<i>VdPT1</i> Encoding a Neutral Trehalase of <i>Verticillium dahliae</i> Is Required for Growth and Virulence of the Pathogen
<i>Verticillum dahliae</i> is a soil-borne phytopathogenic fungus causing destructive Verticillium wilt disease. We previously found a trehalase-encoding gene (<i>VdPT1</i>) in <i>V. dahliae</i> being significantly up-regulated after sensing root exudates from a s...
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/25/1/294 |
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| author | Lihua Chen Xiaohu Ma Tiange Sun Qian-Hao Zhu Hongjie Feng Yongtai Li Feng Liu Xinyu Zhang Jie Sun Yanjun Li |
| author_facet | Lihua Chen Xiaohu Ma Tiange Sun Qian-Hao Zhu Hongjie Feng Yongtai Li Feng Liu Xinyu Zhang Jie Sun Yanjun Li |
| author_sort | Lihua Chen |
| collection | DOAJ |
| description | <i>Verticillum dahliae</i> is a soil-borne phytopathogenic fungus causing destructive Verticillium wilt disease. We previously found a trehalase-encoding gene (<i>VdPT1</i>) in <i>V. dahliae</i> being significantly up-regulated after sensing root exudates from a susceptible cotton variety. In this study, we characterized the function of <i>VdPT1</i> in the growth and virulence of <i>V. dahliae</i> using its deletion-mutant strains. The <i>VdPT1</i> deletion mutants (<i>ΔVdPT1</i>) displayed slow colony expansion and mycelial growth, reduced conidial production and germination rate, and decreased mycelial penetration ability and virulence on cotton, but exhibited enhanced stress resistance, suggesting that <i>VdPT1</i> is involved in the growth, pathogenesis, and stress resistance of <i>V. dahliae</i>. Host-induced silencing of <i>VdPT1</i> in cotton reduced fungal biomass and enhanced cotton resistance against <i>V. dahliae</i>. Comparative transcriptome analysis between wild-type and mutant identified 1480 up-regulated and 1650 down-regulated genes in the <i>ΔVdPT1</i> strain. Several down-regulated genes encode plant cell wall-degrading enzymes required for full virulence of <i>V. dahliae</i> to cotton, and down-regulated genes related to carbon metabolism, DNA replication, and amino acid biosynthesis seemed to be responsible for the decreased growth of the <i>ΔVdPT1</i> strain. In contrast, up-regulation of several genes related to glycerophospholipid metabolism in the <i>ΔVdPT1</i> strain enhanced the stress resistance of the mutated strain. |
| first_indexed | 2024-03-08T15:05:39Z |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-08T15:05:39Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-be7c1189962443c2bbeb0447015996ce2024-01-10T14:58:54ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-12-0125129410.3390/ijms25010294<i>VdPT1</i> Encoding a Neutral Trehalase of <i>Verticillium dahliae</i> Is Required for Growth and Virulence of the PathogenLihua Chen0Xiaohu Ma1Tiange Sun2Qian-Hao Zhu3Hongjie Feng4Yongtai Li5Feng Liu6Xinyu Zhang7Jie Sun8Yanjun Li9The Key Laboratory of Oasis Eco-Agriculture, Agriculture College, Shihezi University, Shihezi 832000, ChinaThe Key Laboratory of Oasis Eco-Agriculture, Agriculture College, Shihezi University, Shihezi 832000, ChinaThe Key Laboratory of Oasis Eco-Agriculture, Agriculture College, Shihezi University, Shihezi 832000, ChinaCSIRO Agriculture and Food, GPO Box 1700, Canberra 2601, AustraliaState Key Laboratory of Cotton Biology, Institute of Cotton Research of Chinese Academy of Agricultural Sciences, Anyang 455000, ChinaThe Key Laboratory of Oasis Eco-Agriculture, Agriculture College, Shihezi University, Shihezi 