Epitope Mapping of Anti-Mouse CCR3 Monoclonal Antibodies Using Flow Cytometry

Epitope Mapping of Anti-Mouse CCR3 Monoclonal Antibodies Using Flow Cytometry

The CC chemokine receptor 3 (CCR3) is a receptor for CC chemokines, including CCL5/RANTES, CCL7/MCP-3, and CCL11/eotaxin. CCR3 is expressed on the surface of eosinophils, basophils, a subset of Th2 lymphocytes, mast cells, and airway epithelial cells. CCR3 and its ligands are involved in airway hype...

Full description

Bibliographic Details
Main Authors:	Nami Tateyama, Teizo Asano, Hiroyuki Suzuki, Guanjie Li, Takeo Yoshikawa, Tomohiro Tanaka, Mika K. Kaneko, Yukinari Kato
Format:	Article
Language:	English
Published:	MDPI AG 2022-12-01
Series:	Antibodies
Subjects:	mouse CCR3 monoclonal antibody epitope mapping alanine scanning flow cytometry
Online Access:	https://www.mdpi.com/2073-4468/11/4/75

Similar Items

Determination of the Binding Epitope of an Anti-Mouse CCR9 Monoclonal Antibody (C<sub>9</sub>Mab-24) Using the 1× Alanine and 2× Alanine-Substitution Method
by: Hiyori Kobayashi, et al.
Published: (2023-01-01)

Identification of the Binding Epitope of an Anti-Mouse CCR6 Monoclonal Antibody (C<sub>6</sub>Mab-13) Using 1× Alanine Scanning
by: Tomohiro Tanaka, et al.
Published: (2023-04-01)

Elucidation of the critical epitope of an anti-EGFR monoclonal antibody EMab-134
by: Mika K. Kaneko, et al.
Published: (2018-07-01)

Determination of critical epitope of PcMab-47 against human podocalyxin
by: Shunsuke Itai, et al.
Published: (2018-07-01)

Development of a Novel Anti-EpCAM Monoclonal Antibody for Various Applications
by: Guanjie Li, et al.
Published: (2022-06-01)

Epitope mapping of monoclonal antibodies: a comprehensive comparison of different technologies
by: Xibei Dang, et al.
Published: (2023-12-01)

Development of a Novel Anti−CD44 Monoclonal Antibody for Multiple Applications against Esophageal Squamous Cell Carcinomas
by: Nohara Goto, et al.
Published: (2022-05-01)

A Cancer-Specific Monoclonal Antibody against HER2 Exerts Antitumor Activities in Human Breast Cancer Xenograft Models
by: Mika K. Kaneko, et al.
Published: (2024-02-01)

Development of a Novel Anti-CD44 Variant 4 Monoclonal Antibody C<sub>44</sub>Mab-108 for Immunohistochemistry
by: Hiroyuki Suzuki, et al.
Published: (2023-02-01)

Development of Highly Sensitive Anti-Mouse HER2 Monoclonal Antibodies for Flow Cytometry
by: Tsunenori Ouchida, et al.
Published: (2023-08-01)

Development of a Novel Anti-CD44 Variant 5 Monoclonal Antibody C<sub>44</sub>Mab-3 for Multiple Applications against Pancreatic Carcinomas
by: Yuma Kudo, et al.
Published: (2023-04-01)

Development of a Novel Anti-CD44 Variant 8 Monoclonal Antibody C<sub>44</sub>Mab-94 against Gastric Carcinomas
by: Hiroyuki Suzuki, et al.
Published: (2023-07-01)

Development of a Novel Anti-CD44 Variant 7/8 Monoclonal Antibody, C<sub>44</sub>Mab-34, for Multiple Applications against Oral Carcinomas
by: Hiroyuki Suzuki, et al.
Published: (2023-04-01)

Development of a Novel Anti-CD44 Variant 6 Monoclonal Antibody C<sub>44</sub>Mab-9 for Multiple Applications against Colorectal Carcinomas
by: Ryo Ejima, et al.
Published: (2023-02-01)

Characterization of novel Omp31 antigenic epitopes of Brucella melitensis by monoclonal antibodies
by: Jinfeng Li, et al.
Published: (2017-05-01)

A Novel Anti-CD44 Variant 3 Monoclonal Antibody C<sub>44</sub>Mab-6 Was Established for Multiple Applications
by: Hiroyuki Suzuki, et al.
Published: (2023-05-01)

Development of Monoclonal Antibodies for Detection of Conserved and Variable Epitopes of Large Protein of Rabies Virus
by: Wen Zhao, et al.
Published: (2021-01-01)

