Influence of the At-Arrival Host Transcriptome on Bovine Respiratory Disease Incidence during Backgrounding
Bovine respiratory disease (BRD) remains the leading disease within the U.S. beef cattle industry. Marketing decisions made prior to backgrounding may shift BRD incidence into a different phase of production, and the importance of host gene expression on BRD incidence as it relates to marketing stra...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/10/3/211 |
| _version_ | 1827747369016360960 |
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| author | Mollie M. Green Amelia R. Woolums Brandi B. Karisch Kelsey M. Harvey Sarah F. Capik Matthew A. Scott |
| author_facet | Mollie M. Green Amelia R. Woolums Brandi B. Karisch Kelsey M. Harvey Sarah F. Capik Matthew A. Scott |
| author_sort | Mollie M. Green |
| collection | DOAJ |
| description | Bovine respiratory disease (BRD) remains the leading disease within the U.S. beef cattle industry. Marketing decisions made prior to backgrounding may shift BRD incidence into a different phase of production, and the importance of host gene expression on BRD incidence as it relates to marketing strategy is poorly understood. Our objective was to compare the influence of marketing on host transcriptomes measured on arrival at a backgrounding facility on the subsequent probability of being treated for BRD during a 45-day backgrounding phase. This study, through RNA-Seq analysis of blood samples collected on arrival, evaluated gene expression differences between cattle which experienced a commercial auction setting (AUCTION) versus cattle directly shipped to backgrounding from the cow–calf phase (DIRECT); further analyses were conducted to determine differentially expressed genes (DEGs) between cattle which remained clinically healthy during backgrounding (HEALTHY) versus those that required treatment for clinical BRD within 45 days of arrival (BRD). A profound difference in DEGs (n = 2961) was identified between AUCTION cattle compared to DIRECT cattle, regardless of BRD development; these DEGs encoded for proteins involved in antiviral defense (increased in AUCTION), cell growth regulation (decreased in AUCTION), and inflammatory mediation (decreased in AUCTION). Nine and four DEGs were identified between BRD and HEALTHY cohorts in the AUCTION and DIRECT groups, respectively; DEGs between disease cohorts in the AUCTION group encoded for proteins involved in collagen synthesis and platelet aggregation (increased in HEALTHY). Our work demonstrates the clear influence marketing has on host expression and identified genes and mechanisms which may predict BRD risk. |
| first_indexed | 2024-03-11T05:47:28Z |
| format | Article |
| id | doaj.art-bea11526a5bc40d8a6dc45761f42050d |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-11T05:47:28Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-bea11526a5bc40d8a6dc45761f42050d2023-11-17T14:20:51ZengMDPI AGVeterinary Sciences2306-73812023-03-0110321110.3390/vetsci10030211Influence of the At-Arrival Host Transcriptome on Bovine Respiratory Disease Incidence during BackgroundingMollie M. Green0Amelia R. Woolums1Brandi B. Karisch2Kelsey M. Harvey3Sarah F. Capik4Matthew A. Scott5Department of Agricultural Sciences, West Texas A&M University, Canyon, TX 79016, USADepartment of Pathobiology and Population Medicine, Mississippi State University, Mississippi State, MS 39762, USADepartment of Animal and Dairy Sciences, Mississippi State University, Starkville, MS 39762, USAPrairie Research Unit, Mississippi State University, Prairie, MS 39756, USATumbleweed Veterinary Services, PLLC, Amarillo, TX 79159, USAVeterinary Education, Research, and Outreach Center, Texas A&M University, Canyon, TX 79016, USABovine respiratory disease (BRD) remains the leading disease within the U.S. beef cattle industry. Marketing decisions made prior to backgrounding may shift BRD incidence into a different phase of production, and the importance of host gene expression on BRD incidence as it relates to marketing strategy is poorly understood. Our objective was to compare the influence of marketing on host transcriptomes measured on arrival at a backgrounding facility on the subsequent probability of being treated for BRD during a 45-day backgrounding phase. This study, through RNA-Seq analysis of blood samples collected on arrival, evaluated gene expression differences between cattle which experienced a commercial auction setting (AUCTION) versus cattle directly shipped to backgrounding from the cow–calf phase (DIRECT); further analyses were conducted to determine differentially expressed genes (DEGs) between cattle which remained clinically healthy during backgrounding (HEALTHY) versus those that required treatment for clinical BRD within 45 days of arrival (BRD). A profound difference in DEGs (n = 2961) was identified between AUCTION cattle compared to DIRECT cattle, regardless of BRD development; these DEGs encoded for proteins involved in antiviral defense (increased in AUCTION), cell growth regulation (decreased in AUCTION), and inflammatory mediation (decreased in AUCTION). Nine and four DEGs were identified between BRD and HEALTHY cohorts in the AUCTION and DIRECT groups, respectively; DEGs between disease cohorts in the AUCTION group encoded for proteins involved in collagen synthesis and platelet aggregation (increased in HEALTHY). Our work demonstrates the clear influence marketing has on host expression and identified genes and mechanisms which may predict BRD risk.https://www.mdpi.com/2306-7381/10/3/211bovine respiratory diseasecattlecollagencytokinemanagementmarketing |
| spellingShingle | Mollie M. Green Amelia R. Woolums Brandi B. Karisch Kelsey M. Harvey Sarah F. Capik Matthew A. Scott Influence of the At-Arrival Host Transcriptome on Bovine Respiratory Disease Incidence during Backgrounding Veterinary Sciences bovine respiratory disease cattle collagen cytokine management marketing |
| title | Influence of the At-Arrival Host Transcriptome on Bovine Respiratory Disease Incidence during Backgrounding |
| title_full | Influence of the At-Arrival Host Transcriptome on Bovine Respiratory Disease Incidence during Backgrounding |
| title_fullStr | Influence of the At-Arrival Host Transcriptome on Bovine Respiratory Disease Incidence during Backgrounding |
| title_full_unstemmed | Influence of the At-Arrival Host Transcriptome on Bovine Respiratory Disease Incidence during Backgrounding |
| title_short | Influence of the At-Arrival Host Transcriptome on Bovine Respiratory Disease Incidence during Backgrounding |
| title_sort | influence of the at arrival host transcriptome on bovine respiratory disease incidence during backgrounding |
| topic | bovine respiratory disease cattle collagen cytokine management marketing |
| url | https://www.mdpi.com/2306-7381/10/3/211 |
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