GhWDL3 is involved in the formation and development of fiber cell morphology in upland cotton (Gossypium hirsutum L.)
Abstract Background Cotton fiber is a model tissue for studying microtubule-associated proteins (MAPs). The Xklp2 (TPX2) proteins that belong to the novel MAPs member mainly participate in the formation and development of microtubule (MT). However, there is a lack of studies concerning the systemati...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-02-01
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| Series: | Journal of Cotton Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s42397-024-00167-0 |
| _version_ | 1797276014236139520 |
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| author | Baojun Chen Zailong Tian Guoyong Fu Ai Zhang Yaru Sun Jingjing Wang Zhaoe Pan Hongge Li Daowu Hu Yingying Xia Shoupu He Xiongming Du |
| author_facet | Baojun Chen Zailong Tian Guoyong Fu Ai Zhang Yaru Sun Jingjing Wang Zhaoe Pan Hongge Li Daowu Hu Yingying Xia Shoupu He Xiongming Du |
| author_sort | Baojun Chen |
| collection | DOAJ |
| description | Abstract Background Cotton fiber is a model tissue for studying microtubule-associated proteins (MAPs). The Xklp2 (TPX2) proteins that belong to the novel MAPs member mainly participate in the formation and development of microtubule (MT). However, there is a lack of studies concerning the systematic characterization of the TPX2 genes family in cotton. Therefore, the identification and portrayal of G. hirsutum TPX2 genes can provide key targets for molecular manipulation in the breeding of cotton fiber improvement. Result In this study, TPX2 family genes were classified into two distinct subclasses TPXLs and MAP genes WAVE DAMPENED2-LIKE (WDLs) and quite conservative in quantity. GhWDL3 was significantly up-regulated in 15 days post anthesis fibers of ZRI-015 (an upland cotton with longer and stronger fiber). GhWDL3 promotes all stem hairs to become straight when overexpressed in Arabidopsis, which may indirectly regulate cotton fiber cell morphology during fiber development. Virus induced gene silencing (VIGS) results showed that GhWDL3 inhibited fiber cell elongation at fiber development periods through regulating the expression of cell wall related genes. Conclusion These results reveal that GhWDL3 regulated cotton fiber cell elongation and provide crucial information for the further investigation in the regulatory mechanisms/networks of cotton fiber length. |
| first_indexed | 2024-03-07T15:22:15Z |
| format | Article |
| id | doaj.art-bea0d0d2e0a448c18eecc23dfe2c385f |
| institution | Directory Open Access Journal |
| issn | 2523-3254 |
| language | English |
| last_indexed | 2024-03-07T15:22:15Z |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Cotton Research |
| spelling | doaj.art-bea0d0d2e0a448c18eecc23dfe2c385f2024-03-05T17:34:50ZengBMCJournal of Cotton Research2523-32542024-02-017111110.1186/s42397-024-00167-0GhWDL3 is involved in the formation and development of fiber cell morphology in upland cotton (Gossypium hirsutum L.)Baojun Chen0Zailong Tian1Guoyong Fu2Ai Zhang3Yaru Sun4Jingjing Wang5Zhaoe Pan6Hongge Li7Daowu Hu8Yingying Xia9Shoupu He10Xiongming Du11Zhengzhou Research Base, National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Zhengzhou UniversityNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural SciencesNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural SciencesNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural SciencesNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural SciencesNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural SciencesNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural SciencesZhengzhou Research Base, National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Zhengzhou UniversityNational Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Institute of Cotton Research, Chinese Academy of Agricultural SciencesNational Supercomputing Center in Zhengzhou, Zhengzhou UniversityZhengzhou Research Base, National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Zhengzhou UniversityZhengzhou Research Base, National Key Laboratory of Cotton Bio-Breeding and Integrated Utilization, Zhengzhou UniversityAbstract Background Cotton fiber is a model tissue for studying microtubule-associated proteins (MAPs). The Xklp2 (TPX2) proteins that belong to the novel MAPs member mainly participate in the formation and development of microtubule (MT). However, there is a lack of studies concerning the systematic characterization of the TPX2 genes family in cotton. Therefore, the identification and portrayal of G. hirsutum TPX2 genes can provide key targets for molecular manipulation in the breeding of cotton fiber improvement. Result In this study, TPX2 family genes were classified into two distinct subclasses TPXLs and MAP genes WAVE DAMPENED2-LIKE (WDLs) and quite conservative in quantity. GhWDL3 was significantly up-regulated in 15 days post anthesis fibers of ZRI-015 (an upland cotton with longer and stronger fiber). GhWDL3 promotes all stem hairs to become straight when overexpressed in Arabidopsis, which may indirectly regulate cotton fiber cell morphology during fiber development. Virus induced gene silencing (VIGS) results showed that GhWDL3 inhibited fiber cell elongation at fiber development periods through regulating the expression of cell wall related genes. Conclusion These results reveal that GhWDL3 regulated cotton fiber cell elongation and provide crucial information for the further investigation in the regulatory mechanisms/networks of cotton fiber length.https://doi.org/10.1186/s42397-024-00167-0Upland cottonGhWDL3Fiber lengthTPX2CytoskeletonMicrotubule-associated proteins (MAPs) |
| spellingShingle | Baojun Chen Zailong Tian Guoyong Fu Ai Zhang Yaru Sun Jingjing Wang Zhaoe Pan Hongge Li Daowu Hu Yingying Xia Shoupu He Xiongming Du GhWDL3 is involved in the formation and development of fiber cell morphology in upland cotton (Gossypium hirsutum L.) Journal of Cotton Research Upland cotton GhWDL3 Fiber length TPX2 Cytoskeleton Microtubule-associated proteins (MAPs) |
| title | GhWDL3 is involved in the formation and development of fiber cell morphology in upland cotton (Gossypium hirsutum L.) |
| title_full | GhWDL3 is involved in the formation and development of fiber cell morphology in upland cotton (Gossypium hirsutum L.) |
| title_fullStr | GhWDL3 is involved in the formation and development of fiber cell morphology in upland cotton (Gossypium hirsutum L.) |
| title_full_unstemmed | GhWDL3 is involved in the formation and development of fiber cell morphology in upland cotton (Gossypium hirsutum L.) |
| title_short | GhWDL3 is involved in the formation and development of fiber cell morphology in upland cotton (Gossypium hirsutum L.) |
| title_sort | ghwdl3 is involved in the formation and development of fiber cell morphology in upland cotton gossypium hirsutum l |
| topic | Upland cotton GhWDL3 Fiber length TPX2 Cytoskeleton Microtubule-associated proteins (MAPs) |
| url | https://doi.org/10.1186/s42397-024-00167-0 |
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