GENO-TYPING OF M. TUBERCULOSIS ISOLATES WITH MULTIPLE DRUG RESISTANCE CIRCULATING IN THE SOUTHERN REGIONS OF KAZAKHSTAN
Kazakhstan has one of the highest incidence of multiple drug resistant tuberculosis in the world. The goal is to describe isolated of M. tuberculosis circulating in the Southern regions of Kazakhstan basing on MIRU-VNTR-profile and spectrum of gene mutations responsible for resistance to the first a...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | Russian |
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New Terra Publishing House
2015-09-01
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| Series: | Туберкулез и болезни лёгких |
| Subjects: | |
| Online Access: | https://www.tibl-journal.com/jour/article/view/795 |
| _version_ | 1797696707420487680 |
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| author | P. V. Tarlykov D. R. Raiymbek A. Kh. Alenova T. Sh. Abildaev E. M. Ramankulov |
| author_facet | P. V. Tarlykov D. R. Raiymbek A. Kh. Alenova T. Sh. Abildaev E. M. Ramankulov |
| author_sort | P. V. Tarlykov |
| collection | DOAJ |
| description | Kazakhstan has one of the highest incidence of multiple drug resistant tuberculosis in the world. The goal is to describe isolated of M. tuberculosis circulating in the Southern regions of Kazakhstan basing on MIRU-VNTR-profile and spectrum of gene mutations responsible for resistance to the first and second line drugs. 58 clinical isolates of M. tuberculosis with extensive drug resistance were collected. Genetic analysis was conducted by sequencing of genes of rpoB, katG, embB and inhA-fabG of operon and genes of gyrA, gyrB and rrs. The replacement of asparaginic acid with cysteine in the 94th codon of gyr4 gene was found out, which has not been described in literature before. Mutations dominated in the 315th codon of katG (n = 53; 91.4%), in the 531th codon of rpoB (n = 45; 77.6%), in position 1 401 of A/G gene rrs (n = 33; 56.9%) and in the 94th codon of gyrA. MIRU-VNTR typing showed that the majority of isolates belonged to W-Beijing family (n = 53, 94.4%). W-Beijing family often manifests resistance which can explain the increase of drug resistant tuberculosis incidence in the Republic. |
| first_indexed | 2024-03-12T03:30:12Z |
| format | Article |
| id | doaj.art-be8860e9d5b146e6ab1414b02a65fa7b |
| institution | Directory Open Access Journal |
| issn | 2075-1230 2542-1506 |
| language | Russian |
| last_indexed | 2024-03-12T03:30:12Z |
| publishDate | 2015-09-01 |
| publisher | New Terra Publishing House |
| record_format | Article |
| series | Туберкулез и болезни лёгких |
| spelling | doaj.art-be8860e9d5b146e6ab1414b02a65fa7b2023-09-03T13:29:15ZrusNew Terra Publishing HouseТуберкулез и болезни лёгких2075-12302542-15062015-09-01094146795GENO-TYPING OF M. TUBERCULOSIS ISOLATES WITH MULTIPLE DRUG RESISTANCE CIRCULATING IN THE SOUTHERN REGIONS OF KAZAKHSTANP. V. Tarlykov0D. R. Raiymbek1A. Kh. Alenova2T. Sh. Abildaev3E. M. Ramankulov4Национальный центр биотехнологии КН МОН РКНациональный центр биотехнологии КН МОН РК, г. Астана, КазахстанНациональный центр проблем туберкулеза МЗ РК, г. Астана, КазахстанНациональный центр проблем туберкулеза МЗ РК, г. Астана, КазахстанНациональный центр биотехнологии КН МОН РК, г. Астана, КазахстанKazakhstan has one of the highest incidence of multiple drug resistant tuberculosis in the world. The goal is to describe isolated of M. tuberculosis circulating in the Southern regions of Kazakhstan basing on MIRU-VNTR-profile and spectrum of gene mutations responsible for resistance to the first and second line drugs. 58 clinical isolates of M. tuberculosis with extensive drug resistance were collected. Genetic analysis was conducted by sequencing of genes of rpoB, katG, embB and inhA-fabG of operon and genes of gyrA, gyrB and rrs. The replacement of asparaginic acid with cysteine in the 94th codon of gyr4 gene was found out, which has not been described in literature before. Mutations dominated in the 315th codon of katG (n = 53; 91.4%), in the 531th codon of rpoB (n = 45; 77.6%), in position 1 401 of A/G gene rrs (n = 33; 56.9%) and in the 94th codon of gyrA. MIRU-VNTR typing showed that the majority of isolates belonged to W-Beijing family (n = 53, 94.4%). W-Beijing family often manifests resistance which can explain the increase of drug resistant tuberculosis incidence in the Republic.https://www.tibl-journal.com/jour/article/view/795туберкулездиагностикалекарственная чувствительностьлекарственная устойчивость возбудителя |
| spellingShingle | P. V. Tarlykov D. R. Raiymbek A. Kh. Alenova T. Sh. Abildaev E. M. Ramankulov GENO-TYPING OF M. TUBERCULOSIS ISOLATES WITH MULTIPLE DRUG RESISTANCE CIRCULATING IN THE SOUTHERN REGIONS OF KAZAKHSTAN Туберкулез и болезни лёгких туберкулез диагностика лекарственная чувствительность лекарственная устойчивость возбудителя |
| title | GENO-TYPING OF M. TUBERCULOSIS ISOLATES WITH MULTIPLE DRUG RESISTANCE CIRCULATING IN THE SOUTHERN REGIONS OF KAZAKHSTAN |
| title_full | GENO-TYPING OF M. TUBERCULOSIS ISOLATES WITH MULTIPLE DRUG RESISTANCE CIRCULATING IN THE SOUTHERN REGIONS OF KAZAKHSTAN |
| title_fullStr | GENO-TYPING OF M. TUBERCULOSIS ISOLATES WITH MULTIPLE DRUG RESISTANCE CIRCULATING IN THE SOUTHERN REGIONS OF KAZAKHSTAN |
| title_full_unstemmed | GENO-TYPING OF M. TUBERCULOSIS ISOLATES WITH MULTIPLE DRUG RESISTANCE CIRCULATING IN THE SOUTHERN REGIONS OF KAZAKHSTAN |
| title_short | GENO-TYPING OF M. TUBERCULOSIS ISOLATES WITH MULTIPLE DRUG RESISTANCE CIRCULATING IN THE SOUTHERN REGIONS OF KAZAKHSTAN |
| title_sort | geno typing of m tuberculosis isolates with multiple drug resistance circulating in the southern regions of kazakhstan |
| topic | туберкулез диагностика лекарственная чувствительность лекарственная устойчивость возбудителя |
| url | https://www.tibl-journal.com/jour/article/view/795 |
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