Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis
Osteoarthritis (OA) is a chronic disease that affects articular cartilage, causing its degeneration. Although OA is one of the most prevalent pathologies globally, there are no definitive treatments available. Recently, research has focused on elucidating the complex interplay that takes place betwe...
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MDPI AG
2022-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/6/3355 |
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| author | Carmen Núñez-Carro Margarita Blanco-Blanco Tatiana Montoya Karla M. Villagrán-Andrade Tamara Hermida-Gómez Francisco J. Blanco María C. de Andrés |
| author_facet | Carmen Núñez-Carro Margarita Blanco-Blanco Tatiana Montoya Karla M. Villagrán-Andrade Tamara Hermida-Gómez Francisco J. Blanco María C. de Andrés |
| author_sort | Carmen Núñez-Carro |
| collection | DOAJ |
| description | Osteoarthritis (OA) is a chronic disease that affects articular cartilage, causing its degeneration. Although OA is one of the most prevalent pathologies globally, there are no definitive treatments available. Recently, research has focused on elucidating the complex interplay that takes place between inflammatory processes and epigenetic regulation, showing that histone post-translational modifications (PTMs) can exert a pronounced effect on the expression of OA-related genes. OA chondrocytes enhance the production of interleukin 1β (IL-1β) and interleukin 8 (IL-8), which are epigenetically regulated. These cytokines upregulate the synthesis of matrix metalloproteinases (MMPs) and aggrecanases, which promote the extracellular matrix (ECM) destruction. This motivates the study of histone PTMs to investigate the epigenetic regulation of proinflammatory molecules, but the absence of specific protocols to extract histones from human articular cartilage has complicated this task. The lack of effective methods can be explained by the structural complexity and low cellularity of this tissue, which are responsible for the biomechanical properties that allow the movement of the joint but also complicate histone isolation. Here, we provide a histone extraction procedure specifically adapted for cryopreserved human articular cartilage that can be useful to understand epigenetic regulation in OA and accelerate the search for novel strategies. |
| first_indexed | 2024-03-09T19:41:43Z |
| format | Article |
| id | doaj.art-be984263fcae45da8c00dae471a5bc5f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T19:41:43Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-be984263fcae45da8c00dae471a5bc5f2023-11-24T01:38:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-03-01236335510.3390/ijms23063355Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in OsteoarthritisCarmen Núñez-Carro0Margarita Blanco-Blanco1Tatiana Montoya2Karla M. Villagrán-Andrade3Tamara Hermida-Gómez4Francisco J. Blanco5María C. de Andrés6Unidad de Epigenética, Grupo de Investigación en Reumatología (GIR), Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario, de A Coruña (CHUAC), Sergas, 15006 A Coruña, SpainUnidad de Epigenética, Grupo de Investigación en Reumatología (GIR), Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario, de A Coruña (CHUAC), Sergas, 15006 A Coruña, SpainDepartment of Pharmacology, Faculty of Pharmacy, Universidad de Sevilla, 41012 Sevilla, SpainUnidad de Epigenética, Grupo de Investigación en Reumatología (GIR), Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario, de A Coruña (CHUAC), Sergas, 15006 A Coruña, SpainUnidad de Epigenética, Grupo de Investigación en Reumatología (GIR), Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario, de A Coruña (CHUAC), Sergas, 15006 A Coruña, SpainUnidad de Epigenética, Grupo de Investigación en Reumatología (GIR), Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario, de A Coruña (CHUAC), Sergas, 15006 A Coruña, SpainUnidad de Epigenética, Grupo de Investigación en Reumatología (GIR), Instituto de Investigación Biomédica de A Coruña (INIBIC), Complexo Hospitalario Universitario, de A Coruña (CHUAC), Sergas, 15006 A Coruña, SpainOsteoarthritis (OA) is a chronic disease that affects articular cartilage, causing its degeneration. Although OA is one of the most prevalent pathologies globally, there are no definitive treatments available. Recently, research has focused on elucidating the complex interplay that takes place between inflammatory processes and epigenetic regulation, showing that histone post-translational modifications (PTMs) can exert a pronounced effect on the expression of OA-related genes. OA chondrocytes enhance the production of interleukin 1β (IL-1β) and interleukin 8 (IL-8), which are epigenetically regulated. These cytokines upregulate the synthesis of matrix metalloproteinases (MMPs) and aggrecanases, which promote the extracellular matrix (ECM) destruction. This motivates the study of histone PTMs to investigate the epigenetic regulation of proinflammatory molecules, but the absence of specific protocols to extract histones from human articular cartilage has complicated this task. The lack of effective methods can be explained by the structural complexity and low cellularity of this tissue, which are responsible for the biomechanical properties that allow the movement of the joint but also complicate histone isolation. Here, we provide a histone extraction procedure specifically adapted for cryopreserved human articular cartilage that can be useful to understand epigenetic regulation in OA and accelerate the search for novel strategies.https://www.mdpi.com/1422-0067/23/6/3355histonechondrocytescartilagemethodsepigeneticsosteoarthritis |
| spellingShingle | Carmen Núñez-Carro Margarita Blanco-Blanco Tatiana Montoya Karla M. Villagrán-Andrade Tamara Hermida-Gómez Francisco J. Blanco María C. de Andrés Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis International Journal of Molecular Sciences histone chondrocytes cartilage methods epigenetics osteoarthritis |
| title | Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis |
| title_full | Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis |
| title_fullStr | Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis |
| title_full_unstemmed | Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis |
| title_short | Histone Extraction from Human Articular Cartilage for the Study of Epigenetic Regulation in Osteoarthritis |
| title_sort | histone extraction from human articular cartilage for the study of epigenetic regulation in osteoarthritis |
| topic | histone chondrocytes cartilage methods epigenetics osteoarthritis |
| url | https://www.mdpi.com/1422-0067/23/6/3355 |
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