832000, ChinaThe Key Laboratory of Oasis Eco-Agriculture, Agriculture College, Shihezi University, Shihezi 832000, ChinaThe Key Laboratory of Oasis Eco-Agriculture, Agriculture College, Shihezi University, Shihezi 832000, ChinaThe Key Laboratory of Oasis Eco-Agriculture, Agriculture College, Shihezi University, Shihezi 832000, ChinaThe Key Laboratory of Oasis Eco-Agriculture, Agriculture College, Shihezi University, Shihezi 832000, China<i>Verticillum dahliae</i> is a soil-borne phytopathogenic fungus causing destructive Verticillium wilt disease. We previously found a trehalase-encoding gene (<i>VdPT1</i>) in <i>V. dahliae</i> being significantly up-regulated after sensing root exudates from a susceptible cotton variety. In this study, we characterized the function of <i>VdPT1</i> in the growth and virulence of <i>V. dahliae</i> using its deletion-mutant strains. The <i>VdPT1</i> deletion mutants (<i>ΔVdPT1</i>) displayed slow colony expansion and mycelial growth, reduced conidial production and germination rate, and decreased mycelial penetration ability and virulence on cotton, but exhibited enhanced stress resistance, suggesting that <i>VdPT1</i> is involved in the growth, pathogenesis, and stress resistance of <i>V. dahliae</i>. Host-induced silencing of <i>VdPT1</i> in cotton reduced fungal biomass and enhanced cotton resistance against <i>V. dahliae</i>. Comparative transcriptome analysis between wild-type and mutant identified 1480 up-regulated and 1650 down-regulated genes in the <i>ΔVdPT1</i> strain. Several down-regulated genes encode plant cell wall-degrading enzymes required for full virulence of <i>V. dahliae</i> to cotton, and down-regulated genes related to carbon metabolism, DNA replication, and amino acid biosynthesis seemed to be responsible for the decreased growth of the <i>ΔVdPT1</i> strain. In contrast, up-regulation of several genes related to glycerophospholipid metabolism in the <i>ΔVdPT1</i> strain enhanced the stress resistance of the mutated strain.https://www.mdpi.com/1422-0067/25/1/294cottonRNA-sequencingRNA-seqtrehalase<i>Verticillium dahliae</i> |
| spellingShingle | Lihua Chen Xiaohu Ma Tiange Sun Qian-Hao Zhu Hongjie Feng Yongtai Li Feng Liu Xinyu Zhang Jie Sun Yanjun Li <i>VdPT1</i> Encoding a Neutral Trehalase of <i>Verticillium dahliae</i> Is Required for Growth and Virulence of the Pathogen International Journal of Molecular Sciences cotton RNA-sequencing RNA-seq trehalase <i>Verticillium dahliae</i> |
| title | <i>VdPT1</i> Encoding a Neutral Trehalase of <i>Verticillium dahliae</i> Is Required for Growth and Virulence of the Pathogen |
| title_full | <i>VdPT1</i> Encoding a Neutral Trehalase of <i>Verticillium dahliae</i> Is Required for Growth and Virulence of the Pathogen |
| title_fullStr | <i>VdPT1</i> Encoding a Neutral Trehalase of <i>Verticillium dahliae</i> Is Required for Growth and Virulence of the Pathogen |
| title_full_unstemmed | <i>VdPT1</i> Encoding a Neutral Trehalase of <i>Verticillium dahliae</i> Is Required for Growth and Virulence of the Pathogen |
| title_short | <i>VdPT1</i> Encoding a Neutral Trehalase of <i>Verticillium dahliae</i> Is Required for Growth and Virulence of the Pathogen |
| title_sort | i vdpt1 i encoding a neutral trehalase of i verticillium dahliae i is required for growth and virulence of the pathogen |
| topic | cotton RNA-sequencing RNA-seq trehalase <i>Verticillium dahliae</i> |
| url | https://www.mdpi.com/1422-0067/25/1/294 |
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