Antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol-A from <em>Ecklonia cava</em> against <em>Propionibacterium acnes</em>
Methanol extract of the marine brown alga Ecklonia cava has been shown to exhibit antimicrobial activity against Propionibacterium acnes. To identify the compounds conferring this antimicrobial activity, methanol extract was fractionated using silica-gel column chromatography by increasing solvent...
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| Format: | Article |
| Language: | English |
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Sociedad Botánica de México, A. C.
2014-08-01
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| Series: | Botanical Sciences |
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| Online Access: | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/102 |
| _version_ | 1828093875120504832 |
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| author | Jae-Suk Choi Kyungyong Lee Bo-Bae Lee Yang-Chun Kim Young Dae Kim Yong-Ki Hong Kwang Keun Cho In Soon Choi |
| author_facet | Jae-Suk Choi Kyungyong Lee Bo-Bae Lee Yang-Chun Kim Young Dae Kim Yong-Ki Hong Kwang Keun Cho In Soon Choi |
| author_sort | Jae-Suk Choi |
| collection | DOAJ |
| description |
Methanol extract of the marine brown alga Ecklonia cava has been shown to exhibit antimicrobial activity against Propionibacterium acnes. To identify the compounds conferring this antimicrobial activity, methanol extract was fractionated using silica-gel column chromatography by increasing solvent polarity, and the antimicrobial activities of each fraction were determined in terms of the minimum inhibitory concentration. Hexane, diethyl ether, acetone, ethyl acetate, acetonitrile, methanol, and water fractions were generated, with the acetone fraction exhibiting the greatest antibacterial activity (minimum inhibitory concentration = 156μg/mL). The acetone fraction was further purified by Sephadex LH-20 column chromatography and reverse-phase HPLC using an Alltima C18 column with an H2O-MeOH linear gradient from 30 to 100%. Two peaks were eluted at 18.1 (P1) and 23.8 (P2) min, both of which exhibited marked antibacterial activity (minimum inhibitory concentration = 39μg/mL for both). The structures of these compounds were determined on the basis of 1H and 13C nuclear magnetic resonance data and, through comparison with published data, can be phloroglucinol derivatives dieckol (P1) and phlorofucofuroeckol-A (P2). These results suggest that phlorotannins derived from E. cava have antimicrobial activity against P. acnes, and so may be useful as natural additives in antiacne cosmetics and pharmaceutical products.
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| first_indexed | 2024-04-11T06:50:33Z |
| format | Article |
| id | doaj.art-beaa36aedee14e68b733b0443064075c |
| institution | Directory Open Access Journal |
| issn | 2007-4298 2007-4476 |
| language | English |
| last_indexed | 2024-04-11T06:50:33Z |
| publishDate | 2014-08-01 |
| publisher | Sociedad Botánica de México, A. C. |
| record_format | Article |
| series | Botanical Sciences |
| spelling | doaj.art-beaa36aedee14e68b733b0443064075c2022-12-22T04:39:14ZengSociedad Botánica de México, A. C.Botanical Sciences2007-42982007-44762014-08-0192310.17129/botsci.102Antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol-A from <em>Ecklonia cava</em> against <em>Propionibacterium acnes</em>Jae-Suk Choi0Kyungyong Lee1Bo-Bae Lee2Yang-Chun Kim3Young Dae Kim4Yong-Ki Hong5Kwang Keun Cho6In Soon Choi7RIS Center, Industry-Academic Cooperation Foundation, Silla UniversityGijang Local Products Co. Ltd.RIS Center, Industry-Academic Cooperation Foundation, Silla University.Gijang Local Products Co. Ltd.East Sea Fisheries Research Institute, National Fisheries Research & Development Institute.Department of Biotechnology, Pukyong National University.Department of Animal Resources Technology, Gyeongnam National University of Science and Technology.RIS Center, Industry-Academic Cooperation Foundation, Depertment of Biological Science, Silla University. Methanol extract of the marine brown alga Ecklonia cava has been shown to exhibit antimicrobial activity against Propionibacterium acnes. To identify the compounds conferring this antimicrobial activity, methanol extract was fractionated using silica-gel column chromatography by increasing solvent polarity, and the antimicrobial activities of each fraction were determined in terms of the minimum inhibitory concentration. Hexane, diethyl ether, acetone, ethyl acetate, acetonitrile, methanol, and water fractions were generated, with the acetone fraction exhibiting the greatest antibacterial activity (minimum inhibitory concentration = 156μg/mL). The acetone fraction was further purified by Sephadex LH-20 column chromatography and reverse-phase HPLC using an Alltima C18 column with an H2O-MeOH linear gradient from 30 to 100%. Two peaks were eluted at 18.1 (P1) and 23.8 (P2) min, both of which exhibited marked antibacterial activity (minimum inhibitory concentration = 39μg/mL for both). The structures of these compounds were determined on the basis of 1H and 13C nuclear magnetic resonance data and, through comparison with published data, can be phloroglucinol derivatives dieckol (P1) and phlorofucofuroeckol-A (P2). These results suggest that phlorotannins derived from E. cava have antimicrobial activity against P. acnes, and so may be useful as natural additives in antiacne cosmetics and pharmaceutical products. https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/102dieckolminimum inhibitory concentrationnatural additivesphlorofucofuroeckol-Aphlorotannins |
| spellingShingle | Jae-Suk Choi Kyungyong Lee Bo-Bae Lee Yang-Chun Kim Young Dae Kim Yong-Ki Hong Kwang Keun Cho In Soon Choi Antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol-A from <em>Ecklonia cava</em> against <em>Propionibacterium acnes</em> Botanical Sciences dieckol minimum inhibitory concentration natural additives phlorofucofuroeckol-A phlorotannins |
| title | Antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol-A from <em>Ecklonia cava</em> against <em>Propionibacterium acnes</em> |
| title_full | Antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol-A from <em>Ecklonia cava</em> against <em>Propionibacterium acnes</em> |
| title_fullStr | Antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol-A from <em>Ecklonia cava</em> against <em>Propionibacterium acnes</em> |
| title_full_unstemmed | Antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol-A from <em>Ecklonia cava</em> against <em>Propionibacterium acnes</em> |
| title_short | Antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol-A from <em>Ecklonia cava</em> against <em>Propionibacterium acnes</em> |
| title_sort | antibacterial activity of the phlorotannins dieckol and phlorofucofuroeckol a from em ecklonia cava em against em propionibacterium acnes em |
| topic | dieckol minimum inhibitory concentration natural additives phlorofucofuroeckol-A phlorotannins |
| url | https://www.botanicalsciences.com.mx/index.php/botanicalSciences/article/view/102 |
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