Single-Molecule Detection of Nucleic Acids via Liposome Signal Amplification in Mass Spectrometry
A single-molecule detection method was developed for nucleic acids based on mass spectrometry counting single liposome particles. Before the appearance of symptoms, a negligible amount of nucleic acids and biomarkers for the clinical diagnosis of the disease were already present. However, it is diff...
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MDPI AG
2022-02-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/22/4/1346 |
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| author | Xiangcheng Lin Mengmeng Zhao Mingyue Li Juan Long Jing Zhang Fang Yu Fen Xu Lixian Sun |
| author_facet | Xiangcheng Lin Mengmeng Zhao Mingyue Li Juan Long Jing Zhang Fang Yu Fen Xu Lixian Sun |
| author_sort | Xiangcheng Lin |
| collection | DOAJ |
| description | A single-molecule detection method was developed for nucleic acids based on mass spectrometry counting single liposome particles. Before the appearance of symptoms, a negligible amount of nucleic acids and biomarkers for the clinical diagnosis of the disease were already present. However, it is difficult to detect extremely low concentrations of nucleic acids using the current methods. Hence, the establishment of an ultra-sensitive nucleic acid detection technique is urgently needed. Herein, magnetic beads were used to capture target nucleic acids, and liposome particles were employed as mass tags for single-particle measurements. Liposomes were released from magnetic beads via photocatalytic cleavage. Hence, one DNA molecule corresponded to one liposome particle, which could be counted using mass spectrometric measurement. The ultrasensitive detection of DNA (10<sup>–18</sup> M) was achieved using this method. |
| first_indexed | 2024-03-09T21:07:25Z |
| format | Article |
| id | doaj.art-be9fa25938ba45718e77d80d25040897 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T21:07:25Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
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| series | Sensors |
| spelling | doaj.art-be9fa25938ba45718e77d80d250408972023-11-23T21:58:06ZengMDPI AGSensors1424-82202022-02-01224134610.3390/s22041346Single-Molecule Detection of Nucleic Acids via Liposome Signal Amplification in Mass SpectrometryXiangcheng Lin0Mengmeng Zhao1Mingyue Li2Juan Long3Jing Zhang4Fang Yu5Fen Xu6Lixian Sun7Guangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, ChinaGuangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, ChinaGuangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, ChinaGuangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, ChinaGuangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, ChinaGuangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, ChinaGuangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, ChinaGuangxi Key Laboratory of Information Materials, School of Materials Science and Engineering, Guilin University of Electronic Technology, Guilin 541004, ChinaA single-molecule detection method was developed for nucleic acids based on mass spectrometry counting single liposome particles. Before the appearance of symptoms, a negligible amount of nucleic acids and biomarkers for the clinical diagnosis of the disease were already present. However, it is difficult to detect extremely low concentrations of nucleic acids using the current methods. Hence, the establishment of an ultra-sensitive nucleic acid detection technique is urgently needed. Herein, magnetic beads were used to capture target nucleic acids, and liposome particles were employed as mass tags for single-particle measurements. Liposomes were released from magnetic beads via photocatalytic cleavage. Hence, one DNA molecule corresponded to one liposome particle, which could be counted using mass spectrometric measurement. The ultrasensitive detection of DNA (10<sup>–18</sup> M) was achieved using this method.https://www.mdpi.com/1424-8220/22/4/1346mass spectrumnucleic acidsignal amplificationliposome |
| spellingShingle | Xiangcheng Lin Mengmeng Zhao Mingyue Li Juan Long Jing Zhang Fang Yu Fen Xu Lixian Sun Single-Molecule Detection of Nucleic Acids via Liposome Signal Amplification in Mass Spectrometry Sensors mass spectrum nucleic acid signal amplification liposome |
| title | Single-Molecule Detection of Nucleic Acids via Liposome Signal Amplification in Mass Spectrometry |
| title_full | Single-Molecule Detection of Nucleic Acids via Liposome Signal Amplification in Mass Spectrometry |
| title_fullStr | Single-Molecule Detection of Nucleic Acids via Liposome Signal Amplification in Mass Spectrometry |
| title_full_unstemmed | Single-Molecule Detection of Nucleic Acids via Liposome Signal Amplification in Mass Spectrometry |
| title_short | Single-Molecule Detection of Nucleic Acids via Liposome Signal Amplification in Mass Spectrometry |
| title_sort | single molecule detection of nucleic acids via liposome signal amplification in mass spectrometry |
| topic | mass spectrum nucleic acid signal amplification liposome |
| url | https://www.mdpi.com/1424-8220/22/4/1346 |
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