Antioxidant, cytotoxic and apoptotic activities of the rhizome of Zingiber zerumbet Linn. in Ehrlich ascites carcinoma bearing Swiss albino mice
Abstract Due to having a long history of traditional uses as a functional food, Zingiber zerumbet was selected here to explore the inherent antioxidant and antineoplastic activities of methanolic extract of its rhizome (MEZZR) against Ehrlich ascites carcinoma (EAC) cells. The rich polyphenol contai...
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Nature Portfolio
2022-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-15498-8 |
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| author | Hanif Ali Rumana Yesmin Hasi Majidul Islam Md Shajedul Haque Mustfa F. Alkhanani Atiah H. Almalki Shafiul Haque R. Z. Sayyed Tanzima Yeasmin |
| author_facet | Hanif Ali Rumana Yesmin Hasi Majidul Islam Md Shajedul Haque Mustfa F. Alkhanani Atiah H. Almalki Shafiul Haque R. Z. Sayyed Tanzima Yeasmin |
| author_sort | Hanif Ali |
| collection | DOAJ |
| description | Abstract Due to having a long history of traditional uses as a functional food, Zingiber zerumbet was selected here to explore the inherent antioxidant and antineoplastic activities of methanolic extract of its rhizome (MEZZR) against Ehrlich ascites carcinoma (EAC) cells. The rich polyphenol containing MEZZR showed a marked DPPH, ABTS, nitric oxide radicals and lipid peroxidation inhibition activity with an IC50 of 3.43 ± 1.25, 11.38 ± 1.39, 23.12 ± 3.39 and 16.47 ± 1.47 µg/ml, respectively, when compared to the standard catechin. In vivo, MEZZR significantly inhibited EAC cell growth, decreased body weight gain, increased life span and restored the altered hematological characteristics of EAC-bearing mice. Moreover, MEZZR induced nuclear condensation and fragmentation, which are notable features of apoptosis as observed by fluorescence microscopy after staining EAC cells of MEZZR-treated mice with Hoechst 33342. Additionally, in vitro, the cell growth inhibition caused by the MEZZR in MTT assay, was remarkably decreased in the presence of caspase-3, -8 and -9 inhibitors. This study thus suggests that MEZZR may possess promising antiproliferative efficacy against EAC cells by inducing cell apoptosis. |
| first_indexed | 2024-12-10T23:23:21Z |
| format | Article |
| id | doaj.art-bedd948d9e6542768724814e9b80f728 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-10T23:23:21Z |
| publishDate | 2022-07-01 |
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| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-bedd948d9e6542768724814e9b80f7282022-12-22T01:29:39ZengNature PortfolioScientific Reports2045-23222022-07-0112111110.1038/s41598-022-15498-8Antioxidant, cytotoxic and apoptotic activities of the rhizome of Zingiber zerumbet Linn. in Ehrlich ascites carcinoma bearing Swiss albino miceHanif Ali0Rumana Yesmin Hasi1Majidul Islam2Md Shajedul Haque3Mustfa F. Alkhanani4Atiah H. Almalki5Shafiul Haque6R. Z. Sayyed7Tanzima Yeasmin8Department of Biochemistry and Molecular Biology, University of RajshahiDepartment of Biochemistry and Molecular Biology, University of RajshahiDepartment of Biochemistry and Molecular Biology, University of RajshahiDepartment of Biochemistry and Molecular Biology, University of RajshahiBiology Department, College of Sciences, University of Hafr Al BatinDepartment of Pharmaceutical Chemistry, College of Pharmacy, Taif UniversityResearch and Scientific Studies Unit, College of Nursing and Allied Health Sciences, Jazan UniversityDepartment of Microbiology, PSGVP Mandal’s S I Patil Arts, G B Patel Science and STKVS Commerce CollegeDepartment of Biochemistry and Molecular Biology, University of RajshahiAbstract Due to having a long history of traditional uses as a functional food, Zingiber zerumbet was selected here to explore the inherent antioxidant and antineoplastic activities of methanolic extract of its rhizome (MEZZR) against Ehrlich ascites carcinoma (EAC) cells. The rich polyphenol containing MEZZR showed a marked DPPH, ABTS, nitric oxide radicals and lipid peroxidation inhibition activity with an IC50 of 3.43 ± 1.25, 11.38 ± 1.39, 23.12 ± 3.39 and 16.47 ± 1.47 µg/ml, respectively, when compared to the standard catechin. In vivo, MEZZR significantly inhibited EAC cell growth, decreased body weight gain, increased life span and restored the altered hematological characteristics of EAC-bearing mice. Moreover, MEZZR induced nuclear condensation and fragmentation, which are notable features of apoptosis as observed by fluorescence microscopy after staining EAC cells of MEZZR-treated mice with Hoechst 33342. Additionally, in vitro, the cell growth inhibition caused by the MEZZR in MTT assay, was remarkably decreased in the presence of caspase-3, -8 and -9 inhibitors. This study thus suggests that MEZZR may possess promising antiproliferative efficacy against EAC cells by inducing cell apoptosis.https://doi.org/10.1038/s41598-022-15498-8 |
| spellingShingle | Hanif Ali Rumana Yesmin Hasi Majidul Islam Md Shajedul Haque Mustfa F. Alkhanani Atiah H. Almalki Shafiul Haque R. Z. Sayyed Tanzima Yeasmin Antioxidant, cytotoxic and apoptotic activities of the rhizome of Zingiber zerumbet Linn. in Ehrlich ascites carcinoma bearing Swiss albino mice Scientific Reports |
| title | Antioxidant, cytotoxic and apoptotic activities of the rhizome of Zingiber zerumbet Linn. in Ehrlich ascites carcinoma bearing Swiss albino mice |
| title_full | Antioxidant, cytotoxic and apoptotic activities of the rhizome of Zingiber zerumbet Linn. in Ehrlich ascites carcinoma bearing Swiss albino mice |
| title_fullStr | Antioxidant, cytotoxic and apoptotic activities of the rhizome of Zingiber zerumbet Linn. in Ehrlich ascites carcinoma bearing Swiss albino mice |
| title_full_unstemmed | Antioxidant, cytotoxic and apoptotic activities of the rhizome of Zingiber zerumbet Linn. in Ehrlich ascites carcinoma bearing Swiss albino mice |
| title_short | Antioxidant, cytotoxic and apoptotic activities of the rhizome of Zingiber zerumbet Linn. in Ehrlich ascites carcinoma bearing Swiss albino mice |
| title_sort | antioxidant cytotoxic and apoptotic activities of the rhizome of zingiber zerumbet linn in ehrlich ascites carcinoma bearing swiss albino mice |
| url | https://doi.org/10.1038/s41598-022-15498-8 |
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