Co-Existence of Oxazolidinone Resistance Genes <i>cfr</i>(D) and <i>optrA</i> on Two <i>Streptococcus parasuis</i> Isolates from Swine
This study was performed to investigate the presence and characteristics of the oxazolidinone resistance genes <i>optrA</i> and <i>cfr</i>(D) in <i>Streptococcus parasuis</i>. In total, 36 <i>Streptococcus</i> isolates (30 <i>Streptococcus suis&l...
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MDPI AG
2023-04-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/12/5/825 |
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| author | Ning Han Jie Li Peng Wan Yu Pan Tiantian Xu Wenguang Xiong Zhenling Zeng |
| author_facet | Ning Han Jie Li Peng Wan Yu Pan Tiantian Xu Wenguang Xiong Zhenling Zeng |
| author_sort | Ning Han |
| collection | DOAJ |
| description | This study was performed to investigate the presence and characteristics of the oxazolidinone resistance genes <i>optrA</i> and <i>cfr</i>(D) in <i>Streptococcus parasuis</i>. In total, 36 <i>Streptococcus</i> isolates (30 <i>Streptococcus suis</i> isolates, 6 <i>Streptococcus parasuis</i> isolates) were collected from pig farms in China in 2020–2021, using PCR to determine the presence of <i>optrA</i> and <i>cfr</i>. Then, 2 of the 36 Streptococcus isolates were further processed as follows. Whole-genome sequencing and de novo assembly were employed to analyze the genetic environment of the <i>optrA</i> and <i>cfr</i>(D) genes. Conjugation and inverse PCR were employed to verify the transferability of <i>optrA</i> and <i>cfr</i>(D). The <i>optrA</i> and <i>cfr</i>(D) genes were identified in two <i>S. parasuis</i> strains named SS17 and SS20, respectively. The <i>optrA</i> of the two isolates was located on chromosomes invariably associated with the <i>araC</i> gene and Tn<i>554</i>, which carry the resistance genes <i>erm</i>(A) and <i>ant</i>(9). The two plasmids that carry <i>cfr</i>(D), pSS17 (7550 bp) and pSS20-1 (7550 bp) have 100% nucleotide sequence identity. The <i>cfr</i>(D) was flanked by GMP synthase and IS1202. The findings of this study extend the current knowledge of the genetic background of <i>optrA</i> and <i>cfr</i>(D) and indicate that Tn<i>554</i> and IS1202 may play an important role in the transmission of <i>optrA</i> and <i>cfr</i>(D), respectively. |
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| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-11T04:00:55Z |
| publishDate | 2023-04-01 |
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| series | Antibiotics |
| spelling | doaj.art-bed405760b9442f8a4db1f424641a3da2023-11-18T00:11:48ZengMDPI AGAntibiotics2079-63822023-04-0112582510.3390/antibiotics12050825Co-Existence of Oxazolidinone Resistance Genes <i>cfr</i>(D) and <i>optrA</i> on Two <i>Streptococcus parasuis</i> Isolates from SwineNing Han0Jie Li1Peng Wan2Yu Pan3Tiantian Xu4Wenguang Xiong5Zhenling Zeng6Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaGuangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, ChinaThis study was performed to investigate the presence and characteristics of the oxazolidinone resistance genes <i>optrA</i> and <i>cfr</i>(D) in <i>Streptococcus parasuis</i>. In total, 36 <i>Streptococcus</i> isolates (30 <i>Streptococcus suis</i> isolates, 6 <i>Streptococcus parasuis</i> isolates) were collected from pig farms in China in 2020–2021, using PCR to determine the presence of <i>optrA</i> and <i>cfr</i>. Then, 2 of the 36 Streptococcus isolates were further processed as follows. Whole-genome sequencing and de novo assembly were employed to analyze the genetic environment of the <i>optrA</i> and <i>cfr</i>(D) genes. Conjugation and inverse PCR were employed to verify the transferability of <i>optrA</i> and <i>cfr</i>(D). The <i>optrA</i> and <i>cfr</i>(D) genes were identified in two <i>S. parasuis</i> strains named SS17 and SS20, respectively. The <i>optrA</i> of the two isolates was located on chromosomes invariably associated with the <i>araC</i> gene and Tn<i>554</i>, which carry the resistance genes <i>erm</i>(A) and <i>ant</i>(9). The two plasmids that carry <i>cfr</i>(D), pSS17 (7550 bp) and pSS20-1 (7550 bp) have 100% nucleotide sequence identity. The <i>cfr</i>(D) was flanked by GMP synthase and IS1202. The findings of this study extend the current knowledge of the genetic background of <i>optrA</i> and <i>cfr</i>(D) and indicate that Tn<i>554</i> and IS1202 may play an important role in the transmission of <i>optrA</i> and <i>cfr</i>(D), respectively.https://www.mdpi.com/2079-6382/12/5/825<i>cfr</i>(D)<i>optrA</i>oxazolidinonesresistance<i>Streptococcus parasuis</i> |
| spellingShingle | Ning Han Jie Li Peng Wan Yu Pan Tiantian Xu Wenguang Xiong Zhenling Zeng Co-Existence of Oxazolidinone Resistance Genes <i>cfr</i>(D) and <i>optrA</i> on Two <i>Streptococcus parasuis</i> Isolates from Swine Antibiotics <i>cfr</i>(D) <i>optrA</i> oxazolidinones resistance <i>Streptococcus parasuis</i> |
| title | Co-Existence of Oxazolidinone Resistance Genes <i>cfr</i>(D) and <i>optrA</i> on Two <i>Streptococcus parasuis</i> Isolates from Swine |
| title_full | Co-Existence of Oxazolidinone Resistance Genes <i>cfr</i>(D) and <i>optrA</i> on Two <i>Streptococcus parasuis</i> Isolates from Swine |
| title_fullStr | Co-Existence of Oxazolidinone Resistance Genes <i>cfr</i>(D) and <i>optrA</i> on Two <i>Streptococcus parasuis</i> Isolates from Swine |
| title_full_unstemmed | Co-Existence of Oxazolidinone Resistance Genes <i>cfr</i>(D) and <i>optrA</i> on Two <i>Streptococcus parasuis</i> Isolates from Swine |
| title_short | Co-Existence of Oxazolidinone Resistance Genes <i>cfr</i>(D) and <i>optrA</i> on Two <i>Streptococcus parasuis</i> Isolates from Swine |
| title_sort | co existence of oxazolidinone resistance genes i cfr i d and i optra i on two i streptococcus parasuis i isolates from swine |
| topic | <i>cfr</i>(D) <i>optrA</i> oxazolidinones resistance <i>Streptococcus parasuis</i> |
| url | https://www.mdpi.com/2079-6382/12/5/825 |
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