Anticancerous Effects of Polygonum Avicular Exert through Fas Ligand and Bcl2
Background: Herbal medicine is a new complementary strategy for treating diseases such as cancer. However, its mechanism of action is not clear yet. This study investigates antitumor efficacy of Polygonum avicular extract on MCF-7 cell line. Methods: MCF-7 cells were treated with different concentr...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2013-12-01
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| Series: | Basic & Clinical Cancer Research |
| Subjects: | |
| Online Access: | https://bccr.tums.ac.ir/index.php/bccrj/article/view/38 |
| _version_ | 1818872886196174848 |
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| author | Motahareh Mortazavi Mehryar Habibi Roudkenar Abbas Delazar Raheleh Halabian Sara Ansari Zohreh Alizadeh Amaneh Mohammadi Roushandeh |
| author_facet | Motahareh Mortazavi Mehryar Habibi Roudkenar Abbas Delazar Raheleh Halabian Sara Ansari Zohreh Alizadeh Amaneh Mohammadi Roushandeh |
| author_sort | Motahareh Mortazavi |
| collection | DOAJ |
| description | Background: Herbal medicine is a new complementary strategy for treating diseases such as cancer. However, its mechanism of action is not clear yet. This study investigates antitumor efficacy of Polygonum avicular extract on MCF-7 cell line.
Methods: MCF-7 cells were treated with different concentrations of (50, 100, 150, 200, 250, 300,350 400 ng/µl) Polygonum avicular at different time intervals (6, 12, 24, and 48 hrs). In order to evaluate the expression level of FasL and Bcl2 genes, RT-PCR technique was employed.
Results: The obtained results demonstrate that expression of FasL gene was increased in Polygonum avicular treated cells in comparison to control group. Down-regulation of Bcl2 expression was observed during the treatment.
Conclusion: Polygonum aviculare herbal extract may induce apoptosis through up-regulation of FasL and down-regulation of Bcl2 gene, however, its complete mechanism of action must be elucidated. |
| first_indexed | 2024-12-19T12:45:56Z |
| format | Article |
| id | doaj.art-be91e8f0406f49558974452d878a1d20 |
| institution | Directory Open Access Journal |
| issn | 2228-6527 2228-5466 |
| language | English |
| last_indexed | 2024-12-19T12:45:56Z |
| publishDate | 2013-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Basic & Clinical Cancer Research |
| spelling | doaj.art-be91e8f0406f49558974452d878a1d202022-12-21T20:20:46ZengTehran University of Medical SciencesBasic & Clinical Cancer Research2228-65272228-54662013-12-0141&2Anticancerous Effects of Polygonum Avicular Exert through Fas Ligand and Bcl2Motahareh Mortazavi0Mehryar Habibi Roudkenar1Abbas Delazar2Raheleh Halabian3Sara Ansari4Zohreh Alizadeh5Amaneh Mohammadi Roushandeh6Anatomical Sciences Department, Medicine Faculty, Hamadan University of Medical Sciences, Hamadan, IranBlood Transfusion Research Center, High Institute in Research Blood Transfusion Organization, Tehran, IranPharmacognosy Department, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, IranBlood Transfusion Research Center, High Institute in Research Blood Transfusion Organization, Tehran, IranAnatomical Sciences Department, Medicine Faculty, Hamadan University of Medical Sciences, Hamadan, IranAnatomical Sciences Department, Medicine Faculty, Hamadan University of Medical Sciences, Hamadan, IranAnatomical Sciences Department, Medicine Faculty, Hamadan University of Medical Sciences, Hamadan, IranBackground: Herbal medicine is a new complementary strategy for treating diseases such as cancer. However, its mechanism of action is not clear yet. This study investigates antitumor efficacy of Polygonum avicular extract on MCF-7 cell line. Methods: MCF-7 cells were treated with different concentrations of (50, 100, 150, 200, 250, 300,350 400 ng/µl) Polygonum avicular at different time intervals (6, 12, 24, and 48 hrs). In order to evaluate the expression level of FasL and Bcl2 genes, RT-PCR technique was employed. Results: The obtained results demonstrate that expression of FasL gene was increased in Polygonum avicular treated cells in comparison to control group. Down-regulation of Bcl2 expression was observed during the treatment. Conclusion: Polygonum aviculare herbal extract may induce apoptosis through up-regulation of FasL and down-regulation of Bcl2 gene, however, its complete mechanism of action must be elucidated.https://bccr.tums.ac.ir/index.php/bccrj/article/view/38Breast cancerHerbal medicinePolygonum aviculare |
| spellingShingle | Motahareh Mortazavi Mehryar Habibi Roudkenar Abbas Delazar Raheleh Halabian Sara Ansari Zohreh Alizadeh Amaneh Mohammadi Roushandeh Anticancerous Effects of Polygonum Avicular Exert through Fas Ligand and Bcl2 Basic & Clinical Cancer Research Breast cancer Herbal medicine Polygonum aviculare |
| title | Anticancerous Effects of Polygonum Avicular Exert through Fas Ligand and Bcl2 |
| title_full | Anticancerous Effects of Polygonum Avicular Exert through Fas Ligand and Bcl2 |
| title_fullStr | Anticancerous Effects of Polygonum Avicular Exert through Fas Ligand and Bcl2 |
| title_full_unstemmed | Anticancerous Effects of Polygonum Avicular Exert through Fas Ligand and Bcl2 |
| title_short | Anticancerous Effects of Polygonum Avicular Exert through Fas Ligand and Bcl2 |
| title_sort | anticancerous effects of polygonum avicular exert through fas ligand and bcl2 |
| topic | Breast cancer Herbal medicine Polygonum aviculare |
| url | https://bccr.tums.ac.ir/index.php/bccrj/article/view/38 |
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