Synthesis, physico-chemical characterization, antimicrobial activity and toxicological features of AgZnO nanoparticles
Silver-zinc oxide nanoparticles (AgZnO NPs) were chemically synthesized by the deposition of Ag NPs on the surface of ZnO NPs using silver nitrate, three types of anionic polyelectrolytes and citric acid as reagents. The Wavelength Dispersive X-ray Fluorescence (WDXRF) spectrometry of AgZnO NPs re...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2020-02-01
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| Series: | Arabian Journal of Chemistry |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535219300759 |
| _version_ | 1819020148435058688 |
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| author | Liliana Burlibaşa Mariana Carmen Chifiriuc Magdalena Valentina Lungu Eduard Marius Lungulescu Sorina Mitrea Gabriela Sbarcea Marcela Popa Luminiţa Măruţescu Nicoleta Constantin Coralia Bleotu Anca Hermenean |
| author_facet | Liliana Burlibaşa Mariana Carmen Chifiriuc Magdalena Valentina Lungu Eduard Marius Lungulescu Sorina Mitrea Gabriela Sbarcea Marcela Popa Luminiţa Măruţescu Nicoleta Constantin Coralia Bleotu Anca Hermenean |
| author_sort | Liliana Burlibaşa |
| collection | DOAJ |
| description | Silver-zinc oxide nanoparticles (AgZnO NPs) were chemically synthesized by the deposition of Ag NPs on the surface of ZnO NPs using silver nitrate, three types of anionic polyelectrolytes and citric acid as reagents. The Wavelength Dispersive X-ray Fluorescence (WDXRF) spectrometry of AgZnO NPs revealed 0.41–0.69 wt% Ag, and balance ZnO. The existence of Ag NPs on the surface of ZnO NPs with hexagonal wurtzite structure was highlighted by X-ray Diffraction (XRD) analysis, scanning electron microscopy (SEM), and Ultraviolet–Visible (UV–Vis) spectroscopy. The diffuse reflectance absorption of AgZnO NPs in the visible light region increased with the increase of Ag NPs content. The Fourier Transform Infrared (FTIR) spectrometry revealed no chemical bonding between Ag NPs and ZnO NPs and confirmed the presence of functional groups characteristic to ZnO and carboxylic acid salts. The newly synthesized AgZnO NPs displayed antimicrobial activity against all the tested medically relevant pathogens, with minimal (biofilm) inhibitory concentrations ranging from 1.875 mg/mL to 7.5 mg/mL. Although the in vitro genotoxicity assay revealed a relatively high micronuclei index, the in vivo micronucleus (MN) test revealed a low MN frequency in animals treated with AgZnO NPs. The histopathological analysis revealed non-significant structural changes of the hepatic parenchyma, renal cortex and intestinal mucosa and minimal inflammatory reactions. The AgZnO NPs administration induced TUNEL positive nuclei of Kupffer cells in the liver parenchyma. The present study shows that the newly synthesized AgZnO NPs are active against planktonic and adherent microorganisms and could be exploited to develop novel antimicrobial strategies for the biotechnology and biomedical fields. Easy scalability of the developed chemical synthesis is a major advantage in producing large batches of AgZnO NPs with reproducible properties. Keywords: Chemically synthesized AgZnO nanoparticles, Anionic polyelectrolytes, Medically relevant pathogens, Antimicrobial activity, Genotoxicity, Immunohistopathology |
| first_indexed | 2024-12-21T03:46:36Z |
| format | Article |
| id | doaj.art-bee0321543344269ab0da47a268a7659 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-12-21T03:46:36Z |
| publishDate | 2020-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-bee0321543344269ab0da47a268a76592022-12-21T19:17:04ZengElsevierArabian Journal of Chemistry1878-53522020-02-0113241804197Synthesis, physico-chemical characterization, antimicrobial activity and toxicological features of AgZnO nanoparticlesLiliana Burlibaşa0Mariana Carmen Chifiriuc1Magdalena Valentina Lungu2Eduard Marius Lungulescu3Sorina Mitrea4Gabriela Sbarcea5Marcela Popa6Luminiţa Măruţescu7Nicoleta Constantin8Coralia Bleotu9Anca Hermenean10University of Bucharest, Faculty of Biology, 1-3 Portocalilor Way, 060101 Bucharest, Romania; Research Institute of the University of Bucharest, ICUB, 91-95 Splaiul Independenţei, 050095 Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, 1-3 Portocalilor Way, 060101 Bucharest, Romania; Research Institute of the University of Bucharest, ICUB, 91-95 Splaiul Independenţei, 050095 Bucharest, RomaniaNational Institute for Research and Development in Electrical Engineering ICPE-CA, Department of Metallic, Composite and Polymeric Materials & Department of Characterization of Materials and Products for Electrical and Energy Engineering, 313 Splaiul Unirii Street, 030138 Bucharest, Romania; Corresponding author.National Institute for Research and Development in Electrical Engineering ICPE-CA, Department of Metallic, Composite and Polymeric Materials & Department of Characterization of Materials and Products for Electrical and Energy Engineering, 313 Splaiul Unirii Street, 030138 Bucharest, RomaniaNational Institute for Research and Development in Electrical