Arginine Deiminase and Biotin Metabolism Signaling Pathways Play an Important Role in Human-Derived Serotype V, ST1 Streptococcus agalactiae Virulent Strain upon Infected Tilapia
Our previous study showed that human-derived Streptococcus agalactiae (serotype V) could infect tilapia, but the mechanism underlying the cross-species infection remains unrecognized. In this study, a multi-omics analysis was performed on human-derived S.agalactiae strain NNA048 (virulent to tilapia...
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MDPI AG
2020-05-01
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| Online Access: | https://www.mdpi.com/2076-2615/10/5/849 |
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| author | Yu Liu Liping Li Zhiping Luo Rui Wang Ting Huang Wanwen Liang Qunhong Gu Fangzhao Yu Ming Chen |
| author_facet | Yu Liu Liping Li Zhiping Luo Rui Wang Ting Huang Wanwen Liang Qunhong Gu Fangzhao Yu Ming Chen |
| author_sort | Yu Liu |
| collection | DOAJ |
| description | Our previous study showed that human-derived Streptococcus agalactiae (serotype V) could infect tilapia, but the mechanism underlying the cross-species infection remains unrecognized. In this study, a multi-omics analysis was performed on human-derived S.agalactiae strain NNA048 (virulent to tilapia, serotype V, ST1) and human-derived S.agalactiae strain NNA038 (non-virulent to tilapia, serotype V, ST1). The results showed that 907 genes (504 up/403 down) and 89 proteins (51 up/38 down) were differentially expressed (p < 0.05) between NNA038 and NNA048. Among them, 56 genes (proteins) were altered with similar trends at both mRNA and protein levels. Functional annotation of them showed that the main differences were enriched in the arginine deiminase system signaling pathway and biotin metabolism signaling pathway: gdhA, glnA, ASL, ADI, OTC, arcC, FabF, FabG, FabZ, BioB and BirA genes may have been important factors leading to the pathogenicity differences between NNA038 and NNA048. We aimed to provide a comprehensive analysis of the human-derived serotype V ST1 S.agalactiae strains, which were virulent and non-virulent to tilapia, and provide a more comprehensive understanding of the virulence mechanism. |
| first_indexed | 2024-03-10T19:49:57Z |
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| id | doaj.art-be8b1313abe948a4a8be04862b6bd7b3 |
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| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T19:49:57Z |
| publishDate | 2020-05-01 |
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| series | Animals |
| spelling | doaj.art-be8b1313abe948a4a8be04862b6bd7b32023-11-20T00:28:31ZengMDPI AGAnimals2076-26152020-05-0110584910.3390/ani10050849Arginine Deiminase and Biotin Metabolism Signaling Pathways Play an Important Role in Human-Derived Serotype V, ST1 Streptococcus agalactiae Virulent Strain upon Infected TilapiaYu Liu0Liping Li1Zhiping Luo2Rui Wang3Ting Huang4Wanwen Liang5Qunhong Gu6Fangzhao Yu7Ming Chen8Guangxi Academy of Fishery Sciences, Nanning 530021, ChinaGuangxi Academy of Fishery Sciences, Nanning 530021, ChinaZhuhai modern agriculture development center, Zhuhai 519000, ChinaGuangxi Academy of Fishery Sciences, Nanning 530021, ChinaGuangxi Academy of Fishery Sciences, Nanning 530021, ChinaGuangxi Academy of Fishery Sciences, Nanning 530021, ChinaZhuhai modern agriculture development center, Zhuhai 519000, ChinaZhuhai modern agriculture development center, Zhuhai 519000, ChinaGuangxi Academy of Fishery Sciences, Nanning 530021, ChinaOur previous study showed that human-derived Streptococcus agalactiae (serotype V) could infect tilapia, but the mechanism underlying the cross-species infection remains unrecognized. In this study, a multi-omics analysis was performed on human-derived S.agalactiae strain NNA048 (virulent to tilapia, serotype V, ST1) and human-derived S.agalactiae strain NNA038 (non-virulent to tilapia, serotype V, ST1). The results showed that 907 genes (504 up/403 down) and 89 proteins (51 up/38 down) were differentially expressed (p < 0.05) between NNA038 and NNA048. Among them, 56 genes (proteins) were altered with similar trends at both mRNA and protein levels. Functional annotation of them showed that the main differences were enriched in the arginine deiminase system signaling pathway and biotin metabolism signaling pathway: gdhA, glnA, ASL, ADI, OTC, arcC, FabF, FabG, FabZ, BioB and BirA genes may have been important factors leading to the pathogenicity differences between NNA038 and NNA048. We aimed to provide a comprehensive analysis of the human-derived serotype V ST1 S.agalactiae strains, which were virulent and non-virulent to tilapia, and provide a more comprehensive understanding of the virulence mechanism.https://www.mdpi.com/2076-2615/10/5/849Streptococcus agalactiaeOreochromis niloticuscross-species infectiontranscriptomeproteome |
| spellingShingle | Yu Liu Liping Li Zhiping Luo Rui Wang Ting Huang Wanwen Liang Qunhong Gu Fangzhao Yu Ming Chen Arginine Deiminase and Biotin Metabolism Signaling Pathways Play an Important Role in Human-Derived Serotype V, ST1 Streptococcus agalactiae Virulent Strain upon Infected Tilapia Animals Streptococcus agalactiae Oreochromis niloticus cross-species infection transcriptome proteome |
| title | Arginine Deiminase and Biotin Metabolism Signaling Pathways Play an Important Role in Human-Derived Serotype V, ST1 Streptococcus agalactiae Virulent Strain upon Infected Tilapia |
| title_full | Arginine Deiminase and Biotin Metabolism Signaling Pathways Play an Important Role in Human-Derived Serotype V, ST1 Streptococcus agalactiae Virulent Strain upon Infected Tilapia |
| title_fullStr | Arginine Deiminase and Biotin Metabolism Signaling Pathways Play an Important Role in Human-Derived Serotype V, ST1 Streptococcus agalactiae Virulent Strain upon Infected Tilapia |
| title_full_unstemmed | Arginine Deiminase and Biotin Metabolism Signaling Pathways Play an Important Role in Human-Derived Serotype V, ST1 Streptococcus agalactiae Virulent Strain upon Infected Tilapia |
| title_short | Arginine Deiminase and Biotin Metabolism Signaling Pathways Play an Important Role in Human-Derived Serotype V, ST1 Streptococcus agalactiae Virulent Strain upon Infected Tilapia |
| title_sort | arginine deiminase and biotin metabolism signaling pathways play an important role in human derived serotype v st1 streptococcus agalactiae virulent strain upon infected tilapia |
| topic | Streptococcus agalactiae Oreochromis niloticus cross-species infection transcriptome proteome |
| url | https://www.mdpi.com/2076-2615/10/5/849 |
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