Generation and Characterization of Siglec-F-Specific Monoclonal Antibodies
by: Sima Shahmohammadi-Farid, et al.
Published: (2017-12-01)

RIEDL tag: A novel pentapeptide tagging system for transmembrane protein purification
by: Teizo Asano, et al.
Published: (2020-09-01)

Neutralization of Zika virus by germline-like human monoclonal antibodies targeting cryptic epitopes on envelope domain III
by: Yanling Wu, et al.
Published: (2017-01-01)

Generation of Monoclonal Antibodies against Variable Epitopes of the M Protein of Rabies Virus
by: Jie Liu, et al.
Published: (2019-04-01)

Systematic Assessment of Chemokine Signaling at Chemokine Receptors CCR4, CCR7 and CCR10
by: Herman D. Lim, et al.
Published: (2021-04-01)

Expression of ASFV p17 in CHO cells and identification of one novel epitope using a monoclonal antibody
by: Liwei Li, et al.
Published: (2023-10-01)

Unambiguous identification of α-Gal epitopes in intact monoclonal antibodies by NMR spectroscopy
by: Arthur Hinterholzer, et al.
Published: (2022-12-01)

Linear epitope identification of monoclonal antibodies against the duck Tembusu virus NS1
by: Hantai Tan, et al.
Published: (2023-10-01)

CCR5 Receptor Occupancy Analysis Reveals Increased Peripheral Blood CCR5+CD4+ T Cells Following Treatment With the Anti-CCR5 Antibody Leronlimab
by: Xiao L. Chang, et al.
Published: (2021-11-01)

A Defucosylated Anti-EpCAM Monoclonal Antibody (EpMab-37-mG<sub>2a</sub>-f) Exerts Antitumor Activity in Xenograft Model
by: Teizo Asano, et al.
Published: (2022-11-01)

Identification of Two Novel Linear Neutralizing Epitopes within the Hexon Protein of Canine Adenovirus Using Monoclonal Antibodies
by: Shujie Wang, et al.
Published: (2021-02-01)

Preparation of a novel monoclonal antibody against Avian leukosis virus subgroup J Gp85 protein and identification of its epitope
by: Houkun Wang, et al.
Published: (2021-07-01)

Identification of Linear B Cell Epitopes on CD2V Protein of African Swine Fever Virus by Monoclonal Antibodies
by: Rui Jia, et al.
Published: (2022-04-01)

CCR3, CCR4, CCR5, and CXCR3 expression in peripheral blood CD4+ lymphocytes in gastric cancer patients
by: Alireza Andalib, et al.
Published: (2013-01-01)

Preparation of Monoclonal Antibodies against the Capsid Protein and Development of an Epitope-Blocking Enzyme-Linked Immunosorbent Assay for Detection of the Antibody against Porcine Circovirus 3
by: Junli Wang, et al.
Published: (2024-01-01)

Targeting CCR5 as a Component of an HIV-1 Therapeutic Strategy
by: Hager Mohamed, et al.
Published: (2022-01-01)

Identification of a novel linear B-cell epitope using a monoclonal antibody against the carboxy terminus of the canine distemper virus nucleoprotein and sequence analysis of the identified epitope in different CDV isolates
by: Li Yi, et al.
Published: (2017-09-01)

Epitope mapping for the anti-rabbit cholesteryl ester transfer protein monoclonal antibody that selectively inhibits triglyceride transfer
by: Kazunori Saito, et al.
Published: (1999-11-01)

West Nile virus: characterization and diagnostic applications of monoclonal antibodies
by: Lelli Davide, et al.
Published: (2012-04-01)

Pharmacological Evidence of the Important Roles of CCR1 and CCR3 and Their Endogenous Ligands CCL2/7/8 in Hypersensitivity Based on a Murine Model of Neuropathic Pain
by: Katarzyna Pawlik, et al.
Published: (2022-12-01)

HLA-DQ-Specific Recombinant Human Monoclonal Antibodies Allow for In-Depth Analysis of HLA-DQ Epitopes
by: Suzanne Bezstarosti, et al.
Published: (2022-01-01)

Antagonist of chemokine receptors CCR1 and CCR3
by: Ammit Alaina, et al.
Published: (2000-11-01)

EPITOPES IDENTIFICATION OF BROADLY NEUTRALIZING MONOCLONAL ANTIBODIES AGAINST Corynebacterium diphtheriae EXOTOXIN
by: Ya.S. Kulyk, et al.
Published: (2022-08-01)