Serum and Urine Biomarkers Related to Kidney Fibrosis Predict Kidney Outcome in Czech Patients with IgA Nephropathy
We evaluated biomarkers related to kidney fibrosis for the outcome of patients with IgA nephropathy (IgAN). Clinical parameters (estimated glomerular filtration rate, hypertension, proteinuria) and histological findings were assessed in 134 patients with IgAN at the time of diagnosis and followed up...
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2064 |
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| author | Michaela Neprasova Dita Maixnerova Nadja Sparding Federica Genovese Morten Asser Karsdal Helena Koprivova Marek Kollar Miloslav Suchanek Zdenka Hruskova Vladimir Tesar |
| author_facet | Michaela Neprasova Dita Maixnerova Nadja Sparding Federica Genovese Morten Asser Karsdal Helena Koprivova Marek Kollar Miloslav Suchanek Zdenka Hruskova Vladimir Tesar |
| author_sort | Michaela Neprasova |
| collection | DOAJ |
| description | We evaluated biomarkers related to kidney fibrosis for the outcome of patients with IgA nephropathy (IgAN). Clinical parameters (estimated glomerular filtration rate, hypertension, proteinuria) and histological findings were assessed in 134 patients with IgAN at the time of diagnosis and followed up prospectively (mean follow-up time, 56.5 months). We measured biomarkers of collagen and laminin turnover in serum and urine collected at the time of kidney biopsy using a novel enzyme-linked immunosorbent assay. Linear discriminant analysis and logistic regression models were used to predict the patient’s kidney outcome. Five serum and urine biomarkers of laminin and collagen turnover (sLG1M, sPRO-C3, sPRO-C6, uPRO-C6/Cr, uC3M/Cr) could significantly differentiae IgAN patients with a worse prognosis. Clinical parameters (glomerular filtration rate (GFR), proteinuria) distinguished patients at risk of IgAN progression with a specificity of 87.3% and a sensitivity of 45.2% (area under the curve-AUC 0.751). The addition of the biomarkers significantly increased the prognostic ability with a specificity of 85.1% and a sensitivity of 73.3% (AUC 0.905). We have identified three serum (sLG1M, sPRO-C3, sPRO-C6) and two urinary markers (uPRO-C6/Cr, u-C3M /Cr) that significantly improve the prognostic ability of markers of kidney function to identify an IgAN patient’s risk of progressing to ESKD. |
| first_indexed | 2024-03-11T09:43:08Z |
| format | Article |
| id | doaj.art-beb1bd4dd08e4bf4a501d4752905aca1 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:43:08Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-beb1bd4dd08e4bf4a501d4752905aca12023-11-16T16:51:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243206410.3390/ijms24032064Serum and Urine Biomarkers Related to Kidney Fibrosis Predict Kidney Outcome in Czech Patients with IgA NephropathyMichaela Neprasova0Dita Maixnerova1Nadja Sparding2Federica Genovese3Morten Asser Karsdal4Helena Koprivova5Marek Kollar6Miloslav Suchanek7Zdenka Hruskova8Vladimir Tesar9Department of Nephrology, 1st Faculty of Medicine and General University Hospital, Charles University, 128 08 Prague, Czech RepublicDepartment of Nephrology, 1st Faculty of Medicine and General University Hospital, Charles University, 128 08 Prague, Czech RepublicNordic Bioscience, 2730 Herlev, DenmarkNordic Bioscience, 2730 Herlev, DenmarkNordic Bioscience, 2730 Herlev, DenmarkInstitute of Medical Biochemistry and Laboratory Diagnostics, 1st Faculty of Medicine and General University Hospital, Charles University, 128 08 Prague, Czech RepublicDepartment of Clinical and Transplant Pathology, Institute of Clinical and Experimental Medicine, 140 21 Prague, Czech RepublicConsultant of Chemometrics, 140 00 Prague, Czech RepublicDepartment of Nephrology, 1st Faculty of Medicine and General University Hospital, Charles University, 128 08 Prague, Czech RepublicDepartment of Nephrology, 1st Faculty of Medicine and General University Hospital, Charles University, 128 08 Prague, Czech RepublicWe evaluated biomarkers related to kidney fibrosis for the outcome of patients with IgA nephropathy (IgAN). Clinical parameters (estimated glomerular filtration rate, hypertension, proteinuria) and histological findings were assessed in 134 patients with IgAN at the time of diagnosis and followed up prospectively (mean follow-up time, 56.5 months). We measured biomarkers of collagen and laminin turnover in serum and urine collected at the time of kidney biopsy using a novel enzyme-linked immunosorbent assay. Linear discriminant analysis and logistic regression models were used to predict the patient’s kidney outcome. Five serum and urine biomarkers of laminin and collagen turnover (sLG1M, sPRO-C3, sPRO-C6, uPRO-C6/Cr, uC3M/Cr) could significantly differentiae IgAN patients with a worse prognosis. Clinical parameters (glomerular filtration rate (GFR), proteinuria) distinguished patients at risk of IgAN progression with a specificity of 87.3% and a sensitivity of 45.2% (area under the curve-AUC 0.751). The addition of the biomarkers significantly increased the prognostic ability with a specificity of 85.1% and a sensitivity of 73.3% (AUC 0.905). We have identified three serum (sLG1M, sPRO-C3, sPRO-C6) and two urinary markers (uPRO-C6/Cr, u-C3M /Cr) that significantly improve the prognostic ability of markers of kidney function to identify an IgAN patient’s risk of progressing to ESKD.https://www.mdpi.com/1422-0067/24/3/2064IgA nephropathybiomarkersPRO-C6PRO-C3LG1MC3M |
| spellingShingle | Michaela Neprasova Dita Maixnerova Nadja Sparding Federica Genovese Morten Asser Karsdal Helena Koprivova Marek Kollar Miloslav Suchanek Zdenka Hruskova Vladimir Tesar Serum and Urine Biomarkers Related to Kidney Fibrosis Predict Kidney Outcome in Czech Patients with IgA Nephropathy International Journal of Molecular Sciences IgA nephropathy biomarkers PRO-C6 PRO-C3 LG1M C3M |
| title | Serum and Urine Biomarkers Related to Kidney Fibrosis Predict Kidney Outcome in Czech Patients with IgA Nephropathy |
| title_full | Serum and Urine Biomarkers Related to Kidney Fibrosis Predict Kidney Outcome in Czech Patients with IgA Nephropathy |
| title_fullStr | Serum and Urine Biomarkers Related to Kidney Fibrosis Predict Kidney Outcome in Czech Patients with IgA Nephropathy |
| title_full_unstemmed | Serum and Urine Biomarkers Related to Kidney Fibrosis Predict Kidney Outcome in Czech Patients with IgA Nephropathy |
| title_short | Serum and Urine Biomarkers Related to Kidney Fibrosis Predict Kidney Outcome in Czech Patients with IgA Nephropathy |
| title_sort | serum and urine biomarkers related to kidney fibrosis predict kidney outcome in czech patients with iga nephropathy |
| topic | IgA nephropathy biomarkers PRO-C6 PRO-C3 LG1M C3M |
| url | https://www.mdpi.com/1422-0067/24/3/2064 |
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