Antimicrobial Peptides Relieve Transportation Stress in Ragdoll Cats by Regulating the Gut Microbiota
Transportation is common in cats and often causes stress and intestinal disorders. Antimicrobial peptides (AMPs) exhibit a broad spectrum of antibacterial activity, and they may have the capacity for antioxidant and immune regulation. The objective of this study was to investigate the effects of die...
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MDPI AG
2023-02-01
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| Series: | Metabolites |
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| Online Access: | https://www.mdpi.com/2218-1989/13/3/326 |
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| author | Shansong He Kang Yang Jiawei Wen Tao Kuang Zhihao Cao Lingna Zhang Sufang Han Shiyan Jian Xin Chen Limeng Zhang Jinping Deng Baichuan Deng |
| author_facet | Shansong He Kang Yang Jiawei Wen Tao Kuang Zhihao Cao Lingna Zhang Sufang Han Shiyan Jian Xin Chen Limeng Zhang Jinping Deng Baichuan Deng |
| author_sort | Shansong He |
| collection | DOAJ |
| description | Transportation is common in cats and often causes stress and intestinal disorders. Antimicrobial peptides (AMPs) exhibit a broad spectrum of antibacterial activity, and they may have the capacity for antioxidant and immune regulation. The objective of this study was to investigate the effects of dietary supplementation with AMPs on stress response, gut microbiota and metabolites of cats that have undergone transport stress. A total of 14 Ragdoll cats were randomly allocated into 2 treatments: basal diet (CON) and a basal diet supplemented with 0.3% AMPs. After a 6-week feeding period, all cats were transported for 3 h and, then, fed for another week. The results show that the diarrhea rate of cats was markedly reduced by supplementation with AMPs throughout the trial period (<i>p</i> < 0.05). In addition, AMPs significantly reduced serum cortisol and serum amyloid A (<i>p</i> < 0.05) and increased apolipoprotein 1 after transportation (<i>p</i> < 0.05). Moreover, AMPs reduced the level of inflammatory factors in the serum caused by transportation stress, including tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) (<i>p</i> < 0.05). The AMPs enhanced the activities of glutathione peroxidase (<i>p</i> < 0.01) and superoxide dismutase (<i>p</i> < 0.05). Furthermore, cats fed AMPs had higher levels of branched chain fatty acids (BCFAs) and a relative abundance of <i>Blautia</i> and a lower relative abundance of <i>Negativibacillus</i> after transportation (<i>p</i> < 0.05). The serum metabolome analysis further revealed that AMPs markedly regulated lipid metabolism by upregulating cholic acid expression. In conclusion, AMP supplementation alleviated oxidative stress and inflammatory response in transportation by regulating the gut microbiota and metabolites, thereby relieving stress-induced diarrhea and supporting gut and host health in cats. |
| first_indexed | 2024-03-11T06:11:36Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-11T06:11:36Z |
| publishDate | 2023-02-01 |
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| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-be8505dab1f94a618fced81898f8c6452023-11-17T12:35:56ZengMDPI AGMetabolites2218-19892023-02-0113332610.3390/metabo13030326Antimicrobial Peptides Relieve Transportation Stress in Ragdoll Cats by Regulating the Gut MicrobiotaShansong He0Kang Yang1Jiawei Wen2Tao Kuang3Zhihao Cao4Lingna Zhang5Sufang Han6Shiyan Jian7Xin Chen8Limeng Zhang9Jinping Deng10Baichuan Deng11Guangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaSchool of Medicine, Foshan University, Foshan 528000, ChinaResearch Center of Pet Nutrition, Guangzhou Qingke Biotechnology Co., Ltd., Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Animal Nutrition Control, National Engineering Research Center for Breeding Swine Industry, College of Animal Science, South China Agricultural University, Guangzhou 510642, ChinaTransportation is common in cats and often causes stress and intestinal disorders. Antimicrobial peptides (AMPs) exhibit a broad spectrum of antibacterial activity, and they may have the capacity for antioxidant and immune regulation. The objective of this study was to investigate the effects of dietary supplementation with AMPs on stress response, gut microbiota and metabolites of cats that have undergone transport stress. A total of 14 Ragdoll cats were randomly allocated into 2 treatments: basal diet (CON) and a basal diet supplemented with 0.3% AMPs. After a 6-week feeding period, all cats were transported for 3 h and, then, fed for another week. The results show that the diarrhea rate of cats was markedly reduced by supplementation with AMPs throughout the trial period (<i>p</i> < 0.05). In addition, AMPs significantly reduced serum cortisol and serum amyloid A (<i>p</i> < 0.05) and increased apolipoprotein 1 after transportation (<i>p</i> < 0.05). Moreover, AMPs reduced the level of inflammatory factors in the serum caused by transportation stress, including tumor necrosis factor-alpha (TNF-α) and interleukin-1 beta (IL-1β) (<i>p</i> < 0.05). The AMPs enhanced the activities of glutathione peroxidase (<i>p</i> < 0.01) and superoxide dismutase (<i>p</i> < 0.05). Furthermore, cats fed AMPs had higher levels of branched chain fatty acids (BCFAs) and a relative abundance of <i>Blautia</i> and a lower relative abundance of <i>Negativibacillus</i> after transportation (<i>p</i> < 0.05). The serum metabolome analysis further revealed that AMPs markedly regulated lipid metabolism by upregulating cholic acid expression. In conclusion, AMP supplementation alleviated oxidative stress and inflammatory response in transportation by regulating the gut microbiota and metabolites, thereby relieving stress-induced diarrhea and supporting gut and host health in cats.https://www.mdpi.com/2218-1989/13/3/326transportation stressantimicrobial peptidescatspet foodmicrobiotametabolome |
| spellingShingle | Shansong He Kang Yang Jiawei Wen Tao Kuang Zhihao Cao Lingna Zhang Sufang Han Shiyan Jian Xin Chen Limeng Zhang Jinping Deng Baichuan Deng Antimicrobial Peptides Relieve Transportation Stress in Ragdoll Cats by Regulating the Gut Microbiota Metabolites transportation stress antimicrobial peptides cats pet food microbiota metabolome |
| title | Antimicrobial Peptides Relieve Transportation Stress in Ragdoll Cats by Regulating the Gut Microbiota |
| title_full | Antimicrobial Peptides Relieve Transportation Stress in Ragdoll Cats by Regulating the Gut Microbiota |
| title_fullStr | Antimicrobial Peptides Relieve Transportation Stress in Ragdoll Cats by Regulating the Gut Microbiota |
| title_full_unstemmed | Antimicrobial Peptides Relieve Transportation Stress in Ragdoll Cats by Regulating the Gut Microbiota |
| title_short | Antimicrobial Peptides Relieve Transportation Stress in Ragdoll Cats by Regulating the Gut Microbiota |
| title_sort | antimicrobial peptides relieve transportation stress in ragdoll cats by regulating the gut microbiota |
| topic | transportation stress antimicrobial peptides cats pet food microbiota metabolome |
| url | https://www.mdpi.com/2218-1989/13/3/326 |
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