Vitamin D and vitamin D receptor in patients with ophthalmic pterygium
Pterygium, an ultraviolet radiation (UV)-related disease, is a relatively benign process, but since it displays tumor-like features, it has been proposed to be a neoplastic- like growth disorder. Vitamin D performs a number of functions in addition to calcium homeostasis, as inhibition of cell proli...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
PAGEPress Publications
2017-10-01
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| Series: | European Journal of Histochemistry |
| Subjects: | |
| Online Access: | http://ejh.it/index.php/ejh/article/view/2837 |
| _version_ | 1811264784207708160 |
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| author | Cristina Maxia Daniela Murtas Michela Corrias Ignazio Zucca Luigi Minerba Franca Piras Cristiana Marinelli Maria Teresa Perra |
| author_facet | Cristina Maxia Daniela Murtas Michela Corrias Ignazio Zucca Luigi Minerba Franca Piras Cristiana Marinelli Maria Teresa Perra |
| author_sort | Cristina Maxia |
| collection | DOAJ |
| description | Pterygium, an ultraviolet radiation (UV)-related disease, is a relatively benign process, but since it displays tumor-like features, it has been proposed to be a neoplastic- like growth disorder. Vitamin D performs a number of functions in addition to calcium homeostasis, as inhibition of cell proliferation, activation of apoptotic pathways, and inhibition of angiogenesis. Since the antitumor actions of vitamin D are mediated primarily through the nuclear vitamin D receptor (VDR), the aim of the present study was to investigate vitamin D status in patients with pterygium and in control subjects, and VDR immunohistochemical expression in samples of pterygium and normal conjunctiva in order to evaluate a possible role of vitamin D pathway in the pathogenesis of the disease. Serum vitamin D concentration was measured among 41 patients with pterygium and 47 volunteers by an automated chemiluminescence immunoassay. Moreover, 23 formalin- fixed and paraffin-embedded pterygium biopsy samples and 24 conjunctiva specimens were treated for the immunohistochemical demonstration of VDR using the streptavidin-biotin alkaline phosphatase method. No differences were observed about vitamin D level between patient with pterygium and control group, but significant differences between VDR immunolocalization in pterygium and normal conjunctiva were observed (P=0.00001). In conjunctiva, the immunoreactivity, localized mainly in cytoplasm of epithelial cells, may probably demonstrate VDR regulation of cell growth, differentiation, and apoptosis, while in pterygium VDR co-localization in the nucleus and cytoplasm of epithelial cells may indicate alternative nuclear pathways by which vitamin D might exert its antiinflammatory and anti-proliferative effects by the regulation of gene expression. |
| first_indexed | 2024-04-12T20:10:34Z |
| format | Article |
| id | doaj.art-be9fab2574434728b0b3dc2a4bd0a6c9 |
| institution | Directory Open Access Journal |
| issn | 1121-760X 2038-8306 |
| language | English |
| last_indexed | 2024-04-12T20:10:34Z |
| publishDate | 2017-10-01 |
| publisher | PAGEPress Publications |
| record_format | Article |
| series | European Journal of Histochemistry |
| spelling | doaj.art-be9fab2574434728b0b3dc2a4bd0a6c92022-12-22T03:18:16ZengPAGEPress PublicationsEuropean Journal of Histochemistry1121-760X2038-83062017-10-0161410.4081/ejh.2017.28371582Vitamin D and vitamin D receptor in patients with ophthalmic pterygiumCristina Maxia0Daniela Murtas1Michela Corrias2Ignazio Zucca3Luigi Minerba4Franca Piras5Cristiana Marinelli6Maria Teresa Perra7University of Cagliari, Department of Biomedical SciencesUniversity of Cagliari, Department of Biomedical SciencesUniversity of Cagliari, Department of Biomedical SciencesUniversity of Cagliari, Department of Surgical ScienceUniversity of Cagliari, Department of Public Health, Clinical and Molecular MedicineUniversity of Cagliari, Department of Biomedical SciencesHospital "G. Brotzu", Laboratory of Clinical Chemistry, Hematology and MicrobiologyUniversity of Cagliari, Department of Biomedical SciencesPterygium, an ultraviolet radiation (UV)-related disease, is a relatively benign process, but since it displays tumor-like features, it has been proposed to be a neoplastic- like growth disorder. Vitamin D performs a number of functions in addition to calcium homeostasis, as inhibition of cell proliferation, activation of apoptotic pathways, and inhibition of angiogenesis. Since the antitumor actions of vitamin D are mediated primarily through the nuclear vitamin D receptor (VDR), the aim of the present study was to investigate vitamin D status in patients with pterygium and in control subjects, and VDR immunohistochemical expression in samples of pterygium and normal conjunctiva in order to evaluate a possible role of vitamin D pathway in the pathogenesis of the disease. Serum vitamin D concentration was measured among 41 patients with pterygium and 47 volunteers by an automated chemiluminescence immunoassay. Moreover, 23 formalin- fixed and paraffin-embedded pterygium biopsy samples and 24 conjunctiva specimens were treated for the immunohistochemical demonstration of VDR using the streptavidin-biotin alkaline phosphatase method. No differences were observed about vitamin D level between patient with pterygium and control group, but significant differences between VDR immunolocalization in pterygium and normal conjunctiva were observed (P=0.00001). In conjunctiva, the immunoreactivity, localized mainly in cytoplasm of epithelial cells, may probably demonstrate VDR regulation of cell growth, differentiation, and apoptosis, while in pterygium VDR co-localization in the nucleus and cytoplasm of epithelial cells may indicate alternative nuclear pathways by which vitamin D might exert its antiinflammatory and anti-proliferative effects by the regulation of gene expression.http://ejh.it/index.php/ejh/article/view/2837Pterygiumconjunctivavitamin Dvitamin D receptor (VDR)immunohistochemistry. |
| spellingShingle | Cristina Maxia Daniela Murtas Michela Corrias Ignazio Zucca Luigi Minerba Franca Piras Cristiana Marinelli Maria Teresa Perra Vitamin D and vitamin D receptor in patients with ophthalmic pterygium European Journal of Histochemistry Pterygium conjunctiva vitamin D vitamin D receptor (VDR) immunohistochemistry. |
| title | Vitamin D and vitamin D receptor in patients with ophthalmic pterygium |
| title_full | Vitamin D and vitamin D receptor in patients with ophthalmic pterygium |
| title_fullStr | Vitamin D and vitamin D receptor in patients with ophthalmic pterygium |
| title_full_unstemmed | Vitamin D and vitamin D receptor in patients with ophthalmic pterygium |
| title_short | Vitamin D and vitamin D receptor in patients with ophthalmic pterygium |
| title_sort | vitamin d and vitamin d receptor in patients with ophthalmic pterygium |
| topic | Pterygium conjunctiva vitamin D vitamin D receptor (VDR) immunohistochemistry. |
| url | http://ejh.it/index.php/ejh/article/view/2837 |
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