Reflections of COVID-19 cases in the wastewater loading of SARS-CoV-2 RNA: A case of three major cities of Gujarat, India
Wastewater-based epidemiology (WBE) is a promising approach to understand the actual prevalence of COVID-19 disease at the community level. Different studies have cited the presence of SARS-CoV-2 in wastewater samples. In the present study, eighteen influent wastewater samples from different sewage...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-12-01
|
| Series: | Case Studies in Chemical and Environmental Engineering |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666016421000372 |
| _version_ | 1819180445405806592 |
|---|---|
| author | Vaibhav Srivastava Shilangi Gupta Arbind Kumar Patel Madhvi Joshi Manish Kumar |
| author_facet | Vaibhav Srivastava Shilangi Gupta Arbind Kumar Patel Madhvi Joshi Manish Kumar |
| author_sort | Vaibhav Srivastava |
| collection | DOAJ |
| description | Wastewater-based epidemiology (WBE) is a promising approach to understand the actual prevalence of COVID-19 disease at the community level. Different studies have cited the presence of SARS-CoV-2 in wastewater samples. In the present study, eighteen influent wastewater samples from different sewage treatment plants and pumping stations (5 samples from Vadodara city, 4 from Gandhinagar, and 9 from Ahmedabad city) were collected and analyzed for the presence of SARS-CoV-2 RNA in Gujarat state, India. The results showed the highest SARS-CoV-2 effective gene concentration in Vadodara (3078 copies/L), followed by Ahmedabad (2968 copies/L) and Gandhinagar (354 copies/L). On comparing the virus gene concentration in wastewater samples, the SARS-CoV-2 genetic material exhibited a positive relationship with the number of confirmed and active cases in in all three cities. However, a minor variation in SARS-CoV-2 effective gene concentration was seen between Vadodara and Ahmedabad despite a >2.5 and >1.5 folds differences in the cumulative number of confirmed and active cases, respectively. This may occur primarily due to the greater test positivity ratio in Vadodara (3.30%) than Ahmedabad (1.40%) and might be the higher number of asymptomatic patients in Vadodara. The study confirms the potential of the WBE that can be used at a large scale around the globe for better dealing with the pandemic situation. |
| first_indexed | 2024-12-22T22:14:27Z |
| format | Article |
| id | doaj.art-bed758412b1940ea8a83ab54fc38c52f |
| institution | Directory Open Access Journal |
| issn | 2666-0164 |
| language | English |
| last_indexed | 2024-12-22T22:14:27Z |
| publishDate | 2021-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Case Studies in Chemical and Environmental Engineering |
| spelling | doaj.art-bed758412b1940ea8a83ab54fc38c52f2022-12-21T18:10:48ZengElsevierCase Studies in Chemical and Environmental Engineering2666-01642021-12-014100115Reflections of COVID-19 cases in the wastewater loading of SARS-CoV-2 RNA: A case of three major cities of Gujarat, IndiaVaibhav Srivastava0Shilangi Gupta1Arbind Kumar Patel2Madhvi Joshi3Manish Kumar4Discipline of Earth Science, Indian Institute of Technology Gandhinagar, Gujarat, 382 355, IndiaDiscipline of Earth Science, Indian Institute of Technology Gandhinagar, Gujarat, 382 355, IndiaDiscipline of Earth Science, Indian Institute of Technology Gandhinagar, Gujarat, 382 355, IndiaGujarat Biotechnology Research Centre (GBRC), Sector- 11, Gandhinagar, Gujarat, 382 011, IndiaCorresponding author.,; Discipline of Earth Science, Indian Institute of Technology Gandhinagar, Gujarat, 382 355, India; Kiran C Patel Centre for Sustainable Development, Indian Institute of Technology Gandhinagar, Gujarat, IndiaWastewater-based epidemiology (WBE) is a promising approach to understand the actual prevalence of COVID-19 disease at the community level. Different studies have cited the presence of SARS-CoV-2 in wastewater samples. In the present study, eighteen influent wastewater samples from different sewage treatment plants and pumping stations (5 samples from Vadodara city, 4 from Gandhinagar, and 9 from Ahmedabad city) were collected and analyzed for the presence of SARS-CoV-2 RNA in Gujarat state, India. The results showed the highest SARS-CoV-2 effective gene concentration in Vadodara (3078 copies/L), followed by Ahmedabad (2968 copies/L) and Gandhinagar (354 copies/L). On comparing the virus gene concentration in wastewater samples, the SARS-CoV-2 genetic material exhibited a positive relationship with the number of confirmed and active cases in in all three cities. However, a minor variation in SARS-CoV-2 effective gene concentration was seen between Vadodara and Ahmedabad despite a >2.5 and >1.5 folds differences in the cumulative number of confirmed and active cases, respectively. This may occur primarily due to the greater test positivity ratio in Vadodara (3.30%) than Ahmedabad (1.40%) and might be the higher number of asymptomatic patients in Vadodara. The study confirms the potential of the WBE that can be used at a large scale around the globe for better dealing with the pandemic situation.http://www.sciencedirect.com/science/article/pii/S2666016421000372COVID-19SARS-CoV-2Wastewater based epidemiology (WBE)PandemicManagement |
| spellingShingle | Vaibhav Srivastava Shilangi Gupta Arbind Kumar Patel Madhvi Joshi Manish Kumar Reflections of COVID-19 cases in the wastewater loading of SARS-CoV-2 RNA: A case of three major cities of Gujarat, India Case Studies in Chemical and Environmental Engineering COVID-19 SARS-CoV-2 Wastewater based epidemiology (WBE) Pandemic Management |
| title | Reflections of COVID-19 cases in the wastewater loading of SARS-CoV-2 RNA: A case of three major cities of Gujarat, India |
| title_full | Reflections of COVID-19 cases in the wastewater loading of SARS-CoV-2 RNA: A case of three major cities of Gujarat, India |
| title_fullStr | Reflections of COVID-19 cases in the wastewater loading of SARS-CoV-2 RNA: A case of three major cities of Gujarat, India |
| title_full_unstemmed | Reflections of COVID-19 cases in the wastewater loading of SARS-CoV-2 RNA: A case of three major cities of Gujarat, India |
| title_short | Reflections of COVID-19 cases in the wastewater loading of SARS-CoV-2 RNA: A case of three major cities of Gujarat, India |
| title_sort | reflections of covid 19 cases in the wastewater loading of sars cov 2 rna a case of three major cities of gujarat india |
| topic | COVID-19 SARS-CoV-2 Wastewater based epidemiology (WBE) Pandemic Management |
| url | http://www.sciencedirect.com/science/article/pii/S2666016421000372 |
| work_keys_str_mv | AT vaibhavsrivastava reflectionsofcovid19casesinthewastewaterloadingofsarscov2rnaacaseofthreemajorcitiesofgujaratindia AT shilangigupta reflectionsofcovid19casesinthewastewaterloadingofsarscov2rnaacaseofthreemajorcitiesofgujaratindia AT arbindkumarpatel reflectionsofcovid19casesinthewastewaterloadingofsarscov2rnaacaseofthreemajorcitiesofgujaratindia AT madhvijoshi reflectionsofcovid19casesinthewastewaterloadingofsarscov2rnaacaseofthreemajorcitiesofgujaratindia AT manishkumar reflectionsofcovid19casesinthewastewaterloadingofsarscov2rnaacaseofthreemajorcitiesofgujaratindia |