Dryopteris dilatata extract alleviates neuronal derangements and kidney disturbances in streptozotocin-induced diabetic nephropathy via concomitant activation of Nrf2/Bcl-2/antioxidants axis
Background: Finding a possible therapeutic approach that could be used to manage or treat diabetes mellitus complications is consistently drawing public health attention. Meanwhile, several experimental studies have been geared toward elucidating the mechanisms underpinning diabetes and its comorbid...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Phytomedicine Plus |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031322001427 |
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| author | Ajirioghene E. Akpotu Jerome Ndudi Asiwe Aikpitanyi Ikponmwosa Jude Ijuo Abeje Harrison Ogheneochuko Eruotor Olusegun G. Adebayo |
| author_facet | Ajirioghene E. Akpotu Jerome Ndudi Asiwe Aikpitanyi Ikponmwosa Jude Ijuo Abeje Harrison Ogheneochuko Eruotor Olusegun G. Adebayo |
| author_sort | Ajirioghene E. Akpotu |
| collection | DOAJ |
| description | Background: Finding a possible therapeutic approach that could be used to manage or treat diabetes mellitus complications is consistently drawing public health attention. Meanwhile, several experimental studies have been geared toward elucidating the mechanisms underpinning diabetes and its comorbidities. This study was designed to investigate the therapeutic potential of methanol extract of Dryopteris dilatata (MEDD) in preventing neuronal derangements associated with streptozotocin-induced diabetic nephropathy in male Wistar rats. Methods: Twenty-five adult male Wistar rats (140–170) g were used for the study. Diabetes was induced using streptozotocin (60 mg/kg) followed by oral treatment of MEDD (400 and 800 mg/kg) and pioglitazone (10 mg/kg) for two weeks. Thereafter, further analyses were done. Results: The result showed that MEDD treatment positively modulates brain pro-oxidant activity (malonaldehyde and nitrite levels) and endogenous antioxidant (glutathione) and neurochemical release in the diabetic rats. The kidney pro-oxidants, endogenous antioxidants and pro-inflammatory mediators, creatinine, bilirubin, urea and electrolyte alterations and imbalances were also attenuated and regulated following MEDD treatment. Additionally, MEDD significantly upregulated the expression of Nfr2 and Bcl-2 proteins with decreased kidney injury scores in diabetic rats. Conclusion: We suggest that methanol extract of Dryopteris dilatata may be considered as a potential phytotherapeutic candidate in combating and managing diabetes and its complications. |
| first_indexed | 2024-04-13T11:35:36Z |
| format | Article |
| id | doaj.art-bea4933b02274dddb0001d0dfd3c8540 |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-04-13T11:35:36Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-bea4933b02274dddb0001d0dfd3c85402022-12-22T02:48:28ZengElsevierPhytomedicine Plus2667-03132022-11-0124100363Dryopteris dilatata extract alleviates neuronal derangements and kidney disturbances in streptozotocin-induced diabetic nephropathy via concomitant activation of Nrf2/Bcl-2/antioxidants axisAjirioghene E. Akpotu0Jerome Ndudi Asiwe1Aikpitanyi Ikponmwosa2Jude Ijuo Abeje3Harrison Ogheneochuko Eruotor4Olusegun G. Adebayo5Department of Pharmacology, Faculty of Basic Medical Sciences, PAMO University of Medical Sciences, Port-Harcourt, River State, NigeriaCardiorespiratory Unit, Department of Physiology, Faculty of Basic Medical Sciences, PAMO University of Medical Sciences, Port-Harcourt, River State, NigeriaDepartment of Physiology, School of Basic Medical Sciences, Igbinedion University, Okada, Edo State, NigeriaNeurosciences and Oral Physiology Unit, Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, NigeriaDepartment of Biochemistry, Faculty of Basic Medical Sciences, PAMO University of Medical Sciences, Port-Harcourt, River State, NigeriaNeurosciences and Oral Physiology Unit, Department of Physiology, Faculty of Basic Medical