The Effect of Pre-Storage Irradiation Blood on Quality of Red Blood Cells
Background: Irradiation leads to increased storage lesions that may have harmful effects if transfused. Various storage lesions research has been carried out, and only very few articles are available on the impact of gamma irradiation on RBC storage lesions. Since there has been no study about findi...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2021-01-01
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| Series: | International Journal of Hematology-Oncology and Stem Cell Research |
| Subjects: | |
| Online Access: | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1254 |
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| author | Nahal Eshghifar Mahtab Maghsudlu Sedigheh Amini kafiabad |
| author_facet | Nahal Eshghifar Mahtab Maghsudlu Sedigheh Amini kafiabad |
| author_sort | Nahal Eshghifar |
| collection | DOAJ |
| description | Background: Irradiation leads to increased storage lesions that may have harmful effects if transfused. Various storage lesions research has been carried out, and only very few articles are available on the impact of gamma irradiation on RBC storage lesions. Since there has been no study about finding the best time for irradiation, we decided to investigate the effect of irradiation on Red blood cells at different storage times after blood collection
Materials and Methods: A total of 40 units of red blood cells divided into two groups: irradiated and non-irradiated. Irradiated RBCs were divided into three groups, each containing ten units. The remaining ten units were considered as non-irradiated controls. Sampling from these irradiated and non-irradiated blood units was performed weekly to evaluate biochemical parameters and free plasma hemoglobin/Hemolysis index levels.
Results: A significant increase in the mean values of plasma potassium, plasma Hb/Hemolysis index, and LDH, as well as a significant reduction in the mean value of 2,3 DPG and plasma sodium, were observed in both groups. Although the reduction of 2,3 DPG is extremely remarkable, it is compensated 24-48 hours after transfusion. Hence, the clinical result of 2,3-DPG-depleted RBC transfusion is known to be negligible. The irradiation group alteration was more notable than the non-irradiated one and the changes in the parameters were most significant in the group that was stored for a longer period after irradiation.
Conclusion: The investigation on the impact of gamma irradiation on RBCs makes it possible to suggest a storage time up to 28 days after irradiation and the best time for irradiation after blood collection is up to 14 days. It is pointed out that the blood unit should be transfused as soon as possible after the irradiation |
| first_indexed | 2024-03-12T10:13:05Z |
| format | Article |
| id | doaj.art-bedc957edce24ab9a2eec41185bb6033 |
| institution | Directory Open Access Journal |
| issn | 2008-2207 |
| language | English |
| last_indexed | 2024-03-12T10:13:05Z |
| publishDate | 2021-01-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | International Journal of Hematology-Oncology and Stem Cell Research |
| spelling | doaj.art-bedc957edce24ab9a2eec41185bb60332023-09-02T10:46:04ZengTehran University of Medical SciencesInternational Journal of Hematology-Oncology and Stem Cell Research2008-22072021-01-0115110.18502/ijhoscr.v15i1.5244The Effect of Pre-Storage Irradiation Blood on Quality of Red Blood CellsNahal Eshghifar0Mahtab Maghsudlu1Sedigheh Amini kafiabad2Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, IranBlood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, IranDepartment of Pathology, Iranian Blood Transfusion Organization, Research Centre, High Institute for Research and Education in Transfusion Medicine, Tehran, IranBackground: Irradiation leads to increased storage lesions that may have harmful effects if transfused. Various storage lesions research has been carried out, and only very few articles are available on the impact of gamma irradiation on RBC storage lesions. Since there has been no study about finding the best time for irradiation, we decided to investigate the effect of irradiation on Red blood cells at different storage times after blood collection Materials and Methods: A total of 40 units of red blood cells divided into two groups: irradiated and non-irradiated. Irradiated RBCs were divided into three groups, each containing ten units. The remaining ten units were considered as non-irradiated controls. Sampling from these irradiated and non-irradiated blood units was performed weekly to evaluate biochemical parameters and free plasma hemoglobin/Hemolysis index levels. Results: A significant increase in the mean values of plasma potassium, plasma Hb/Hemolysis index, and LDH, as well as a significant reduction in the mean value of 2,3 DPG and plasma sodium, were observed in both groups. Although the reduction of 2,3 DPG is extremely remarkable, it is compensated 24-48 hours after transfusion. Hence, the clinical result of 2,3-DPG-depleted RBC transfusion is known to be negligible. The irradiation group alteration was more notable than the non-irradiated one and the changes in the parameters were most significant in the group that was stored for a longer period after irradiation. Conclusion: The investigation on the impact of gamma irradiation on RBCs makes it possible to suggest a storage time up to 28 days after irradiation and the best time for irradiation after blood collection is up to 14 days. It is pointed out that the blood unit should be transfused as soon as possible after the irradiationhttps://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1254Red blood cells, Gamma irradiation, Storage lesions, Transfusion-associated graft versus host disease (TA-GVHD) |
| spellingShingle | Nahal Eshghifar Mahtab Maghsudlu Sedigheh Amini kafiabad The Effect of Pre-Storage Irradiation Blood on Quality of Red Blood Cells International Journal of Hematology-Oncology and Stem Cell Research Red blood cells, Gamma irradiation, Storage lesions, Transfusion-associated graft versus host disease (TA-GVHD) |
| title | The Effect of Pre-Storage Irradiation Blood on Quality of Red Blood Cells |
| title_full | The Effect of Pre-Storage Irradiation Blood on Quality of Red Blood Cells |
| title_fullStr | The Effect of Pre-Storage Irradiation Blood on Quality of Red Blood Cells |
| title_full_unstemmed | The Effect of Pre-Storage Irradiation Blood on Quality of Red Blood Cells |
| title_short | The Effect of Pre-Storage Irradiation Blood on Quality of Red Blood Cells |
| title_sort | effect of pre storage irradiation blood on quality of red blood cells |
| topic | Red blood cells, Gamma irradiation, Storage lesions, Transfusion-associated graft versus host disease (TA-GVHD) |
| url | https://ijhoscr.tums.ac.ir/index.php/ijhoscr/article/view/1254 |
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