Genome-Wide Identification and Expression Analysis of <i>AS2</i> Genes in <i>Brassica rapa</i> Reveal Their Potential Roles in Abiotic Stress
The <i>ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES</i> (<i>AS2/LOB</i>) gene family plays a pivotal role in plant growth, induction of phytohormones, and the abiotic stress response. However, the <i>AS2</i> gene family in <i>Brassica rapa</i> has yet t...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/13/10534 |
| Summary: | The <i>ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES</i> (<i>AS2/LOB</i>) gene family plays a pivotal role in plant growth, induction of phytohormones, and the abiotic stress response. However, the <i>AS2</i> gene family in <i>Brassica rapa</i> has yet to be investigated. In this study, we identified 62 <i>AS2</i> genes in the <i>B. rapa</i> genome, which were classified into six subfamilies and distributed across 10 chromosomes. Sequence analysis of <i>BrAS2</i> promotors showed that there are several typical <i>cis</i>-elements involved in abiotic stress tolerance and stress-related hormone response. Tissue-specific expression analysis showed that <i>BrAS2-47</i> exhibited ubiquitous expression in all tissues, indicating it may be involved in many biological processes. Gene expression analysis showed that the expressions of <i>BrAS2-47</i> and <i>BrAS2-10</i> were significantly downregulated under cold stress, heat stress, drought stress, and salt stress, while <i>BrAS2-58</i> expression was significantly upregulated under heat stress. RT-qPCR also confirmed that the expression of <i>BrAS2-47</i> and <i>BrAS2-10</i> was significantly downregulated under cold stress, drought stress, and salt stress, and in addition <i>BrAS2-56</i> and <i>BrAS2-4</i> also changed significantly under the three stresses. In addition, protein–protein interaction (PPI) network analysis revealed that the <i>Arabidopsis thaliana</i> genes <i>AT5G67420</i> (homologous gene of <i>BrAS2-47</i> and <i>BrAS2-10</i>) and <i>AT3G49940</i> (homologous gene of <i>BrAS2-58</i>) can interact with NIN-like protein 7 (NLP7), which has been previously reported to play a role in resistance to adverse environments. In summary, our findings suggest that among the <i>BrAS2</i> gene family, <i>BrAS2-47</i> and <i>BrAS2-10</i> have the most potential for the regulation of abiotic stress tolerance. These results will facilitate future functional investigations of <i>BrAS2</i> genes in <i>B. rapa</i>. |
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| ISSN: | 1661-6596 1422-0067 |