| Summary: | The pollution of industrial wastewater has become a global issue in terms of economic development and ecological protection. <i>Pseudomonas oleovorans</i> has been studied as a bacterium involved in the treatment of petroleum pollutants. Our study aimed to investigate the physicochemical properties and drug resistance of <i>Pseudomonas oleovorans</i> isolated from industrial wastewater with a high concentration of sulfate compounds. Firstly, <i>Pseudomonas oleovorans</i> was isolated and then identified using matrix-assisted flight mass spectrometry and 16S rDNA sequencing. Then, biochemical and antibiotic resistance analyses were performed on the <i>Pseudomonas oleovorans</i>, and a microbial high-throughput growth detector was used to assess the growth of the strain. Finally, PCR and proteomics analyses were conducted to determine drug-resistance-related genes/proteins. Based on the results of the spectrum diagram and sequencing, the isolated bacteria were identified as <i>Pseudomonas oleovorans</i> and were positive to reactions of ADH, MTE, CIT, MLT, ONPG, and ACE. <i>Pseudomonas oleovorans</i> was sensitive to most of the tested antibiotics, and its resistance to SXT and CHL and MIN and TIM was intermediate. The growth experiment showed that <i>Pseudomonas oleovorans</i> had a good growth rate in nutrient broth. Additionally, <i>gyrB</i> was the resistance gene, and mdtA2, mdtA3, mdtB2, mdaB, and emrK1 were the proteins that were closely associated with the drug resistance of <i>Pseudomonas oleovorans</i>. Our results show the biochemical properties of <i>Pseudomonas oleovorans</i> from industrial wastewater with a high concentration of sulfate compounds and provide a new perspective for <i>Pseudomonas oleovorans</i> to participate in biological removal of chemical pollutants in industrial wastewater.
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