Observation of the protein expression level via naked eye: Pt clusters catalyze non-color molecules into brown-colored molecules in cells
αvβ3 is overexpressed in various tumor cells and plays a key role in tumor genesis, invasion, and metastasis. Therefore, it is of great significance to precisely detect the αvβ3 level in cells via a simple method. For this purpose, we have constructed a peptide-coated platinum (Pt) cluster. Due to i...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Chemistry |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fchem.2023.1145415/full |
| _version_ | 1811165289922953216 |
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| author | Dongfang Xia Yong Zhang Chunyu Zhang Xiuxiu Yao Yuhua Tang Fuchao Wang Xu Han Hongzong Yin Chao Xu Xueyun Gao |
| author_facet | Dongfang Xia Yong Zhang Chunyu Zhang Xiuxiu Yao Yuhua Tang Fuchao Wang Xu Han Hongzong Yin Chao Xu Xueyun Gao |
| author_sort | Dongfang Xia |
| collection | DOAJ |
| description | αvβ3 is overexpressed in various tumor cells and plays a key role in tumor genesis, invasion, and metastasis. Therefore, it is of great significance to precisely detect the αvβ3 level in cells via a simple method. For this purpose, we have constructed a peptide-coated platinum (Pt) cluster. Due to its bright fluorescence, well-defined Pt atom numbers, and peroxidase-like catalytic activity, this cluster can be used to evaluate αvβ3 levels in cells by fluorescence imaging, inductively coupled plasma mass spectrometry (ICP-MS), and catalytic amplification of visual dyes, respectively. In this report, the expression level of αvβ3 in living cells is well-detected by the naked eye under an ordinary light microscope when the Pt cluster binds to αvβ3 in cells and catalyzes non-color 3,3′-diaminobenzidine (DAB) into brown-colored molecules in situ. Moreover, SiHa, HeLa, and 16HBE cell lines with different αvβ3 expression levels can be visually distinguished by the peroxidase-like Pt clusters. This research will provide a reliable method for the simple detection of αvβ3 levels in cells. |
| first_indexed | 2024-04-10T15:34:51Z |
| format | Article |
| id | doaj.art-bec945bc0c414f0bad68b1c9a57e4c60 |
| institution | Directory Open Access Journal |
| issn | 2296-2646 |
| language | English |
| last_indexed | 2024-04-10T15:34:51Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Chemistry |
| spelling | doaj.art-bec945bc0c414f0bad68b1c9a57e4c602023-02-13T07:55:57ZengFrontiers Media S.A.Frontiers in Chemistry2296-26462023-02-011110.3389/fchem.2023.11454151145415Observation of the protein expression level via naked eye: Pt clusters catalyze non-color molecules into brown-colored molecules in cellsDongfang Xia0Yong Zhang1Chunyu Zhang2Xiuxiu Yao3Yuhua Tang4Fuchao Wang5Xu Han6Hongzong Yin7Chao Xu8Xueyun Gao9College of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, ChinaFaculty of Environment and Life, Beijing University of Technology, Beijing, ChinaInstitute of Materia Medica, Shandong First Medical University & Shandong Academy of Medical Sciences, Jinan, Shandong, ChinaFaculty of Environment and Life, Beijing University of Technology, Beijing, ChinaFaculty of Environment and Life, Beijing University of Technology, Beijing, ChinaCollege of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, ChinaCAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Multi-disciplinary Research Division, Institute of High Energy Physics and University of Chinese Academy of Sciences (UCAS), Chinese Academy of Sciences (CAS), Beijing, ChinaCollege of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, ChinaCollege of Chemistry and Material Science, Shandong Agricultural University, Taian, Shandong, ChinaFaculty of Environment and Life, Beijing University of Technology, Beijing, Chinaαvβ3 is overexpressed in various tumor cells and plays a key role in tumor genesis, invasion, and metastasis. Therefore, it is of great significance to precisely detect the αvβ3 level in cells via a simple method. For this purpose, we have constructed a peptide-coated platinum (Pt) cluster. Due to its bright fluorescence, well-defined Pt atom numbers, and peroxidase-like catalytic activity, this cluster can be used to evaluate αvβ3 levels in cells by fluorescence imaging, inductively coupled plasma mass spectrometry (ICP-MS), and catalytic amplification of visual dyes, respectively. In this report, the expression level of αvβ3 in living cells is well-detected by the naked eye under an ordinary light microscope when the Pt cluster binds to αvβ3 in cells and catalyzes non-color 3,3′-diaminobenzidine (DAB) into brown-colored molecules in situ. Moreover, SiHa, HeLa, and 16HBE cell lines with different αvβ3 expression levels can be visually distinguished by the peroxidase-like Pt clusters. This research will provide a reliable method for the simple detection of αvβ3 levels in cells.https://www.frontiersin.org/articles/10.3389/fchem.2023.1145415/fullPt clusterαvβ3peroxidase-like catalysistumor cellsdiaminobenzidine |
| spellingShingle | Dongfang Xia Yong Zhang Chunyu Zhang Xiuxiu Yao Yuhua Tang Fuchao Wang Xu Han Hongzong Yin Chao Xu Xueyun Gao Observation of the protein expression level via naked eye: Pt clusters catalyze non-color molecules into brown-colored molecules in cells Frontiers in Chemistry Pt cluster αvβ3 peroxidase-like catalysis tumor cells diaminobenzidine |
| title | Observation of the protein expression level via naked eye: Pt clusters catalyze non-color molecules into brown-colored molecules in cells |
| title_full | Observation of the protein expression level via naked eye: Pt clusters catalyze non-color molecules into brown-colored molecules in cells |
| title_fullStr | Observation of the protein expression level via naked eye: Pt clusters catalyze non-color molecules into brown-colored molecules in cells |
| title_full_unstemmed | Observation of the protein expression level via naked eye: Pt clusters catalyze non-color molecules into brown-colored molecules in cells |
| title_short | Observation of the protein expression level via naked eye: Pt clusters catalyze non-color molecules into brown-colored molecules in cells |
| title_sort | observation of the protein expression level via naked eye pt clusters catalyze non color molecules into brown colored molecules in cells |
| topic | Pt cluster αvβ3 peroxidase-like catalysis tumor cells diaminobenzidine |
| url | https://www.frontiersin.org/articles/10.3389/fchem.2023.1145415/full |
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