Genome-Wide Identification and Expression Analysis of <i>AS2</i> Genes in <i>Brassica rapa</i> Reveal Their Potential Roles in Abiotic Stress
The <i>ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES</i> (<i>AS2/LOB</i>) gene family plays a pivotal role in plant growth, induction of phytohormones, and the abiotic stress response. However, the <i>AS2</i> gene family in <i>Brassica rapa</i> has yet t...
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| author | Qiwei Jiang Xiaoyu Wu Xiaoyu Zhang Zhaojing Ji Yunyun Cao Qiaohong Duan Jiabao Huang |
| author_facet | Qiwei Jiang Xiaoyu Wu Xiaoyu Zhang Zhaojing Ji Yunyun Cao Qiaohong Duan Jiabao Huang |
| author_sort | Qiwei Jiang |
| collection | DOAJ |
| description | The <i>ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES</i> (<i>AS2/LOB</i>) gene family plays a pivotal role in plant growth, induction of phytohormones, and the abiotic stress response. However, the <i>AS2</i> gene family in <i>Brassica rapa</i> has yet to be investigated. In this study, we identified 62 <i>AS2</i> genes in the <i>B. rapa</i> genome, which were classified into six subfamilies and distributed across 10 chromosomes. Sequence analysis of <i>BrAS2</i> promotors showed that there are several typical <i>cis</i>-elements involved in abiotic stress tolerance and stress-related hormone response. Tissue-specific expression analysis showed that <i>BrAS2-47</i> exhibited ubiquitous expression in all tissues, indicating it may be involved in many biological processes. Gene expression analysis showed that the expressions of <i>BrAS2-47</i> and <i>BrAS2-10</i> were significantly downregulated under cold stress, heat stress, drought stress, and salt stress, while <i>BrAS2-58</i> expression was significantly upregulated under heat stress. RT-qPCR also confirmed that the expression of <i>BrAS2-47</i> and <i>BrAS2-10</i> was significantly downregulated under cold stress, drought stress, and salt stress, and in addition <i>BrAS2-56</i> and <i>BrAS2-4</i> also changed significantly under the three stresses. In addition, protein–protein interaction (PPI) network analysis revealed that the <i>Arabidopsis thaliana</i> genes <i>AT5G67420</i> (homologous gene of <i>BrAS2-47</i> and <i>BrAS2-10</i>) and <i>AT3G49940</i> (homologous gene of <i>BrAS2-58</i>) can interact with NIN-like protein 7 (NLP7), which has been previously reported to play a role in resistance to adverse environments. In summary, our findings suggest that among the <i>BrAS2</i> gene family, <i>BrAS2-47</i> and <i>BrAS2-10</i> have the most potential for the regulation of abiotic stress tolerance. These results will facilitate future functional investigations of <i>BrAS2</i> genes in <i>B. rapa</i>. |
| first_indexed | 2024-03-11T01:40:21Z |
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| spelling | doaj.art-be8d85cea2344304b1f904f24df9028d2023-11-18T16:39:37ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-0124131053410.3390/ijms241310534Genome-Wide Identification and Expression Analysis of <i>AS2</i> Genes in <i>Brassica rapa</i> Reveal Their Potential Roles in Abiotic StressQiwei Jiang0Xiaoyu Wu1Xiaoyu Zhang2Zhaojing Ji3Yunyun Cao4Qiaohong Duan5Jiabao Huang6College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaCollege of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an 271000, ChinaThe <i>ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES</i> (<i>AS2/LOB</i>) gene family plays a pivotal role in plant growth, induction of phytohormones, and the abiotic stress response. However, the <i>AS2</i> gene family in <i>Brassica rapa</i> has yet to be investigated. In this study, we identified 62 <i>AS2</i> genes in the <i>B. rapa</i> genome, which were classified into six subfamilies and distributed across 10 chromosomes. Sequence analysis of <i>BrAS2</i> promotors showed that there are several typical <i>cis</i>-elements involved in abiotic stress tolerance and stress-related hormone response. Tissue-specific expression analysis showed that <i>BrAS2-47</i> exhibited ubiquitous expression in all tissues, indicating it may be involved in many biological processes. Gene expression analysis showed that the expressions of <i>BrAS2-47</i> and <i>BrAS2-10</i> were significantly downregulated under cold stress, heat stress, drought stress, and salt stress, while <i>BrAS2-58</i> expression was significantly upregulated under heat stress. RT-qPCR also confirmed that the expression of <i>BrAS2-47</i> and <i>BrAS2-10</i> was significantly downregulated under cold stress, drought stress, and salt stress, and in addition <i>BrAS2-56</i> and <i>BrAS2-4</i> also changed significantly under the three stresses. In addition, protein–protein interaction (PPI) network analysis revealed that the <i>Arabidopsis thaliana</i> genes <i>AT5G67420</i> (homologous gene of <i>BrAS2-47</i> and <i>BrAS2-10</i>) and <i>AT3G49940</i> (homologous gene of <i>BrAS2-58</i>) can interact with NIN-like protein 7 (NLP7), which has been previously reported to play a role in resistance to adverse environments. In summary, our findings suggest that among the <i>BrAS2</i> gene family, <i>BrAS2-47</i> and <i>BrAS2-10</i> have the most potential for the regulation of abiotic stress tolerance. These results will facilitate future functional investigations of <i>BrAS2</i> genes in <i>B. rapa</i>.https://www.mdpi.com/1422-0067/24/13/10534<i>Brassica rapa</i><i>AS2</i>bioinformatics analysisexpression profileabiotic stress |
| spellingShingle | Qiwei Jiang Xiaoyu Wu Xiaoyu Zhang Zhaojing Ji Yunyun Cao Qiaohong Duan Jiabao Huang Genome-Wide Identification and Expression Analysis of <i>AS2</i> Genes in <i>Brassica rapa</i> Reveal Their Potential Roles in Abiotic Stress International Journal of Molecular Sciences <i>Brassica rapa</i> <i>AS2</i> bioinformatics analysis expression profile abiotic stress |
| title | Genome-Wide Identification and Expression Analysis of <i>AS2</i> Genes in <i>Brassica rapa</i> Reveal Their Potential Roles in Abiotic Stress |
| title_full | Genome-Wide Identification and Expression Analysis of <i>AS2</i> Genes in <i>Brassica rapa</i> Reveal Their Potential Roles in Abiotic Stress |
| title_fullStr | Genome-Wide Identification and Expression Analysis of <i>AS2</i> Genes in <i>Brassica rapa</i> Reveal Their Potential Roles in Abiotic Stress |
| title_full_unstemmed | Genome-Wide Identification and Expression Analysis of <i>AS2</i> Genes in <i>Brassica rapa</i> Reveal Their Potential Roles in Abiotic Stress |
| title_short | Genome-Wide Identification and Expression Analysis of <i>AS2</i> Genes in <i>Brassica rapa</i> Reveal Their Potential Roles in Abiotic Stress |
| title_sort | genome wide identification and expression analysis of i as2 i genes in i brassica rapa i reveal their potential roles in abiotic stress |
| topic | <i>Brassica rapa</i> <i>AS2</i> bioinformatics analysis expression profile abiotic stress |
| url | https://www.mdpi.com/1422-0067/24/13/10534 |
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