Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor
Glycolipids are a class of biodegradable biosurfactants that are non-toxic and based on renewables, making them a sustainable alternative to petrochemical surfactants. Enzymatic synthesis allows a tailor-made production of these versatile compounds using sugar and fatty acid building blocks with rat...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-05-01
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| Series: | Catalysts |
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| Online Access: | https://www.mdpi.com/2073-4344/12/5/551 |
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| author | Rebecca Hollenbach Delphine Muller André Delavault Christoph Syldatk |
| author_facet | Rebecca Hollenbach Delphine Muller André Delavault Christoph Syldatk |
| author_sort | Rebecca Hollenbach |
| collection | DOAJ |
| description | Glycolipids are a class of biodegradable biosurfactants that are non-toxic and based on renewables, making them a sustainable alternative to petrochemical surfactants. Enzymatic synthesis allows a tailor-made production of these versatile compounds using sugar and fatty acid building blocks with rationalized structures for targeted applications. Therefore, glycolipids can be comprehensively designed to outcompete conventional surfactants regarding their physicochemical properties. However, enzymatic glycolipid processes are struggling with both sugars and fatty acid solubilities in reaction media. Thus, continuous flow processes represent a powerful tool in designing efficient syntheses of sugar esters. In this study, a continuous enzymatic glycolipid production catalyzed by Novozyme 435<sup>®</sup> is presented as an unprecedented concept. A biphasic aqueous–organic system was investigated, allowing for the simultaneous solubilization of sugars and fatty acids. Owing to phase separation, the remaining non-acylated glucose was easily separated from the product stream and was refed to the reactor forming a closed-loop system. Productivity in the continuous process was higher compared to a batch one, with space–time yields of up to 1228 ± 65 µmol/L/h. A temperature of 70 °C resulted in the highest glucose-6-<i>O</i>-decanoate concentration in the Packed Bed Reactor (PBR). Consequently, the design of a continuous biocatalytic production is a step towards a more competitive glycolipid synthesis in the aim for industrialization. |
| first_indexed | 2024-03-10T03:10:56Z |
| format | Article |
| id | doaj.art-bed5b48726914f23b28f0eda415a2d8d |
| institution | Directory Open Access Journal |
| issn | 2073-4344 |
| language | English |
| last_indexed | 2024-03-10T03:10:56Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Catalysts |
| spelling | doaj.art-bed5b48726914f23b28f0eda415a2d8d2023-11-23T10:26:31ZengMDPI AGCatalysts2073-43442022-05-0112555110.3390/catal12050551Continuous Flow Glycolipid Synthesis Using a Packed Bed ReactorRebecca Hollenbach0Delphine Muller1André Delavault2Christoph Syldatk3Technical Biology, Institute of Process Engineering in Life Sciences II, Karlsruhe Institute of Technology, 76131 Karlsruhe, GermanyTechnical Biology, Institute of Process Engineering in Life Sciences II, Karlsruhe Institute of Technology, 76131 Karlsruhe, GermanyTechnical Biology, Institute of Process Engineering in Life Sciences II, Karlsruhe Institute of Technology, 76131 Karlsruhe, GermanyTechnical Biology, Institute of Process Engineering in Life Sciences II, Karlsruhe Institute of Technology, 76131 Karlsruhe, GermanyGlycolipids are a class of biodegradable biosurfactants that are non-toxic and based on renewables, making them a sustainable alternative to petrochemical surfactants. Enzymatic synthesis allows a tailor-made production of these versatile compounds using sugar and fatty acid building blocks with rationalized structures for targeted applications. Therefore, glycolipids can be comprehensively designed to outcompete conventional surfactants regarding their physicochemical properties. However, enzymatic glycolipid processes are struggling with both sugars and fatty acid solubilities in reaction media. Thus, continuous flow processes represent a powerful tool in designing efficient syntheses of sugar esters. In this study, a continuous enzymatic glycolipid production catalyzed by Novozyme 435<sup>®</sup> is presented as an unprecedented concept. A biphasic aqueous–organic system was investigated, allowing for the simultaneous solubilization of sugars and fatty acids. Owing to phase separation, the remaining non-acylated glucose was easily separated from the product stream and was refed to the reactor forming a closed-loop system. Productivity in the continuous process was higher compared to a batch one, with space–time yields of up to 1228 ± 65 µmol/L/h. A temperature of 70 °C resulted in the highest glucose-6-<i>O</i>-decanoate concentration in the Packed Bed Reactor (PBR). Consequently, the design of a continuous biocatalytic production is a step towards a more competitive glycolipid synthesis in the aim for industrialization.https://www.mdpi.com/2073-4344/12/5/551continuous flowheterogenous biocatalysispacked bed reactorglycolipidbiphasic system<i>Candida antarctica</i> Lipase B |
| spellingShingle | Rebecca Hollenbach Delphine Muller André Delavault Christoph Syldatk Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor Catalysts continuous flow heterogenous biocatalysis packed bed reactor glycolipid biphasic system <i>Candida antarctica</i> Lipase B |
| title | Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor |
| title_full | Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor |
| title_fullStr | Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor |
| title_full_unstemmed | Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor |
| title_short | Continuous Flow Glycolipid Synthesis Using a Packed Bed Reactor |
| title_sort | continuous flow glycolipid synthesis using a packed bed reactor |
| topic | continuous flow heterogenous biocatalysis packed bed reactor glycolipid biphasic system <i>Candida antarctica</i> Lipase B |
| url | https://www.mdpi.com/2073-4344/12/5/551 |
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