Production of Polycaprolactone / Gelatin / Polydimethylsiloxane Hybrid Nanofibers with Different Morphologies as Potential Scaffolds for Tissue Engineering
Background and Aim: Nowadays, research in the field of tissue engineering has increased widely. Scaffold production is important for regulation of the morphology of the tissues. The purpose of producing these scaffold is to make a uterine endometrial scaffold. Therefore, the fibers with different di...
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| Format: | Article |
| Language: | fas |
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Kurdistan University of Medical Sciences
2023-01-01
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| Series: | مجله علمی دانشگاه علوم پزشکی کردستان |
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| Online Access: | http://sjku.muk.ac.ir/article-1-6723-en.html |
| _version_ | 1797834615882252288 |
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| author | Mahdieh Dehghan Mohammad Khajeh Mehrizi Habib Nikukar |
| author_facet | Mahdieh Dehghan Mohammad Khajeh Mehrizi Habib Nikukar |
| author_sort | Mahdieh Dehghan |
| collection | DOAJ |
| description | Background and Aim: Nowadays, research in the field of tissue engineering has increased widely. Scaffold production is important for regulation of the morphology of the tissues. The purpose of producing these scaffold is to make a uterine endometrial scaffold. Therefore, the fibers with different diameters and morphology were synthesized to produce an optimal scaffold.
Materials and Methods: In this study, electrospun hybrid fiber scaffolds made of polycaprolactone (PCL), gelatin (G) and polydimethylsiloxane (PDMS) with different optimal ratios and different morphologies were produced by electrospinning collector changing for tissue engineering applications. Physicochemical properties of fabricated scaffolds were evaluated using scanning electron microscopy, the immersion of scaffold samples, attenuated total reflectance Fourier transform infrared and tensile strength analysis. Cytotoxicity analysis of scaffolds and human foreskin fibroblasts on the scaffolds were assessed by 3-(4, 5-dimethylthiazoyl-2-yl) 2, 5-diphenyltetrazolium bromide assay. Proliferation and growth of the cells on the scaffolds were evaluated by hematoxylin-eosin staining.
Results: Layer-by-layer scaffold, cotton-like scaffold, mixed cell scaffold, flat scaffold and control sample showed reduced cell growth and proliferation. Therefore, layer-by-layer scaffold and cotton-like scaffolds are the best options for cell growth and proliferation. Also, the mechanical properties of layer-by-layer scaffold and cotton-like scaffolds were better than those of other scaffolds. The cotton-like scaffold was better than other scaffolds in terms of porosity and was suitable for cell penetration into the scaffold. Although layer-by-layer and cell-mixed scaffolds showed suitable cell penetration due to placement of the cells among the fibers.
Conclusion: The morphology and characteristics of the PCL / G / PDMS hybrid scaffolds are adjustable by inducing change in the electrospinning collector. The PCL/ G/ PDMS hybrid scaffolds characteristics showed that these scaffolds were suitable for tissue engineering applications especially engineering of elastic tissues. |
| first_indexed | 2024-04-09T14:40:32Z |
| format | Article |
| id | doaj.art-bea8ade8c4d8474e867a9004a4e359ba |
| institution | Directory Open Access Journal |
| issn | 1560-652X 2345-4040 |
| language | fas |
| last_indexed | 2024-04-09T14:40:32Z |
| publishDate | 2023-01-01 |
| publisher | Kurdistan University of Medical Sciences |
| record_format | Article |
| series | مجله علمی دانشگاه علوم پزشکی کردستان |