Sciences, College of Medicine, University of Ibadan, Ibadan, Nigeria; Neurophysiology Unit, Department of Physiology, Faculty of Basic Medical Sciences, PAMO University of Medical Sciences, Port-Harcourt, River State, Nigeria; Corresponding author at: Neurophysiology Unit, Department of Physiology, Faculty of Basic Medical Sciences, PAMO University of Medical Sciences, Port-Harcourt, River State, Nigeria.Background: Finding a possible therapeutic approach that could be used to manage or treat diabetes mellitus complications is consistently drawing public health attention. Meanwhile, several experimental studies have been geared toward elucidating the mechanisms underpinning diabetes and its comorbidities. This study was designed to investigate the therapeutic potential of methanol extract of Dryopteris dilatata (MEDD) in preventing neuronal derangements associated with streptozotocin-induced diabetic nephropathy in male Wistar rats. Methods: Twenty-five adult male Wistar rats (140–170) g were used for the study. Diabetes was induced using streptozotocin (60 mg/kg) followed by oral treatment of MEDD (400 and 800 mg/kg) and pioglitazone (10 mg/kg) for two weeks. Thereafter, further analyses were done. Results: The result showed that MEDD treatment positively modulates brain pro-oxidant activity (malonaldehyde and nitrite levels) and endogenous antioxidant (glutathione) and neurochemical release in the diabetic rats. The kidney pro-oxidants, endogenous antioxidants and pro-inflammatory mediators, creatinine, bilirubin, urea and electrolyte alterations and imbalances were also attenuated and regulated following MEDD treatment. Additionally, MEDD significantly upregulated the expression of Nfr2 and Bcl-2 proteins with decreased kidney injury scores in diabetic rats. Conclusion: We suggest that methanol extract of Dryopteris dilatata may be considered as a potential phytotherapeutic candidate in combating and managing diabetes and its complications.http://www.sciencedirect.com/science/article/pii/S2667031322001427DiabetesNephropathyNeuropathyDryopteris dilatataTranscription factor |
| spellingShingle | Ajirioghene E. Akpotu Jerome Ndudi Asiwe Aikpitanyi Ikponmwosa Jude Ijuo Abeje Harrison Ogheneochuko Eruotor Olusegun G. Adebayo Dryopteris dilatata extract alleviates neuronal derangements and kidney disturbances in streptozotocin-induced diabetic nephropathy via concomitant activation of Nrf2/Bcl-2/antioxidants axis Phytomedicine Plus Diabetes Nephropathy Neuropathy Dryopteris dilatata Transcription factor |
| title | Dryopteris dilatata extract alleviates neuronal derangements and kidney disturbances in streptozotocin-induced diabetic nephropathy via concomitant activation of Nrf2/Bcl-2/antioxidants axis |
| title_full | Dryopteris dilatata extract alleviates neuronal derangements and kidney disturbances in streptozotocin-induced diabetic nephropathy via concomitant activation of Nrf2/Bcl-2/antioxidants axis |
| title_fullStr | Dryopteris dilatata extract alleviates neuronal derangements and kidney disturbances in streptozotocin-induced diabetic nephropathy via concomitant activation of Nrf2/Bcl-2/antioxidants axis |
| title_full_unstemmed | Dryopteris dilatata extract alleviates neuronal derangements and kidney disturbances in streptozotocin-induced diabetic nephropathy via concomitant activation of Nrf2/Bcl-2/antioxidants axis |
| title_short | Dryopteris dilatata extract alleviates neuronal derangements and kidney disturbances in streptozotocin-induced diabetic nephropathy via concomitant activation of Nrf2/Bcl-2/antioxidants axis |
| title_sort | dryopteris dilatata extract alleviates neuronal derangements and kidney disturbances in streptozotocin induced diabetic nephropathy via concomitant activation of nrf2 bcl 2 antioxidants axis |
| topic | Diabetes Nephropathy Neuropathy Dryopteris dilatata Transcription factor |
| url | http://www.sciencedirect.com/science/article/pii/S2667031322001427 |
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