Differential temporal expression of matrix metalloproteinases following sciatic nerve crush
We previously performed transcriptome sequencing and found that genes for matrix metalloproteinases (MMPs), such as MMP7 and 12, seem to be highly upregulated following peripheral nerve injury, and may be involved in nerve repair. In the present study, we systematically determined the expression lev...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2016-01-01
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| Series: | Neural Regeneration Research |
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| Online Access: | http://www.nrronline.org/article.asp?issn=1673-5374;year=2016;volume=11;issue=7;spage=1165;epage=1171;aulast=Qin |
| _version_ | 1819204651254284288 |
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| author | Jing Qin Guang-bin Zha Jun Yu Hong-hong Zhang Sheng Yi |
| author_facet | Jing Qin Guang-bin Zha Jun Yu Hong-hong Zhang Sheng Yi |
| author_sort | Jing Qin |
| collection | DOAJ |
| description | We previously performed transcriptome sequencing and found that genes for matrix metalloproteinases (MMPs), such as MMP7 and 12, seem to be highly upregulated following peripheral nerve injury, and may be involved in nerve repair. In the present study, we systematically determined the expression levels of MMPs and their regulators at 1, 4, 7 and 14 days after sciatic nerve crush injury. The number of differentially expressed genes was elevated at 4 and 7 days after injury, but decreased at 14 days after injury. Among the differentially expressed genes, those most up-regulated showed fold changes of more than 214, while those most down-regulated exhibited fold changes of more than 2−10. Gene sequencing showed that, at all time points after injury, a variety of MMP genes in the “Inhibition of MMPs” pathway were up-regulated, and their inhibitor genes were down-regulated. Expression of key up- and down-regulated genes was verified by quantitative real-time polymerase chain reaction analysis and found to be consistent with transcriptome sequencing. These results suggest that MMP-related genes are strongly involved in the process of peripheral nerve regeneration. |
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| institution | Directory Open Access Journal |
| issn | 1673-5374 |
| language | English |
| last_indexed | 2024-12-23T04:39:12Z |
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| spelling | doaj.art-be83500782d947a7acb16b740f3ecaa92022-12-21T17:59:48ZengWolters Kluwer Medknow PublicationsNeural Regeneration Research1673-53742016-01-011171165117110.4103/1673-5374.187059Differential temporal expression of matrix metalloproteinases following sciatic nerve crushJing QinGuang-bin ZhaJun YuHong-hong ZhangSheng YiWe previously performed transcriptome sequencing and found that genes for matrix metalloproteinases (MMPs), such as MMP7 and 12, seem to be highly upregulated following peripheral nerve injury, and may be involved in nerve repair. In the present study, we systematically determined the expression levels of MMPs and their regulators at 1, 4, 7 and 14 days after sciatic nerve crush injury. The number of differentially expressed genes was elevated at 4 and 7 days after injury, but decreased at 14 days after injury. Among the differentially expressed genes, those most up-regulated showed fold changes of more than 214, while those most down-regulated exhibited fold changes of more than 2−10. Gene sequencing showed that, at all time points after injury, a variety of MMP genes in the “Inhibition of MMPs” pathway were up-regulated, and their inhibitor genes were down-regulated. Expression of key up- and down-regulated genes was verified by quantitative real-time polymerase chain reaction analysis and found to be consistent with transcriptome sequencing. These results suggest that MMP-related genes are strongly involved in the process of peripheral nerve regeneration.http://www.nrronline.org/article.asp?issn=1673-5374;year=2016;volume=11;issue=7;spage=1165;epage=1171;aulast=Qinnerve regeneration; peripheral nerve injury; sciatic nerve crush; transcriptome sequencing; bioinformatic analysis; canonical pathway; extracellular matrix; matrix metalloproteinase; tissue inhibitors of metalloproteinase; disintegrin; repair; neural regeneration |
| spellingShingle | Jing Qin Guang-bin Zha Jun Yu Hong-hong Zhang Sheng Yi Differential temporal expression of matrix metalloproteinases following sciatic nerve crush Neural Regeneration Research nerve regeneration; peripheral nerve injury; sciatic nerve crush; transcriptome sequencing; bioinformatic analysis; canonical pathway; extracellular matrix; matrix metalloproteinase; tissue inhibitors of metalloproteinase; disintegrin; repair; neural regeneration |
| title | Differential temporal expression of matrix metalloproteinases following sciatic nerve crush |
| title_full | Differential temporal expression of matrix metalloproteinases following sciatic nerve crush |
| title_fullStr | Differential temporal expression of matrix metalloproteinases following sciatic nerve crush |
| title_full_unstemmed | Differential temporal expression of matrix metalloproteinases following sciatic nerve crush |
| title_short | Differential temporal expression of matrix metalloproteinases following sciatic nerve crush |
| title_sort | differential temporal expression of matrix metalloproteinases following sciatic nerve crush |
| topic | nerve regeneration; peripheral nerve injury; sciatic nerve crush; transcriptome sequencing; bioinformatic analysis; canonical pathway; extracellular matrix; matrix metalloproteinase; tissue inhibitors of metalloproteinase; disintegrin; repair; neural regeneration |
| url | http://www.nrronline.org/article.asp?issn=1673-5374;year=2016;volume=11;issue=7;spage=1165;epage=1171;aulast=Qin |
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