Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome
Abstract N-Acetylneuraminic acid is the most abundant sialic acid (SA) in humans and is expressed as the terminal sugar on intestinal mucus glycans. Several pathogenic bacteria harvest and display host SA on their own surfaces to evade Siglec-mediated host immunity. While previous studies have ident...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-83875-w |
| _version_ | 1818998124957401088 |
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| author | Zhen Han Peter S. Thuy-Boun Wayne Pfeiffer Vincent F. Vartabedian Ali Torkamani John R. Teijaro Dennis W. Wolan |
| author_facet | Zhen Han Peter S. Thuy-Boun Wayne Pfeiffer Vincent F. Vartabedian Ali Torkamani John R. Teijaro Dennis W. Wolan |
| author_sort | Zhen Han |
| collection | DOAJ |
| description | Abstract N-Acetylneuraminic acid is the most abundant sialic acid (SA) in humans and is expressed as the terminal sugar on intestinal mucus glycans. Several pathogenic bacteria harvest and display host SA on their own surfaces to evade Siglec-mediated host immunity. While previous studies have identified bacterial enzymes associated with SA catabolism, no reported methods permit the selective labeling, tracking, and quantitation of SA-presenting microbes within complex multi-microbial systems. We combined metabolic labeling, click chemistry, 16S rRNA gene, and whole-genome sequencing to track and identify SA-presenting microbes from a cultured human fecal microbiome. We isolated a new strain of Escherichia coli that incorporates SA onto its own surface and encodes for the nanT, neuA, and neuS genes necessary for harvesting and presenting SA. Our method is applicable to the identification of SA-presenting bacteria from human, animal, and environmental microbiomes, as well as providing an entry point for the investigation of surface-expressed SA-associated structures. |
| first_indexed | 2024-12-20T21:56:33Z |
| format | Article |
| id | doaj.art-bed3f99c1737468aab9e58ba008d9d56 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T21:56:33Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-bed3f99c1737468aab9e58ba008d9d562022-12-21T19:25:26ZengNature PortfolioScientific Reports2045-23222021-02-0111111210.1038/s41598-021-83875-wIdentification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiomeZhen Han0Peter S. Thuy-Boun1Wayne Pfeiffer2Vincent F. Vartabedian3Ali Torkamani4John R. Teijaro5Dennis W. Wolan6Department of Molecular Medicine, The Scripps Research InstituteDepartment of Molecular Medicine, The Scripps Research InstituteSan Diego Supercomputer Center, University of California, San DiegoDepartment of Immunology and Microbiology, The Scripps Research InstituteScripps Research Translational Institute, The Scripps Research InstituteDepartment of Immunology and Microbiology, The Scripps Research InstituteDepartment of Molecular Medicine, The Scripps Research InstituteAbstract N-Acetylneuraminic acid is the most abundant sialic acid (SA) in humans and is expressed as the terminal sugar on intestinal mucus glycans. Several pathogenic bacteria harvest and display host SA on their own surfaces to evade Siglec-mediated host immunity. While previous studies have identified bacterial enzymes associated with SA catabolism, no reported methods permit the selective labeling, tracking, and quantitation of SA-presenting microbes within complex multi-microbial systems. We combined metabolic labeling, click chemistry, 16S rRNA gene, and whole-genome sequencing to track and identify SA-presenting microbes from a cultured human fecal microbiome. We isolated a new strain of Escherichia coli that incorporates SA onto its own surface and encodes for the nanT, neuA, and neuS genes necessary for harvesting and presenting SA. Our method is applicable to the identification of SA-presenting bacteria from human, animal, and environmental microbiomes, as well as providing an entry point for the investigation of surface-expressed SA-associated structures.https://doi.org/10.1038/s41598-021-83875-w |
| spellingShingle | Zhen Han Peter S. Thuy-Boun Wayne Pfeiffer Vincent F. Vartabedian Ali Torkamani John R. Teijaro Dennis W. Wolan Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome Scientific Reports |
| title | Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome |
| title_full | Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome |
| title_fullStr | Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome |
| title_full_unstemmed | Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome |
| title_short | Identification of an N-acetylneuraminic acid-presenting bacteria isolated from a human microbiome |
| title_sort | identification of an n acetylneuraminic acid presenting bacteria isolated from a human microbiome |
| url | https://doi.org/10.1038/s41598-021-83875-w |
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