Protective effect of sodium selenite against the genotoxicity of aflatoxin B1 in human whole blood cultures
This study was designed to investigate the effects of selenium and aflatoxin on human whole blood cultures (WBC) in relation to induction of sister-chromatid exchange (SCE). The results showed that the frequency of SCEs in peripheral lymphocytes was significantly increased by the direct-acting mutag...
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| Format: | Article |
| Language: | English |
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Instituto de Tecnologia do Paraná (Tecpar)
2006-05-01
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| Series: | Brazilian Archives of Biology and Technology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400006 |
| _version_ | 1819315536883875840 |
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| author | Fatime Geyikoglu Hasan Türkez |
| author_facet | Fatime Geyikoglu Hasan Türkez |
| author_sort | Fatime Geyikoglu |
| collection | DOAJ |
| description | This study was designed to investigate the effects of selenium and aflatoxin on human whole blood cultures (WBC) in relation to induction of sister-chromatid exchange (SCE). The results showed that the frequency of SCEs in peripheral lymphocytes was significantly increased by the direct-acting mutagen AFB1 (at doses 5 and 10 µM except for 1µM) compared to controls. When sodium selenite (Na2SeO3) was added alone at a molar ratio of 5x10-7 and 1x10-6, cells did not show significant increase in SCE frequency. Whereas, SCE rates induced by the various AFB1 concentrations could be significantly reduced by the presence of Na2SeO3 in a clear dose-related manner. These results indicated that selenite and AFB1 mutually antagonized their ability to cause DNA damage leading to the formation of SCEs. However, selenium didn't completely inhibit induction of SCEs by AFB1 compared to controls. This is first report describing, the protective ability of selenium againist AFB1 genotoxicity on human WBC. |
| first_indexed | 2024-12-24T10:01:40Z |
| format | Article |
| id | doaj.art-be7c6380b2544b07b0af65ce2adf048b |
| institution | Directory Open Access Journal |
| issn | 1516-8913 1678-4324 |
| language | English |
| last_indexed | 2024-12-24T10:01:40Z |
| publishDate | 2006-05-01 |
| publisher | Instituto de Tecnologia do Paraná (Tecpar) |
| record_format | Article |
| series | Brazilian Archives of Biology and Technology |
| spelling | doaj.art-be7c6380b2544b07b0af65ce2adf048b2022-12-21T17:01:01ZengInstituto de Tecnologia do Paraná (Tecpar)Brazilian Archives of Biology and Technology1516-89131678-43242006-05-0149339339810.1590/S1516-89132006000400006Protective effect of sodium selenite against the genotoxicity of aflatoxin B1 in human whole blood culturesFatime GeyikogluHasan TürkezThis study was designed to investigate the effects of selenium and aflatoxin on human whole blood cultures (WBC) in relation to induction of sister-chromatid exchange (SCE). The results showed that the frequency of SCEs in peripheral lymphocytes was significantly increased by the direct-acting mutagen AFB1 (at doses 5 and 10 µM except for 1µM) compared to controls. When sodium selenite (Na2SeO3) was added alone at a molar ratio of 5x10-7 and 1x10-6, cells did not show significant increase in SCE frequency. Whereas, SCE rates induced by the various AFB1 concentrations could be significantly reduced by the presence of Na2SeO3 in a clear dose-related manner. These results indicated that selenite and AFB1 mutually antagonized their ability to cause DNA damage leading to the formation of SCEs. However, selenium didn't completely inhibit induction of SCEs by AFB1 compared to controls. This is first report describing, the protective ability of selenium againist AFB1 genotoxicity on human WBC.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400006Aflatoxin B1seleniumsister-chromatid exchangesgenotoxicitywhole blood cultures |
| spellingShingle | Fatime Geyikoglu Hasan Türkez Protective effect of sodium selenite against the genotoxicity of aflatoxin B1 in human whole blood cultures Brazilian Archives of Biology and Technology Aflatoxin B1 selenium sister-chromatid exchanges genotoxicity whole blood cultures |
| title | Protective effect of sodium selenite against the genotoxicity of aflatoxin B1 in human whole blood cultures |
| title_full | Protective effect of sodium selenite against the genotoxicity of aflatoxin B1 in human whole blood cultures |
| title_fullStr | Protective effect of sodium selenite against the genotoxicity of aflatoxin B1 in human whole blood cultures |
| title_full_unstemmed | Protective effect of sodium selenite against the genotoxicity of aflatoxin B1 in human whole blood cultures |
| title_short | Protective effect of sodium selenite against the genotoxicity of aflatoxin B1 in human whole blood cultures |
| title_sort | protective effect of sodium selenite against the genotoxicity of aflatoxin b1 in human whole blood cultures |
| topic | Aflatoxin B1 selenium sister-chromatid exchanges genotoxicity whole blood cultures |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000400006 |
| work_keys_str_mv | AT fatimegeyikoglu protectiveeffectofsodiumseleniteagainstthegenotoxicityofaflatoxinb1inhumanwholebloodcultures AT hasanturkez protectiveeffectofsodiumseleniteagainstthegenotoxicityofaflatoxinb1inhumanwholebloodcultures |