Antibiotic Effect of High-Power Blue Laser Radiation
The development of sustainable alternatives to chemical and mechanical biofilm removal for submerged technical devices used in freshwater and marine environments represents a major technical challenge. In this context, the antibiotic impact of blue light with its low absorption underwater provides a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-02-01
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| Series: | Photonics |
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| Online Access: | https://www.mdpi.com/2304-6732/11/3/220 |
| _version_ | 1797239681208811520 |
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| author | Mattes Hintmann Stanislav Zimbelmann Benjamin Emde Rebekka Biedendieck Dieter Jahn |
| author_facet | Mattes Hintmann Stanislav Zimbelmann Benjamin Emde Rebekka Biedendieck Dieter Jahn |
| author_sort | Mattes Hintmann |
| collection | DOAJ |
| description | The development of sustainable alternatives to chemical and mechanical biofilm removal for submerged technical devices used in freshwater and marine environments represents a major technical challenge. In this context, the antibiotic impact of blue light with its low absorption underwater provides a potentially useful alternative. However, former technical limitations led to hours of treatment. Here, we applied high-power blue laser irradiation (1500 W) with a wavelength of 448 nm to demonstrate its strong antibiotic and algicidal effect on different bacteria and algae in seconds. High-power blue light treatment (139 W/cm<sup>2</sup>) for only 8.9 s led to the efficient deactivation of all tested organisms. Analyses of the underlying biological mechanisms revealed the absorption of the blue light by endogenous chromophores (flavins, tetrapyrroles) with the generation of reactive oxygen species (ROS). In agreement, <i>Escherichia coli</i> transcriptome analyses demonstrated a stress response at the level of DNA damage repair, respiration, and protein biosynthesis. Spectroscopic measurements of the irradiated algae indicated the irreversible damage of chlorophyll by photooxidation with the formation of singlet oxygen. In conclusion, high-power blue laser radiation provides a strong sustainable tool for the removal of biofouling in a very short time for applications in aquatic systems. |
| first_indexed | 2024-04-24T17:55:24Z |
| format | Article |
| id | doaj.art-beda007042df4327a591b39420c3fd55 |
| institution | Directory Open Access Journal |
| issn | 2304-6732 |
| language | English |
| last_indexed | 2024-04-24T17:55:24Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Photonics |
| spelling | doaj.art-beda007042df4327a591b39420c3fd552024-03-27T14:00:05ZengMDPI AGPhotonics2304-67322024-02-0111322010.3390/photonics11030220Antibiotic Effect of High-Power Blue Laser RadiationMattes Hintmann0Stanislav Zimbelmann1Benjamin Emde2Rebekka Biedendieck3Dieter Jahn4Institute of Microbiology and Braunschweig Integrated Centre of Systems Biology (BRICS), Technische Universität Braunschweig, Rebenring 56, 38106 Braunschweig, GermanyLaser Zentrum Hannover e. V., Hollerithallee 8, 30419 Hannover, GermanyLaser Zentrum Hannover e. V., Hollerithallee 8, 30419 Hannover, GermanyInstitute of Microbiology and Braunschweig Integrated Centre of Systems Biology (BRICS), Technische Universität Braunschweig, Rebenring 56, 38106 Braunschweig, GermanyInstitute of Microbiology and Braunschweig Integrated Centre of Systems Biology (BRICS), Technische Universität Braunschweig, Rebenring 56, 38106 Braunschweig, GermanyThe development of sustainable alternatives to chemical and mechanical biofilm removal for submerged technical devices used in freshwater and marine environments represents a major technical challenge. In this context, the antibiotic impact of blue light with its low absorption underwater provides a potentially useful alternative. However, former technical limitations led to hours of treatment. Here, we applied high-power blue laser irradiation (1500 W) with a wavelength of 448 nm to demonstrate its strong antibiotic and algicidal effect on different bacteria and algae in seconds. High-power blue light treatment (139 W/cm<sup>2</sup>) for only 8.9 s led to the efficient deactivation of all tested organisms. Analyses of the underlying biological mechanisms revealed the absorption of the blue light by endogenous chromophores (flavins, tetrapyrroles) with the generation of reactive oxygen species (ROS). In agreement, <i>Escherichia coli</i> transcriptome analyses demonstrated a stress response at the level of DNA damage repair, respiration, and protein biosynthesis. Spectroscopic measurements of the irradiated algae indicated the irreversible damage of chlorophyll by photooxidation with the formation of singlet oxygen. In conclusion, high-power blue laser radiation provides a strong sustainable tool for the removal of biofouling in a very short time for applications in aquatic systems.https://www.mdpi.com/2304-6732/11/3/220high-power blue light laseralgicidal effectbiofoulingchlorophyllphotoinactivationreactive oxygen species (ROS) |
| spellingShingle | Mattes Hintmann Stanislav Zimbelmann Benjamin Emde Rebekka Biedendieck Dieter Jahn Antibiotic Effect of High-Power Blue Laser Radiation Photonics high-power blue light laser algicidal effect biofouling chlorophyll photoinactivation reactive oxygen species (ROS) |
| title | Antibiotic Effect of High-Power Blue Laser Radiation |
| title_full | Antibiotic Effect of High-Power Blue Laser Radiation |
| title_fullStr | Antibiotic Effect of High-Power Blue Laser Radiation |
| title_full_unstemmed | Antibiotic Effect of High-Power Blue Laser Radiation |
| title_short | Antibiotic Effect of High-Power Blue Laser Radiation |
| title_sort | antibiotic effect of high power blue laser radiation |
| topic | high-power blue light laser algicidal effect biofouling chlorophyll photoinactivation reactive oxygen species (ROS) |
| url | https://www.mdpi.com/2304-6732/11/3/220 |
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