An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration
Cell–hydrogel constructs are frequently used as injectable platforms for irregular cartilage regeneration. However, cell–hydrogel constructs have obvious disadvantages, such as long culture times, high probability of infection, and poor cartilage formation capacity, significantly limiting their clin...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2022.884036/full |
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| author | Wei Xu Wei Xu Wei Xu Tao Wang Tao Wang Tao Wang Yahui Wang Xiaodi Wu Xiaodi Wu Xiaodi Wu Yujie Chen Daiying Song Daiying Song Daiying Song Zheng Ci Yilin Cao Yilin Cao Yujie Hua Yujie Hua Guangdong Zhou Guangdong Zhou Guangdong Zhou Yu Liu Yu Liu Yu Liu Yu Liu |
| author_facet | Wei Xu Wei Xu Wei Xu Tao Wang Tao Wang Tao Wang Yahui Wang Xiaodi Wu Xiaodi Wu Xiaodi Wu Yujie Chen Daiying Song Daiying Song Daiying Song Zheng Ci Yilin Cao Yilin Cao Yujie Hua Yujie Hua Guangdong Zhou Guangdong Zhou Guangdong Zhou Yu Liu Yu Liu Yu Liu Yu Liu |
| author_sort | Wei Xu |
| collection | DOAJ |
| description | Cell–hydrogel constructs are frequently used as injectable platforms for irregular cartilage regeneration. However, cell–hydrogel constructs have obvious disadvantages, such as long culture times, high probability of infection, and poor cartilage formation capacity, significantly limiting their clinical translation. In this study, we aimed to develop a novel injectable platform comprising engineered cartilage gel (ECG) and gelatin methacrylate (GelMA) to improve cartilage regeneration. We first prepared an ECG by cutting the in vitro engineered cartilage sheet into pieces. The chondrocytes and ECG were evenly encapsulated into GelMA to form Cell-GelMA and ECG-GelMA constructs. The ECG-GelMA construct exhibited preferred gel characteristics and superior biocompatibility compared with the Cell-GelMA construct counterpart. After subcutaneous implantation in nude mice and goat, both gross views and histological evaluations showed that the ECG-GelMA construct achieved more homogenous, stable, and mature cartilage regeneration than the Cell-GelMA construct. Immunological evaluations showed that ECG-GelMA had a mitigatory immunologic reaction than the Cell-GelMA construct. Overall, the results suggest that the ECG-GelMA is a promising injectable platform for cartilage regeneration that may advance clinical translation. |
| first_indexed | 2024-12-12T23:11:55Z |
| format | Article |
| id | doaj.art-be9d66ce0c674fcaa388a5677a427c04 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-12T23:11:55Z |
| publishDate | 2022-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-be9d66ce0c674fcaa388a5677a427c042022-12-22T00:08:34ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852022-04-011010.3389/fbioe.2022.884036884036An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage RegenerationWei Xu0Wei Xu1Wei Xu2Tao Wang3Tao Wang4Tao Wang5Yahui Wang6Xiaodi Wu7Xiaodi Wu8Xiaodi Wu9Yujie Chen10Daiying Song11Daiying Song12Daiying Song13Zheng Ci14Yilin Cao15Yilin Cao16Yujie Hua17Yujie Hua18Guangdong Zhou19Guangdong Zhou20Guangdong Zhou21Yu Liu22Yu Liu23Yu Liu24Yu Liu25Research Institute of Plastic Surgery, Wei Fang Medical College, Weifang, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Stem Cell Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaResearch Institute of Plastic Surgery, Wei Fang Medical College, Weifang, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Stem Cell Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaResearch Institute of Plastic Surgery, Wei Fang Medical College, Weifang, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Stem Cell Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Engineering Research Center of Nano-Biomaterials and Regenerative Medicine, College of Chemistry, Chemical Engineering and Biotechnology, Donghua University, Shanghai, ChinaResearch Institute of Plastic Surgery, Wei Fang Medical College, Weifang, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Stem Cell Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Resthetic Bio CO., LTD, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Stem Cell Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Stem Cell Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaResearch Institute of Plastic Surgery, Wei Fang Medical College, Weifang, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Stem Cell Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaResearch Institute of Plastic Surgery, Wei Fang Medical College, Weifang, ChinaNational Tissue Engineering Center of China, Shanghai, ChinaShanghai Key Laboratory of Tissue Engineering, Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Stem Cell Institute, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Resthetic Bio CO., LTD, Shanghai, ChinaCell–hydrogel constructs are frequently used as injectable platforms for irregular cartilage regeneration. However, cell–hydrogel constructs have obvious disadvantages, such as long culture times, high probability of infection, and poor cartilage formation capacity, significantly limiting their clinical translation. In this study, we aimed to develop a novel injectable platform comprising engineered cartilage gel (ECG) and gelatin methacrylate (GelMA) to improve cartilage regeneration. We first prepared an ECG by cutting the in vitro engineered cartilage sheet into pieces. The chondrocytes and ECG were evenly encapsulated into GelMA to form Cell-GelMA and ECG-GelMA constructs. The ECG-GelMA construct exhibited preferred gel characteristics and superior biocompatibility compared with the Cell-GelMA construct counterpart. After subcutaneous implantation in nude mice and goat, both gross views and histological evaluations showed that the ECG-GelMA construct achieved more homogenous, stable, and mature cartilage regeneration than the Cell-GelMA construct. Immunological evaluations showed that ECG-GelMA had a mitigatory immunologic reaction than the Cell-GelMA construct. Overall, the results suggest that the ECG-GelMA is a promising injectable platform for cartilage regeneration that may advance clinical translation.https://www.frontiersin.org/articles/10.3389/fbioe.2022.884036/fullinjectable platformcell-hydrogelcartilage regenerationengineered cartilage gelimmune response |
| spellingShingle | Wei Xu Wei Xu Wei Xu Tao Wang Tao Wang Tao Wang Yahui Wang Xiaodi Wu Xiaodi Wu Xiaodi Wu Yujie Chen Daiying Song Daiying Song Daiying Song Zheng Ci Yilin Cao Yilin Cao Yujie Hua Yujie Hua Guangdong Zhou Guangdong Zhou Guangdong Zhou Yu Liu Yu Liu Yu Liu Yu Liu An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration Frontiers in Bioengineering and Biotechnology injectable platform cell-hydrogel cartilage regeneration engineered cartilage gel immune response |
| title | An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration |
| title_full | An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration |
| title_fullStr | An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration |
| title_full_unstemmed | An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration |
| title_short | An Injectable Platform of Engineered Cartilage Gel and Gelatin Methacrylate to Promote Cartilage Regeneration |
| title_sort | injectable platform of engineered cartilage gel and gelatin methacrylate to promote cartilage regeneration |
| topic | injectable platform cell-hydrogel cartilage regeneration engineered cartilage gel immune response |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2022.884036/full |
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