Engineering ICPE-CA, Department of Metallic, Composite and Polymeric Materials & Department of Characterization of Materials and Products for Electrical and Energy Engineering, 313 Splaiul Unirii Street, 030138 Bucharest, RomaniaNational Institute for Research and Development in Electrical Engineering ICPE-CA, Department of Metallic, Composite and Polymeric Materials & Department of Characterization of Materials and Products for Electrical and Energy Engineering, 313 Splaiul Unirii Street, 030138 Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, 1-3 Portocalilor Way, 060101 Bucharest, Romania; Research Institute of the University of Bucharest, ICUB, 91-95 Splaiul Independenţei, 050095 Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, 1-3 Portocalilor Way, 060101 Bucharest, Romania; Research Institute of the University of Bucharest, ICUB, 91-95 Splaiul Independenţei, 050095 Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, 1-3 Portocalilor Way, 060101 Bucharest, Romania; Research Institute of the University of Bucharest, ICUB, 91-95 Splaiul Independenţei, 050095 Bucharest, RomaniaUniversity of Bucharest, Faculty of Biology, 1-3 Portocalilor Way, 060101 Bucharest, Romania; “Ştefan S. Nicolau” Institute of Virology, 285 Sos. Mihai Bravu, 030304 Bucharest, Romania“Vasile Goldiş” Western University of Arad, Faculty of Medicine, Department of Histology, 1 Feleacului, 310396 Arad, Romania; University Politehnica of Bucharest, Faculty of Applied Chemistry and Materials Science, Department of Science and Engineering of Oxide Materials and Nanomaterials, 1-7 Gh. Polizu Street, 011061 Bucharest, RomaniaSilver-zinc oxide nanoparticles (AgZnO NPs) were chemically synthesized by the deposition of Ag NPs on the surface of ZnO NPs using silver nitrate, three types of anionic polyelectrolytes and citric acid as reagents. The Wavelength Dispersive X-ray Fluorescence (WDXRF) spectrometry of AgZnO NPs revealed 0.41–0.69 wt% Ag, and balance ZnO. The existence of Ag NPs on the surface of ZnO NPs with hexagonal wurtzite structure was highlighted by X-ray Diffraction (XRD) analysis, scanning electron microscopy (SEM), and Ultraviolet–Visible (UV–Vis) spectroscopy. The diffuse reflectance absorption of AgZnO NPs in the visible light region increased with the increase of Ag NPs content. The Fourier Transform Infrared (FTIR) spectrometry revealed no chemical bonding between Ag NPs and ZnO NPs and confirmed the presence of functional groups characteristic to ZnO and carboxylic acid salts. The newly synthesized AgZnO NPs displayed antimicrobial activity against all the tested medically relevant pathogens, with minimal (biofilm) inhibitory concentrations ranging from 1.875 mg/mL to 7.5 mg/mL. Although the in vitro genotoxicity assay revealed a relatively high micronuclei index, the in vivo micronucleus (MN) test revealed a low MN frequency in animals treated with AgZnO NPs. The histopathological analysis revealed non-significant structural changes of the hepatic parenchyma, renal cortex and intestinal mucosa and minimal inflammatory reactions. The AgZnO NPs administration induced TUNEL positive nuclei of Kupffer cells in the liver parenchyma. The present study shows that the newly synthesized AgZnO NPs are active against planktonic and adherent microorganisms and could be exploited to develop novel antimicrobial strategies for the biotechnology and biomedical fields. Easy scalability of the developed chemical synthesis is a major advantage in producing large batches of AgZnO NPs with reproducible properties. Keywords: Chemically synthesized AgZnO nanoparticles, Anionic polyelectrolytes, Medically relevant pathogens, Antimicrobial activity, Genotoxicity, Immunohistopathologyhttp://www.sciencedirect.com/science/article/pii/S1878535219300759 |
| spellingShingle | Liliana Burlibaşa Mariana Carmen Chifiriuc Magdalena Valentina Lungu Eduard Marius Lungulescu Sorina Mitrea Gabriela Sbarcea Marcela Popa Luminiţa Măruţescu Nicoleta Constantin Coralia Bleotu Anca Hermenean Synthesis, physico-chemical characterization, antimicrobial activity and toxicological features of AgZnO nanoparticles Arabian Journal of Chemistry |
| title | Synthesis, physico-chemical characterization, antimicrobial activity and toxicological features of AgZnO nanoparticles |
| title_full | Synthesis, physico-chemical characterization, antimicrobial activity and toxicological features of AgZnO nanoparticles |
| title_fullStr | Synthesis, physico-chemical characterization, antimicrobial activity and toxicological features of AgZnO nanoparticles |
| title_full_unstemmed | Synthesis, physico-chemical characterization, antimicrobial activity and toxicological features of AgZnO nanoparticles |
| title_short | Synthesis, physico-chemical characterization, antimicrobial activity and toxicological features of AgZnO nanoparticles |
| title_sort | synthesis physico chemical characterization antimicrobial activity and toxicological features of agzno nanoparticles |
| url | http://www.sciencedirect.com/science/article/pii/S1878535219300759 |
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