Effect of eight weeks of aerobic training and curcumin supplementation on PGC1-alpha gene expression and mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model
Background: Mitochondrial dysfunction is one of the possible mechanisms for the progression of heart disease. This study aimed to effect of eight weeks of aerobic training and curcumin supplementation on PGC1- α gene expression and cardiomyocyte mitochondrial citrate synthase in Cardiomyocytes of ma...
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| Format: | Article |
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Kashan University of Medical Sciences and Health Services
2022-10-01
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| Series: | Fiyz̤ |
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| Online Access: | http://feyz.kaums.ac.ir/article-1-4609-en.html |
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| author | Alireza Nikoozadeh Roghayeh Pouzesh-Jadidi Alireza Nourazar Masoud Asgharpour-Arshad Jabar Bashiri |
| author_facet | Alireza Nikoozadeh Roghayeh Pouzesh-Jadidi Alireza Nourazar Masoud Asgharpour-Arshad Jabar Bashiri |
| author_sort | Alireza Nikoozadeh |
| collection | DOAJ |
| description | Background: Mitochondrial dysfunction is one of the possible mechanisms for the progression of heart disease. This study aimed to effect of eight weeks of aerobic training and curcumin supplementation on PGC1- α gene expression and cardiomyocyte mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model.
Materials and Methods: 40 male rats were randomly divided into 5 groups: healthy control, heart myocardial infarction control, aerobic exercise, supplement and exercise + supplement. The training and training+supplement groups were influenced by training on the intelligent electronic animal treadmill for 8 weeks, 5 days a week. At this time, the myocardial infarction control and supplement groups had no exercise program. First, healthy controls were killed. Expression of PGC1-α gene and mitochondrial citrate synthase using real-time PCR method, the obtained data were analyzed using Shapiro-Wilk test, two-way and one-way analysis of variance and Tukey's post hoc test.
Result: Results showed that the expression of PGC1-α and citrate synthase of cardiomyocytes in the groups trained with and without curcumin consumption is significantly higher than the supplemented and myocardial infarction control groups (P=0.001). No significant difference was observed between the two groups of exercise and exercise + supplement. The expression of both genes in the myocardial infarction control group was lower than that of healthy controls (P≤0.05).
Conclusion: Eight weeks of aerobic exercise alone and in combination with curcumin increased the expression of PGC1-α gene and citrate synthase. The combined effect of exercise and curcumin were nonsignificantly greater. |
| first_indexed | 2024-04-13T18:41:07Z |
| format | Article |
| id | doaj.art-be9d765365864a4aaf79aeaeb64a64ec |
| institution | Directory Open Access Journal |
| issn | 1029-7855 2008-9821 |
| language | fas |
| last_indexed | 2024-04-13T18:41:07Z |
| publishDate | 2022-10-01 |
| publisher | Kashan University of Medical Sciences and Health Services |
| record_format | Article |
| series | Fiyz̤ |
| spelling | doaj.art-be9d765365864a4aaf79aeaeb64a64ec2022-12-22T02:34:44ZfasKashan University of Medical Sciences and Health ServicesFiyz̤1029-78552008-98212022-10-01264398406 Effect of eight weeks of aerobic training and curcumin supplementation on PGC1-alpha gene expression and mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model Alireza Nikoozadeh0Roghayeh Pouzesh-Jadidi 1Alireza Nourazar 2Masoud Asgharpour-Arshad 3Jabar Bashiri 4PHD student of Exercise Physiology, Department of Sport Sciences, Tabriz Branch, Islamic Azad University, Tabriz, I.R. Iran. Department of Sport Sciences, Tabriz Branch, Islamic Azad University, Tabriz, I.R. Iran.Department of Basic Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Tabriz, I.R. Iran. Department of Physical Education, Basic Sciences Education Center, Amin University of Law Enforcement Sciences, Tehran, I.R. Iran.Department of Sport Sciences, Tabriz Branch, Islamic Azad University, Tabriz, I.R. Iran.Background: Mitochondrial dysfunction is one of the possible mechanisms for the progression of heart disease. This study aimed to effect of eight weeks of aerobic training and curcumin supplementation on PGC1- α gene expression and cardiomyocyte mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model. Materials and Methods: 40 male rats were randomly divided into 5 groups: healthy control, heart myocardial infarction control, aerobic exercise, supplement and exercise + supplement. The training and training+supplement groups were influenced by training on the intelligent electronic animal treadmill for 8 weeks, 5 days a week. At this time, the myocardial infarction control and supplement groups had no exercise program. First, healthy controls were killed. Expression of PGC1-α gene and mitochondrial citrate synthase using real-time PCR method, the obtained data were analyzed using Shapiro-Wilk test, two-way and one-way analysis of variance and Tukey's post hoc test. Result: Results showed that the expression of PGC1-α and citrate synthase of cardiomyocytes in the groups trained with and without curcumin consumption is significantly higher than the supplemented and myocardial infarction control groups (P=0.001). No significant difference was observed between the two groups of exercise and exercise + supplement. The expression of both genes in the myocardial infarction control group was lower than that of healthy controls (P≤0.05). Conclusion: Eight weeks of aerobic exercise alone and in combination with curcumin increased the expression of PGC1-α gene and citrate synthase. The combined effect of exercise and curcumin were nonsignificantly greater.http://feyz.kaums.ac.ir/article-1-4609-en.htmlaerobic exercisecurcuminmyocardial infarctionperoxisome proliferator activated receptor alphapgc1-αcitrate synthase |
| spellingShingle | Alireza Nikoozadeh Roghayeh Pouzesh-Jadidi Alireza Nourazar Masoud Asgharpour-Arshad Jabar Bashiri Effect of eight weeks of aerobic training and curcumin supplementation on PGC1-alpha gene expression and mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model Fiyz̤ aerobic exercise curcumin myocardial infarction peroxisome proliferator activated receptor alpha pgc1-α citrate synthase |
| title | Effect of eight weeks of aerobic training and curcumin supplementation on PGC1-alpha gene expression and mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model |
| title_full | Effect of eight weeks of aerobic training and curcumin supplementation on PGC1-alpha gene expression and mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model |
| title_fullStr | Effect of eight weeks of aerobic training and curcumin supplementation on PGC1-alpha gene expression and mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model |
| title_full_unstemmed | Effect of eight weeks of aerobic training and curcumin supplementation on PGC1-alpha gene expression and mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model |
| title_short | Effect of eight weeks of aerobic training and curcumin supplementation on PGC1-alpha gene expression and mitochondrial citrate synthase in Cardiomyocytes of male rats with myocardial infarction model |
| title_sort | effect of eight weeks of aerobic training and curcumin supplementation on pgc1 alpha gene expression and mitochondrial citrate synthase in cardiomyocytes of male rats with myocardial infarction model |
| topic | aerobic exercise curcumin myocardial infarction peroxisome proliferator activated receptor alpha pgc1-α citrate synthase |
| url | http://feyz.kaums.ac.ir/article-1-4609-en.html |
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