Evaluation of the effect of carbon nanoparticles on the proliferation of calli of date palm (Majol cultivar)
Objective Nanotechnology, as a promising method for addressing sustainable agricultural issues, can increase propagation efficiency in palm tissue culture. This study aimed to prepare carbon nanoparticles and use the resulting nanocomposites to evaluate their efficiency in improving and increasing d...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | fas |
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Shahid Bahonar University of Kerman
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_3511_4379a4f638c2766f3e4e4a5c5a11ef62.pdf |
| _version_ | 1797389035407147008 |
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| author | Sadaf Abedi Leila Ahangar Reza Zarghami Leila Maˈmani Masome Naeemi |
| author_facet | Sadaf Abedi Leila Ahangar Reza Zarghami Leila Maˈmani Masome Naeemi |
| author_sort | Sadaf Abedi |
| collection | DOAJ |
| description | Objective
Nanotechnology, as a promising method for addressing sustainable agricultural issues, can increase propagation efficiency in palm tissue culture. This study aimed to prepare carbon nanoparticles and use the resulting nanocomposites to evaluate their efficiency in improving and increasing date callus formation.
Materials and methods
Three separate experiments were performed to propagate calluses consisting of meristematic microsamples of dates. In the first experiment, calli prepared in MS culture medium were transferred to four culture media with different hormonal treatments from NAA and 2iP, and in the second experiment, calli composed of meristematic date microsamples in MS culture medium were transferred to four culture media with separate treatments from NAA and BAP. Different hormones were transferred from NAA and BAP. In the third experiment, after determining the best hormonal treatments from the first and second experiments, carbon nanoparticles were synthesized from graphite, and calli composed of meristematic date microsomal samples were recreated in superior culture media with different concentrations of nanoparticles (0, 10, 20, 30, 40, 50 mg/L).
Results
Based on the results of the first experiment, treatments of 10 mg/L NAA + 30 mg/L 2ip and 0.1 mg/L NAA + 0.05 mg/L 2ip were selected as the best callus propagation treatments. In the second experiment, it was found that there was no statistically significant difference between the applied treatments of 10 mg/L NAA + 30 mg/L BAP and 10 mg/L NAA + 10 mg/L BAP with other treatments with different concentrations of BAP. The results of the third experiment showed that the use of 10 mg/L NAA + 30 mg/L BAP + 30 mg/L CNP can produce the most calluses.
Conclusions
Due to the positive effect of carbon nanoparticles on increasing the weight of commodities, treatment of 10 mg/L NAA + 30 mg/L BAP + 30 mg/L CNP becomes the most suitable option for calorific cultivar propagation. |
| first_indexed | 2024-03-08T22:49:58Z |
| format | Article |
| id | doaj.art-beb8b148c4f047e38a754834dee0f526 |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-03-08T22:49:58Z |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-beb8b148c4f047e38a754834dee0f5262023-12-16T17:42:01ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-6500144214410.22103/jab.2022.19357.13973511Evaluation of the effect of carbon nanoparticles on the proliferation of calli of date palm (Majol cultivar)Sadaf Abedi0Leila Ahangar1Reza Zarghami2Leila Maˈmani3Masome Naeemi4MSC Student, Department of Plant Production, Faculty of Agricultural Sciences and Natural Resources, Gonbad Kavous UniversityAssistant Professor, Department of Plant Production, Faculty of Agricultural Sciences and Natural Resources, Gonbad Kavous UniversityFaculty member of Agricultural Biotechnology Research Institute (ABRII), Agricultural Research, Education and Extension Organization (AREEO), KarajFaculty member of Agricultural Biotechnology Research Institute (ABRII), Agricultural Research, Education and Extension Organization (AREEO), KarajAssistant Professor, Department of Plant Production, Faculty of Agricultural Sciences and Natural Resources, Gonbad Kavous UniversityObjective Nanotechnology, as a promising method for addressing sustainable agricultural issues, can increase propagation efficiency in palm tissue culture. This study aimed to prepare carbon nanoparticles and use the resulting nanocomposites to evaluate their efficiency in improving and increasing date callus formation. Materials and methods Three separate experiments were performed to propagate calluses consisting of meristematic microsamples of dates. In the first experiment, calli prepared in MS culture medium were transferred to four culture media with different hormonal treatments from NAA and 2iP, and in the second experiment, calli composed of meristematic date microsamples in MS culture medium were transferred to four culture media with separate treatments from NAA and BAP. Different hormones were transferred from NAA and BAP. In the third experiment, after determining the best hormonal treatments from the first and second experiments, carbon nanoparticles were synthesized from graphite, and calli composed of meristematic date microsomal samples were recreated in superior culture media with different concentrations of nanoparticles (0, 10, 20, 30, 40, 50 mg/L). Results Based on the results of the first experiment, treatments of 10 mg/L NAA + 30 mg/L 2ip and 0.1 mg/L NAA + 0.05 mg/L 2ip were selected as the best callus propagation treatments. In the second experiment, it was found that there was no statistically significant difference between the applied treatments of 10 mg/L NAA + 30 mg/L BAP and 10 mg/L NAA + 10 mg/L BAP with other treatments with different concentrations of BAP. The results of the third experiment showed that the use of 10 mg/L NAA + 30 mg/L BAP + 30 mg/L CNP can produce the most calluses. Conclusions Due to the positive effect of carbon nanoparticles on increasing the weight of commodities, treatment of 10 mg/L NAA + 30 mg/L BAP + 30 mg/L CNP becomes the most suitable option for calorific cultivar propagation.https://jab.uk.ac.ir/article_3511_4379a4f638c2766f3e4e4a5c5a11ef62.pdfdatesnanotechnologytissue culturecarbon nanoparticles |
| spellingShingle | Sadaf Abedi Leila Ahangar Reza Zarghami Leila Maˈmani Masome Naeemi Evaluation of the effect of carbon nanoparticles on the proliferation of calli of date palm (Majol cultivar) مجله بیوتکنولوژی کشاورزی dates nanotechnology tissue culture carbon nanoparticles |
| title | Evaluation of the effect of carbon nanoparticles on the proliferation of calli of date palm (Majol cultivar) |
| title_full | Evaluation of the effect of carbon nanoparticles on the proliferation of calli of date palm (Majol cultivar) |
| title_fullStr | Evaluation of the effect of carbon nanoparticles on the proliferation of calli of date palm (Majol cultivar) |
| title_full_unstemmed | Evaluation of the effect of carbon nanoparticles on the proliferation of calli of date palm (Majol cultivar) |
| title_short | Evaluation of the effect of carbon nanoparticles on the proliferation of calli of date palm (Majol cultivar) |
| title_sort | evaluation of the effect of carbon nanoparticles on the proliferation of calli of date palm majol cultivar |
| topic | dates nanotechnology tissue culture carbon nanoparticles |
| url | https://jab.uk.ac.ir/article_3511_4379a4f638c2766f3e4e4a5c5a11ef62.pdf |
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