Partial sequencing of a putative Alstroemeria necrotic streak orthotospovirus isolate detected on lettuce in Colombia
Lettuce is the most cultivated leafy salad vegetable in Colombia, being the municipality of Madrid, in the Department of Cundinamarca, the second largest producer. In this region, lettuce plants with foliar symptoms characterized by brown necrotic spots forming an extended n...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Goiás
2021-08-01
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| Series: | Pesquisa Agropecuária Tropical |
| Subjects: | |
| Online Access: | https://www.revistas.ufg.br/pat/article/view/68585/37033 |
| _version_ | 1818908067089088512 |
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| author | Diego Alejandro Jurado-Rincón Linda Jeimmy Rincón-Rivera Angela María Vargas-Berdugo Adriana González-Almario |
| author_facet | Diego Alejandro Jurado-Rincón Linda Jeimmy Rincón-Rivera Angela María Vargas-Berdugo Adriana González-Almario |
| author_sort | Diego Alejandro Jurado-Rincón |
| collection | DOAJ |
| description | Lettuce is the most cultivated leafy salad vegetable in Colombia, being the municipality of Madrid, in the Department of Cundinamarca, the second largest producer. In this region, lettuce plants with foliar symptoms characterized by brown necrotic spots forming an extended necrotic area, chlorosis, leaf distortion and plant stunting have been detected, possibly caused by a viral infection associated with the Orthotospovirus genus. This study aimed to identify the orthotospovirus species associated with those symptoms, contributing to updating the lettuce phytosanitary status in this region. The presence of orthotospovirus was confirmed by the enzyme-linked immunosorbent assay (DAS-ELISA), although the sequence of the nucleocapsid (N) gene confirmed the presence of Alstroemeria necrotic streak orthotospovirus, disregarding the Tomato spotted wilt orthotospovirus and Impatiens necrotic spot orthotospovirus previously reported for this crop, being this its first report in lettuce crops in Colombia. |
| first_indexed | 2024-12-19T22:05:07Z |
| format | Article |
| id | doaj.art-bea5202c123d4aeb937ea5fbd845a702 |
| institution | Directory Open Access Journal |
| issn | 1983-4063 |
| language | English |
| last_indexed | 2024-12-19T22:05:07Z |
| publishDate | 2021-08-01 |
| publisher | Universidade Federal de Goiás |
| record_format | Article |
| series | Pesquisa Agropecuária Tropical |
| spelling | doaj.art-bea5202c123d4aeb937ea5fbd845a7022022-12-21T20:04:03ZengUniversidade Federal de GoiásPesquisa Agropecuária Tropical1983-40632021-08-0151e6858510.1590/1983-40632021v5168585Partial sequencing of a putative Alstroemeria necrotic streak orthotospovirus isolate detected on lettuce in ColombiaDiego Alejandro Jurado-Rincón0https://orcid.org/0000-0002-6183-0097Linda Jeimmy Rincón-Rivera1https://orcid.org/0000-0003-0495-9879Angela María Vargas-Berdugo2https://orcid.org/0000-0003-0671-3055Adriana González-Almario3https://orcid.org/0000-0003-2261-8912Universidad Nacional de Colombia, Facultad de Ciencias Agrarias, Bogotá, Colombia.Universidad Nacional de Colombia, Facultad de Ciencias Agrarias, Bogotá, Colombia.Agrosavia, Ibagué, Colombia.Universidad Nacional de Colombia, Facultad de Ciencias Agrarias, Bogotá, Colombia.Lettuce is the most cultivated leafy salad vegetable in Colombia, being the municipality of Madrid, in the Department of Cundinamarca, the second largest producer. In this region, lettuce plants with foliar symptoms characterized by brown necrotic spots forming an extended necrotic area, chlorosis, leaf distortion and plant stunting have been detected, possibly caused by a viral infection associated with the Orthotospovirus genus. This study aimed to identify the orthotospovirus species associated with those symptoms, contributing to updating the lettuce phytosanitary status in this region. The presence of orthotospovirus was confirmed by the enzyme-linked immunosorbent assay (DAS-ELISA), although the sequence of the nucleocapsid (N) gene confirmed the presence of Alstroemeria necrotic streak orthotospovirus, disregarding the Tomato spotted wilt orthotospovirus and Impatiens necrotic spot orthotospovirus previously reported for this crop, being this its first report in lettuce crops in Colombia.https://www.revistas.ufg.br/pat/article/view/68585/37033lactuca sativa l.tomato spotted wilt orthotospovirusimpatiens necrotic spot orthotospovirusnucleocapsid protein |
| spellingShingle | Diego Alejandro Jurado-Rincón Linda Jeimmy Rincón-Rivera Angela María Vargas-Berdugo Adriana González-Almario Partial sequencing of a putative Alstroemeria necrotic streak orthotospovirus isolate detected on lettuce in Colombia Pesquisa Agropecuária Tropical lactuca sativa l. tomato spotted wilt orthotospovirus impatiens necrotic spot orthotospovirus nucleocapsid protein |
| title | Partial sequencing of a putative Alstroemeria necrotic streak orthotospovirus isolate detected on lettuce in Colombia |
| title_full | Partial sequencing of a putative Alstroemeria necrotic streak orthotospovirus isolate detected on lettuce in Colombia |
| title_fullStr | Partial sequencing of a putative Alstroemeria necrotic streak orthotospovirus isolate detected on lettuce in Colombia |
| title_full_unstemmed | Partial sequencing of a putative Alstroemeria necrotic streak orthotospovirus isolate detected on lettuce in Colombia |
| title_short | Partial sequencing of a putative Alstroemeria necrotic streak orthotospovirus isolate detected on lettuce in Colombia |
| title_sort | partial sequencing of a putative alstroemeria necrotic streak orthotospovirus isolate detected on lettuce in colombia |
| topic | lactuca sativa l. tomato spotted wilt orthotospovirus impatiens necrotic spot orthotospovirus nucleocapsid protein |
| url | https://www.revistas.ufg.br/pat/article/view/68585/37033 |
| work_keys_str_mv | AT diegoalejandrojuradorincon partialsequencingofaputativealstroemerianecroticstreakorthotospovirusisolatedetectedonlettuceincolombia AT lindajeimmyrinconrivera partialsequencingofaputativealstroemerianecroticstreakorthotospovirusisolatedetectedonlettuceincolombia AT angelamariavargasberdugo partialsequencingofaputativealstroemerianecroticstreakorthotospovirusisolatedetectedonlettuceincolombia AT adrianagonzalezalmario partialsequencingofaputativealstroemerianecroticstreakorthotospovirusisolatedetectedonlettuceincolombia |