Methylation Status Of MTHFR Promoter And Oligozoospermia Risk: An Epigenetic Study And In Silico Analysis
Objective: In this study, we evaluated the effects of promoter methylation of MTHFR on oligozoospermia risk, followed by an in silico analysis. Materials and Methods: In a case-control study, semen samples were collected from infertile and healthy control men. MTHFR promoter region was amplified...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Royan Institute (ACECR), Tehran
2021-01-01
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| Series: | Cell Journal |
| Subjects: | |
| Online Access: | https://celljournal.org/journal/article/fulltext/methylation-status-of-mthfr-promoter-and-idiopathic-male-infertility-risk-an-epigenetic-study-and-in-silico-analysis.pdf |
| Summary: | Objective: In this study, we evaluated the effects of promoter methylation of MTHFR on oligozoospermia risk, followed by an
in silico analysis.
Materials and Methods: In a case-control study, semen samples were collected from infertile and healthy control men.
MTHFR promoter region was amplified by methylation-specific polymerase chain reaction (PCR). Finally, the promoter
region of MTHFR was analyzed by bioinformatics software.
Results: Our data revealed significant associations of CpG island promoter methylation with oligozoospermia in a
case-control study. In silico analysis showed that promoter contains a strong nucleosome exclusion region, a bonafide
CGIs, six PROSITE motifs without a defined TATA box and 14 transcription factor (TF) binding sites, which are directly
involved in spermatogenesis
Conclusion: Based on our findings, methylation of the MTHFR gene promoter region may be a risk factor for
oligozoospermia. However, this is a preliminary report representing data for future comprehensive studies to make
a clinical conclusion on the potential biomarker role of methylation of this promoter in elevating susceptibility to
oligozoospermia. |
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| ISSN: | 2228-5806 2228-5814 |