Mitogen-Activated Protein Kinase 4-Regulated Metabolic Networks
Mitogen-activated protein kinase 4 (MPK4) was first identified as a negative regulator of systemic acquired resistance. It is also an important kinase involved in many other biological processes in plants, including cytokinesis, reproduction, and photosynthesis. <i>Arabidopsis thaliana</i&g...
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MDPI AG
2022-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/2/880 |
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| author | Chuwei Lin Aneirin Alan Lott Wei Zhu Craig P. Dufresne Sixue Chen |
| author_facet | Chuwei Lin Aneirin Alan Lott Wei Zhu Craig P. Dufresne Sixue Chen |
| author_sort | Chuwei Lin |
| collection | DOAJ |
| description | Mitogen-activated protein kinase 4 (MPK4) was first identified as a negative regulator of systemic acquired resistance. It is also an important kinase involved in many other biological processes in plants, including cytokinesis, reproduction, and photosynthesis. <i>Arabidopsis thaliana</i> <i>mpk4</i> mutant is dwarf and sterile. Previous omics studies including genomics, transcriptomics, and proteomics have revealed new functions of MPK4 in different biological processes. However, due to challenges in metabolomics, no study has touched upon the metabolomic profiles of the <i>mpk4</i> mutant. What metabolites and metabolic pathways are potentially regulated by MPK4 are not known. Metabolites are crucial components of plants, and they play important roles in plant growth and development, signaling, and defense. Here we used targeted and untargeted metabolomics to profile metabolites in the wild type and the <i>mpk4</i> mutant. We found that in addition to the jasmonic acid and salicylic acid pathways, MPK4 is involved in polyamine synthesis and photosynthesis. In addition, we also conducted label-free proteomics of the two genotypes. The integration of metabolomics and proteomics data allows for an insight into the metabolomic networks that are potentially regulated by MPK4. |
| first_indexed | 2024-03-10T01:18:25Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T01:18:25Z |
| publishDate | 2022-01-01 |
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| spelling | doaj.art-bec5c118e6f24fe484da570973ab56632023-11-23T14:05:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-01-0123288010.3390/ijms23020880Mitogen-Activated Protein Kinase 4-Regulated Metabolic NetworksChuwei Lin0Aneirin Alan Lott1Wei Zhu2Craig P. Dufresne3Sixue Chen4Department of Biology, University of Florida, Gainesville, FL 32611, USADepartment of Biology, University of Florida, Gainesville, FL 32611, USADepartment of Biology, University of Florida, Gainesville, FL 32611, USAThermo Fisher Scientific, 1400 Northpoint Parkway, West Palm Beach, FL 33407, USADepartment of Biology, University of Florida, Gainesville, FL 32611, USAMitogen-activated protein kinase 4 (MPK4) was first identified as a negative regulator of systemic acquired resistance. It is also an important kinase involved in many other biological processes in plants, including cytokinesis, reproduction, and photosynthesis. <i>Arabidopsis thaliana</i> <i>mpk4</i> mutant is dwarf and sterile. Previous omics studies including genomics, transcriptomics, and proteomics have revealed new functions of MPK4 in different biological processes. However, due to challenges in metabolomics, no study has touched upon the metabolomic profiles of the <i>mpk4</i> mutant. What metabolites and metabolic pathways are potentially regulated by MPK4 are not known. Metabolites are crucial components of plants, and they play important roles in plant growth and development, signaling, and defense. Here we used targeted and untargeted metabolomics to profile metabolites in the wild type and the <i>mpk4</i> mutant. We found that in addition to the jasmonic acid and salicylic acid pathways, MPK4 is involved in polyamine synthesis and photosynthesis. In addition, we also conducted label-free proteomics of the two genotypes. The integration of metabolomics and proteomics data allows for an insight into the metabolomic networks that are potentially regulated by MPK4.https://www.mdpi.com/1422-0067/23/2/880<i>Arabidopsis thaliana</i>mitogen-activated protein kinase 4metabolomics and proteomicsimmunitycytokinesispolyamine metabolic networks |
| spellingShingle | Chuwei Lin Aneirin Alan Lott Wei Zhu Craig P. Dufresne Sixue Chen Mitogen-Activated Protein Kinase 4-Regulated Metabolic Networks International Journal of Molecular Sciences <i>Arabidopsis thaliana</i> mitogen-activated protein kinase 4 metabolomics and proteomics immunity cytokinesis polyamine metabolic networks |
| title | Mitogen-Activated Protein Kinase 4-Regulated Metabolic Networks |
| title_full | Mitogen-Activated Protein Kinase 4-Regulated Metabolic Networks |
| title_fullStr | Mitogen-Activated Protein Kinase 4-Regulated Metabolic Networks |
| title_full_unstemmed | Mitogen-Activated Protein Kinase 4-Regulated Metabolic Networks |
| title_short | Mitogen-Activated Protein Kinase 4-Regulated Metabolic Networks |
| title_sort | mitogen activated protein kinase 4 regulated metabolic networks |
| topic | <i>Arabidopsis thaliana</i> mitogen-activated protein kinase 4 metabolomics and proteomics immunity cytokinesis polyamine metabolic networks |
| url | https://www.mdpi.com/1422-0067/23/2/880 |
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