| spelling | doaj.art-bea8ade8c4d8474e867a9004a4e359ba2023-05-03T08:14:16ZfasKurdistan University of Medical Sciencesمجله علمی دانشگاه علوم پزشکی کردستان1560-652X2345-40402023-01-012763753Production of Polycaprolactone / Gelatin / Polydimethylsiloxane Hybrid Nanofibers with Different Morphologies as Potential Scaffolds for Tissue EngineeringMahdieh Dehghan0Mohammad Khajeh Mehrizi1Habib Nikukar2 Textile Engineering Department, Faculty of Engineering, Yazd University, Yazd, Iran. Textile Engineering Department, Faculty of Engineering, Yazd University, Yazd, Iran Department of Advanced Medical Sciences and Technologies, School of Paramedicine, Shahid Sadoughi University of Medical Sciences, Yazd, Iran Background and Aim: Nowadays, research in the field of tissue engineering has increased widely. Scaffold production is important for regulation of the morphology of the tissues. The purpose of producing these scaffold is to make a uterine endometrial scaffold. Therefore, the fibers with different diameters and morphology were synthesized to produce an optimal scaffold. Materials and Methods: In this study, electrospun hybrid fiber scaffolds made of polycaprolactone (PCL), gelatin (G) and polydimethylsiloxane (PDMS) with different optimal ratios and different morphologies were produced by electrospinning collector changing for tissue engineering applications. Physicochemical properties of fabricated scaffolds were evaluated using scanning electron microscopy, the immersion of scaffold samples, attenuated total reflectance Fourier transform infrared and tensile strength analysis. Cytotoxicity analysis of scaffolds and human foreskin fibroblasts on the scaffolds were assessed by 3-(4, 5-dimethylthiazoyl-2-yl) 2, 5-diphenyltetrazolium bromide assay. Proliferation and growth of the cells on the scaffolds were evaluated by hematoxylin-eosin staining. Results: Layer-by-layer scaffold, cotton-like scaffold, mixed cell scaffold, flat scaffold and control sample showed reduced cell growth and proliferation. Therefore, layer-by-layer scaffold and cotton-like scaffolds are the best options for cell growth and proliferation. Also, the mechanical properties of layer-by-layer scaffold and cotton-like scaffolds were better than those of other scaffolds. The cotton-like scaffold was better than other scaffolds in terms of porosity and was suitable for cell penetration into the scaffold. Although layer-by-layer and cell-mixed scaffolds showed suitable cell penetration due to placement of the cells among the fibers. Conclusion: The morphology and characteristics of the PCL / G / PDMS hybrid scaffolds are adjustable by inducing change in the electrospinning collector. The PCL/ G/ PDMS hybrid scaffolds characteristics showed that these scaffolds were suitable for tissue engineering applications especially engineering of elastic tissues.http://sjku.muk.ac.ir/article-1-6723-en.htmlscaffold morphologynanofibersfibroblaststissue engineeringelectrospinning collector |
| spellingShingle | Mahdieh Dehghan Mohammad Khajeh Mehrizi Habib Nikukar Production of Polycaprolactone / Gelatin / Polydimethylsiloxane Hybrid Nanofibers with Different Morphologies as Potential Scaffolds for Tissue Engineering مجله علمی دانشگاه علوم پزشکی کردستان scaffold morphology nanofibers fibroblasts tissue engineering electrospinning collector |
| title | Production of Polycaprolactone / Gelatin / Polydimethylsiloxane Hybrid Nanofibers with Different Morphologies as Potential Scaffolds for Tissue Engineering |
| title_full | Production of Polycaprolactone / Gelatin / Polydimethylsiloxane Hybrid Nanofibers with Different Morphologies as Potential Scaffolds for Tissue Engineering |
| title_fullStr | Production of Polycaprolactone / Gelatin / Polydimethylsiloxane Hybrid Nanofibers with Different Morphologies as Potential Scaffolds for Tissue Engineering |
| title_full_unstemmed | Production of Polycaprolactone / Gelatin / Polydimethylsiloxane Hybrid Nanofibers with Different Morphologies as Potential Scaffolds for Tissue Engineering |
| title_short | Production of Polycaprolactone / Gelatin / Polydimethylsiloxane Hybrid Nanofibers with Different Morphologies as Potential Scaffolds for Tissue Engineering |
| title_sort | production of polycaprolactone gelatin polydimethylsiloxane hybrid nanofibers with different morphologies as potential scaffolds for tissue engineering |
| topic | scaffold morphology nanofibers fibroblasts tissue engineering electrospinning collector |
| url | http://sjku.muk.ac.ir/article-1-6723-en.html |
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