Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV-<i>neu</i>T) in Mice with Transplanted Salivary Gland Carcinoma Cells
The survival rate for head and neck cancer patients has not substantially changed in the last two decades. We previously showed that two rV-<i>neu</i>T intratumoral injections induced an efficient antitumor response and rejection of transplanted Neu (rat <i>ErbB2/neu</i> onco...
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MDPI AG
2020-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/5/1417 |
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| author | Chiara Focaccetti Monica Benvenuto Sara Ciuffa Sara Fazi Manuel Scimeca Alessandra Nardi Martino Tony Miele Andrea Battisti Elena Bonanno Andrea Modesti Laura Masuelli Roberto Bei |
| author_facet | Chiara Focaccetti Monica Benvenuto Sara Ciuffa Sara Fazi Manuel Scimeca Alessandra Nardi Martino Tony Miele Andrea Battisti Elena Bonanno Andrea Modesti Laura Masuelli Roberto Bei |
| author_sort | Chiara Focaccetti |
| collection | DOAJ |
| description | The survival rate for head and neck cancer patients has not substantially changed in the last two decades. We previously showed that two rV-<i>neu</i>T intratumoral injections induced an efficient antitumor response and rejection of transplanted Neu (rat <i>ErbB2/neu</i> oncogene-encoded protein)-overexpressing salivary gland tumor cells in BALB-<i>neu</i>T mice (BALB/c mice transgenic for the rat <i>ErbB2/neu</i> oncogene). However, reiterated poxviral vaccinations increase neutralizing antibodies to viral proteins in humans that prevent immune response against the recombinant antigen expressed by the virus. Curcumin (CUR) is a polyphenol with antineoplastic and immunomodulatory properties. The aim of this study was to employ CUR administration to boost the anti-Neu immune response and anticancer activity induced by one rV-<i>neu</i>T intratumoral vaccination in BALB-<i>neu</i>T mice. Here, we demonstrated that the combined rV-n<i>eu</i>T+CUR treatment was more effective at reducing tumor growth and increasing mouse survival, anti-Neu humoral response, and IFN-γ/IL-2 T-cell release in vitro than the individual treatment. rV-<i>neu</i>T+CUR-treated mice showed an increased infiltration of CD4<sup>+</sup>/CD8<sup>+</sup> T lymphocytes within the tumor as compared to those that received the individual treatment. Overall, CUR enhanced the antitumoral effect and immune response to Neu induced by the rV-<i>neu</i>T vaccine in mice. Thus, the combined treatment might represent a successful strategy to target ErbB2/Neu-overexpressing tumors. |
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| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T19:49:57Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-be953dcd15284a2997bf38d6b288a7612023-11-20T00:29:21ZengMDPI AGNutrients2072-66432020-05-01125141710.3390/nu12051417Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV-<i>neu</i>T) in Mice with Transplanted Salivary Gland Carcinoma CellsChiara Focaccetti0Monica Benvenuto1Sara Ciuffa2Sara Fazi3Manuel Scimeca4Alessandra Nardi5Martino Tony Miele6Andrea Battisti7Elena Bonanno8Andrea Modesti9Laura Masuelli10Roberto Bei11Department of Human Science and Promotion of the Quality of Life, San Raffaele University Rome, Via di Val Cannuta 247, 00166 Rome, ItalyDepartment of Clinical Sciences and Translational Medicine, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, ItalyDepartment of Clinical Sciences and Translational Medicine, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, ItalyDepartment of Experimental Medicine, “Sapienza” University of Rome, Viale Regina Elena 324, 00161 Rome, ItalyDepartment of Human Science and Promotion of the Quality of Life, San Raffaele University Rome, Via di Val Cannuta 247, 00166 Rome, ItalyDepartment of Mathematics, University of Rome “Tor Vergata”, Via della Ricerca Scientifica 1, 00133 Rome, ItalyDepartment of Experimental Medicine, University of Rome Tor Vergata, Via Montpellier 1, 00133 Rome, ItalyMaxillo Facial Oncologic and Reconstructive Unit, “Sapienza” University of Rome, Policlinico Umberto I, 00161 Rome, ItalyDepartment of Biomedicine and Prevention, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, ItalyDepartment of Clinical Sciences and Translational Medicine, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, ItalyDepartment of Experimental Medicine, “Sapienza” University of Rome, Viale Regina Elena 324, 00161 Rome, ItalyDepartment of Clinical Sciences and Translational Medicine, University of Rome “Tor Vergata”, Via Montpellier 1, 00133 Rome, ItalyThe survival rate for head and neck cancer patients has not substantially changed in the last two decades. We previously showed that two rV-<i>neu</i>T intratumoral injections induced an efficient antitumor response and rejection of transplanted Neu (rat <i>ErbB2/neu</i> oncogene-encoded protein)-overexpressing salivary gland tumor cells in BALB-<i>neu</i>T mice (BALB/c mice transgenic for the rat <i>ErbB2/neu</i> oncogene). However, reiterated poxviral vaccinations increase neutralizing antibodies to viral proteins in humans that prevent immune response against the recombinant antigen expressed by the virus. Curcumin (CUR) is a polyphenol with antineoplastic and immunomodulatory properties. The aim of this study was to employ CUR administration to boost the anti-Neu immune response and anticancer activity induced by one rV-<i>neu</i>T intratumoral vaccination in BALB-<i>neu</i>T mice. Here, we demonstrated that the combined rV-n<i>eu</i>T+CUR treatment was more effective at reducing tumor growth and increasing mouse survival, anti-Neu humoral response, and IFN-γ/IL-2 T-cell release in vitro than the individual treatment. rV-<i>neu</i>T+CUR-treated mice showed an increased infiltration of CD4<sup>+</sup>/CD8<sup>+</sup> T lymphocytes within the tumor as compared to those that received the individual treatment. Overall, CUR enhanced the antitumoral effect and immune response to Neu induced by the rV-<i>neu</i>T vaccine in mice. Thus, the combined treatment might represent a successful strategy to target ErbB2/Neu-overexpressing tumors.https://www.mdpi.com/2072-6643/12/5/1417vaccinecancerErbB2/Neucurcuminhead and neckimmune response |
| spellingShingle | Chiara Focaccetti Monica Benvenuto Sara Ciuffa Sara Fazi Manuel Scimeca Alessandra Nardi Martino Tony Miele Andrea Battisti Elena Bonanno Andrea Modesti Laura Masuelli Roberto Bei Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV-<i>neu</i>T) in Mice with Transplanted Salivary Gland Carcinoma Cells Nutrients vaccine cancer ErbB2/Neu curcumin head and neck immune response |
| title | Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV-<i>neu</i>T) in Mice with Transplanted Salivary Gland Carcinoma Cells |
| title_full | Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV-<i>neu</i>T) in Mice with Transplanted Salivary Gland Carcinoma Cells |
| title_fullStr | Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV-<i>neu</i>T) in Mice with Transplanted Salivary Gland Carcinoma Cells |
| title_full_unstemmed | Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV-<i>neu</i>T) in Mice with Transplanted Salivary Gland Carcinoma Cells |
| title_short | Curcumin Enhances the Antitumoral Effect Induced by the Recombinant Vaccinia Neu Vaccine (rV-<i>neu</i>T) in Mice with Transplanted Salivary Gland Carcinoma Cells |
| title_sort | curcumin enhances the antitumoral effect induced by the recombinant vaccinia neu vaccine rv i neu i t in mice with transplanted salivary gland carcinoma cells |
| topic | vaccine cancer ErbB2/Neu curcumin head and neck immune response |
| url | https://www.mdpi.com/2072-6643/12/5/1417 |
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