) derived from the natural L-leucine amino acid has been considered. Adsorption of commercial bacteriophage preparations into calcium carbonate particles was demonstrated to be a promising methodology to avoid lyophilization and keep the initial bactericide activity at a maximum. Phagestaph and Fersis bacteriophage commercial preparations have been selected for this study due to their specific activity against Staphylococci (e.g., S. aureus) and Streptococci (e.g., S. pyogenes) bacteria. Adhesion and proliferation assays using epithelial cells demonstrated the biocompatibility of both unloaded and bacteriophage-loaded PEU scaffolds, although some slight differences were observed depending on the type of bacteriophage and the selected preparation methodology.http://www.mdpi.com/2079-6439/6/2/33bacteriophageselectrospinningscaffoldspoly(ester urea)sbactericidesbiocompatibility |
| spellingShingle | Angélica Díaz Luis J. del Valle Noel Rodrigo María Teresa Casas George Chumburidze Ramaz Katsarava Jordi Puiggalí Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with Bacteriophages Fibers bacteriophages electrospinning scaffolds poly(ester urea)s bactericides biocompatibility |
| title | Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with Bacteriophages |
| title_full | Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with Bacteriophages |
| title_fullStr | Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with Bacteriophages |
| title_full_unstemmed | Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with Bacteriophages |
| title_short | Antimicrobial Activity of Poly(ester urea) Electrospun Fibers Loaded with Bacteriophages |
| title_sort | antimicrobial activity of poly ester urea electrospun fibers loaded with bacteriophages |
| topic | bacteriophages electrospinning scaffolds poly(ester urea)s bactericides biocompatibility |
| url | http://www.mdpi.com/2079-6439/6/2/33 |
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