Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles
Bottom-up processing of nanobiomaterials enables the creation of a variety of macroscopic structures in natural systems. Here, we use optical means to produce macroscopic-assembled structures of nanoparticles (NPs) from protein molecules by using light-induced bubble (LIB) generation under asymmetri...
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Format: | Article |
Language: | English |
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AIP Publishing LLC
2019-01-01
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Series: | APL Photonics |
Online Access: | http://dx.doi.org/10.1063/1.5079306 |
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author | Mayu Ueda Yushi Nishimura Mamoru Tamura Syoji Ito Shiho Tokonami Takuya Iida |
author_facet | Mayu Ueda Yushi Nishimura Mamoru Tamura Syoji Ito Shiho Tokonami Takuya Iida |
author_sort | Mayu Ueda |
collection | DOAJ |
description | Bottom-up processing of nanobiomaterials enables the creation of a variety of macroscopic structures in natural systems. Here, we use optical means to produce macroscopic-assembled structures of nanoparticles (NPs) from protein molecules by using light-induced bubble (LIB) generation under asymmetric pressure-driven flow in a microchannel. The broadband optical response of assembled NPs facilitates the application of photon pressure and photothermal convection when irradiated by using an infrared laser. The presence of a large amount of protein allows the generation of a vast number of stable LIBs from optically assembled metallic NP-fixed beads (MNFBs). In the case of more diluted albumin solutions, the shrinking of a single LIB can cause the aggregation of MNFBs via fg-level albumin (3.4 fg in the observation region), like a microscale bubblegum. The size of the resulting aggregate can be controlled by changing the concentration of protein. These findings can be used to devise production methods not only for broadband optical nanocomposites but also for label-free methods to detect an extremely small amount of protein. |
first_indexed | 2024-12-13T12:29:42Z |
format | Article |
id | doaj.art-bee650b34615490ea85a11040f4f48d8 |
institution | Directory Open Access Journal |
issn | 2378-0967 |
language | English |
last_indexed | 2024-12-13T12:29:42Z |
publishDate | 2019-01-01 |
publisher | AIP Publishing LLC |
record_format | Article |
series | APL Photonics |
spelling | doaj.art-bee650b34615490ea85a11040f4f48d82022-12-21T23:46:04ZengAIP Publishing LLCAPL Photonics2378-09672019-01-0141010802010802-610.1063/1.5079306001901APPMicroflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubblesMayu Ueda0Yushi Nishimura1Mamoru Tamura2Syoji Ito3Shiho Tokonami4Takuya Iida5Department of Physical Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8570, JapanDivision of Molecular Materials Science, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, JapanDepartment of Physical Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8570, JapanDivision of Frontier Materials Science, Graduate School of Engineering Science, Osaka University, 1-3, Machikaneyama-cho, Toyonaka, Osaka 560-8531, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Sakai 599-8570, JapanDepartment of Physical Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8570, JapanBottom-up processing of nanobiomaterials enables the creation of a variety of macroscopic structures in natural systems. Here, we use optical means to produce macroscopic-assembled structures of nanoparticles (NPs) from protein molecules by using light-induced bubble (LIB) generation under asymmetric pressure-driven flow in a microchannel. The broadband optical response of assembled NPs facilitates the application of photon pressure and photothermal convection when irradiated by using an infrared laser. The presence of a large amount of protein allows the generation of a vast number of stable LIBs from optically assembled metallic NP-fixed beads (MNFBs). In the case of more diluted albumin solutions, the shrinking of a single LIB can cause the aggregation of MNFBs via fg-level albumin (3.4 fg in the observation region), like a microscale bubblegum. The size of the resulting aggregate can be controlled by changing the concentration of protein. These findings can be used to devise production methods not only for broadband optical nanocomposites but also for label-free methods to detect an extremely small amount of protein.http://dx.doi.org/10.1063/1.5079306 |
spellingShingle | Mayu Ueda Yushi Nishimura Mamoru Tamura Syoji Ito Shiho Tokonami Takuya Iida Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles APL Photonics |
title | Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles |
title_full | Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles |
title_fullStr | Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles |
title_full_unstemmed | Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles |
title_short | Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles |
title_sort | microflow mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light induced bubbles |
url | http://dx.doi.org/10.1063/1.5079306 |
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