Identification of ripening-related genes in strawberry fruit by cDNA-AFLP
An RNA fingerprinting study of strawberry receptacle and achene tissue was performed to identify candidate genes involved in fruit ripening. Quantitative cDNA-AFLP was used to detect differential gene expression in green, white, pink and red stages of fruit ripening. Based on hierarchical average li...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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University of Debrecen
2005-10-01
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| Series: | International Journal of Horticultural Science |
| Subjects: | |
| Online Access: | https://ojs.lib.unideb.hu/IJHS/article/view/602 |
| _version_ | 1818211224490344448 |
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| author | A. Balogh T. Koncz V. Tisza E. Kiss L. Heszk |
| author_facet | A. Balogh T. Koncz V. Tisza E. Kiss L. Heszk |
| author_sort | A. Balogh |
| collection | DOAJ |
| description | An RNA fingerprinting study of strawberry receptacle and achene tissue was performed to identify candidate genes involved in fruit ripening. Quantitative cDNA-AFLP was used to detect differential gene expression in green, white, pink and red stages of fruit ripening. Based on hierarchical average linkage clustering the differentially expressed genes formed three major groups, genes expressed only in green receptacle, genes expressed mainly in white, pink and red receptacle, and in achene. 130 transcript-derived fragments (TDFs) were isolated and sequenced. Most TDFs did not show any homology to sequences with known functions, others were homologous to genes involved in oxidative stress response, signal transduction, regulation of development and cell-wall metabolism. Novel genes, so far not associated with strawberry ripening and ripening in general, were identified, such as genes encoding a bHLH protein, putative nitrilase-related protein, putative HD-zip protein. The differential pattern of gene expression draws the attention to the significance of ripening induced-or repressed promoters in strawberry fruit, whose isolation and characterization can be useful tool for functional genomics. For this purpose nine cDNA-AFLP fragments related either to ontogeny or senescing were completed with 5'UTR aiming at more precise annotation and future promoter isolation. Although tens of potentially important transcriptome changes were identified, the function of many ripening induced genes remain unknown. |
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| id | doaj.art-bed167e4d5a34e849c3779fbcd3f935b |
| institution | Directory Open Access Journal |
| issn | 1585-0404 2676-931X |
| language | English |
| last_indexed | 2024-12-12T05:29:06Z |
| publishDate | 2005-10-01 |
| publisher | University of Debrecen |
| record_format | Article |
| series | International Journal of Horticultural Science |
| spelling | doaj.art-bed167e4d5a34e849c3779fbcd3f935b2022-12-22T00:36:23ZengUniversity of DebrecenInternational Journal of Horticultural Science1585-04042676-931X2005-10-0111410.31421/IJHS/11/4/602Identification of ripening-related genes in strawberry fruit by cDNA-AFLPA. Balogh0T. Koncz1V. Tisza2E. Kiss3L. Heszk4Department of Genetics and Plant Breeding, Szent István University, Páter K. u. 1, H-2103 Gödöllő, HungaryDepartment of Genetics and Plant Breeding, Szent István University, Páter K. u. 1, H-2103 Gödöllő, HungaryDepartment of Genetics and Plant Breeding, Szent István University, Páter K. u. 1, H-2103 Gödöllő, HungaryDepartment of Genetics and Plant Breeding, Szent István University, Páter K. u. 1, H-2103 Gödöllő, Hungary e-mail kiss.erzsebet@mkk.szie.h; HAS-SIU Research Group for Molecular Plant Breeding, Páter K. u. 1, H-2103 , HungaryDepartment of Genetics and Plant Breeding, Szent István University, Páter K. u. 1, H-2103 Gödöllő, Hungary e-mail kiss.erzsebet@mkk.szie.h; HAS-SIU Research Group for Molecular Plant Breeding, Páter K. u. 1, H-2103 , HungaryAn RNA fingerprinting study of strawberry receptacle and achene tissue was performed to identify candidate genes involved in fruit ripening. Quantitative cDNA-AFLP was used to detect differential gene expression in green, white, pink and red stages of fruit ripening. Based on hierarchical average linkage clustering the differentially expressed genes formed three major groups, genes expressed only in green receptacle, genes expressed mainly in white, pink and red receptacle, and in achene. 130 transcript-derived fragments (TDFs) were isolated and sequenced. Most TDFs did not show any homology to sequences with known functions, others were homologous to genes involved in oxidative stress response, signal transduction, regulation of development and cell-wall metabolism. Novel genes, so far not associated with strawberry ripening and ripening in general, were identified, such as genes encoding a bHLH protein, putative nitrilase-related protein, putative HD-zip protein. The differential pattern of gene expression draws the attention to the significance of ripening induced-or repressed promoters in strawberry fruit, whose isolation and characterization can be useful tool for functional genomics. For this purpose nine cDNA-AFLP fragments related either to ontogeny or senescing were completed with 5'UTR aiming at more precise annotation and future promoter isolation. Although tens of potentially important transcriptome changes were identified, the function of many ripening induced genes remain unknown.https://ojs.lib.unideb.hu/IJHS/article/view/602cDNA-AFLP transcript profilingFragaria x ananassa Duch.fruit ripening |
| spellingShingle | A. Balogh T. Koncz V. Tisza E. Kiss L. Heszk Identification of ripening-related genes in strawberry fruit by cDNA-AFLP International Journal of Horticultural Science cDNA-AFLP transcript profiling Fragaria x ananassa Duch. fruit ripening |
| title | Identification of ripening-related genes in strawberry fruit by cDNA-AFLP |
| title_full | Identification of ripening-related genes in strawberry fruit by cDNA-AFLP |
| title_fullStr | Identification of ripening-related genes in strawberry fruit by cDNA-AFLP |
| title_full_unstemmed | Identification of ripening-related genes in strawberry fruit by cDNA-AFLP |
| title_short | Identification of ripening-related genes in strawberry fruit by cDNA-AFLP |
| title_sort | identification of ripening related genes in strawberry fruit by cdna aflp |
| topic | cDNA-AFLP transcript profiling Fragaria x ananassa Duch. fruit ripening |
| url | https://ojs.lib.unideb.hu/IJHS/article/view/602 |
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