Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles

Bottom-up processing of nanobiomaterials enables the creation of a variety of macroscopic structures in natural systems. Here, we use optical means to produce macroscopic-assembled structures of nanoparticles (NPs) from protein molecules by using light-induced bubble (LIB) generation under asymmetri...

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Main Authors: Mayu Ueda, Yushi Nishimura, Mamoru Tamura, Syoji Ito, Shiho Tokonami, Takuya Iida
Format: Article
Language:English
Published: AIP Publishing LLC 2019-01-01
Series:APL Photonics
Online Access:http://dx.doi.org/10.1063/1.5079306
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author Mayu Ueda
Yushi Nishimura
Mamoru Tamura
Syoji Ito
Shiho Tokonami
Takuya Iida
author_facet Mayu Ueda
Yushi Nishimura
Mamoru Tamura
Syoji Ito
Shiho Tokonami
Takuya Iida
author_sort Mayu Ueda
collection DOAJ
description Bottom-up processing of nanobiomaterials enables the creation of a variety of macroscopic structures in natural systems. Here, we use optical means to produce macroscopic-assembled structures of nanoparticles (NPs) from protein molecules by using light-induced bubble (LIB) generation under asymmetric pressure-driven flow in a microchannel. The broadband optical response of assembled NPs facilitates the application of photon pressure and photothermal convection when irradiated by using an infrared laser. The presence of a large amount of protein allows the generation of a vast number of stable LIBs from optically assembled metallic NP-fixed beads (MNFBs). In the case of more diluted albumin solutions, the shrinking of a single LIB can cause the aggregation of MNFBs via fg-level albumin (3.4 fg in the observation region), like a microscale bubblegum. The size of the resulting aggregate can be controlled by changing the concentration of protein. These findings can be used to devise production methods not only for broadband optical nanocomposites but also for label-free methods to detect an extremely small amount of protein.
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spelling doaj.art-bee650b34615490ea85a11040f4f48d82022-12-21T23:46:04ZengAIP Publishing LLCAPL Photonics2378-09672019-01-0141010802010802-610.1063/1.5079306001901APPMicroflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubblesMayu Ueda0Yushi Nishimura1Mamoru Tamura2Syoji Ito3Shiho Tokonami4Takuya Iida5Department of Physical Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8570, JapanDivision of Molecular Materials Science, Graduate School of Science, Osaka City University, Sumiyoshi-ku, Osaka 558-8585, JapanDepartment of Physical Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8570, JapanDivision of Frontier Materials Science, Graduate School of Engineering Science, Osaka University, 1-3, Machikaneyama-cho, Toyonaka, Osaka 560-8531, JapanDepartment of Applied Chemistry, Graduate School of Engineering, Osaka Prefecture University, Sakai 599-8570, JapanDepartment of Physical Science, Graduate School of Science, Osaka Prefecture University, Sakai, Osaka 599-8570, JapanBottom-up processing of nanobiomaterials enables the creation of a variety of macroscopic structures in natural systems. Here, we use optical means to produce macroscopic-assembled structures of nanoparticles (NPs) from protein molecules by using light-induced bubble (LIB) generation under asymmetric pressure-driven flow in a microchannel. The broadband optical response of assembled NPs facilitates the application of photon pressure and photothermal convection when irradiated by using an infrared laser. The presence of a large amount of protein allows the generation of a vast number of stable LIBs from optically assembled metallic NP-fixed beads (MNFBs). In the case of more diluted albumin solutions, the shrinking of a single LIB can cause the aggregation of MNFBs via fg-level albumin (3.4 fg in the observation region), like a microscale bubblegum. The size of the resulting aggregate can be controlled by changing the concentration of protein. These findings can be used to devise production methods not only for broadband optical nanocomposites but also for label-free methods to detect an extremely small amount of protein.http://dx.doi.org/10.1063/1.5079306
spellingShingle Mayu Ueda
Yushi Nishimura
Mamoru Tamura
Syoji Ito
Shiho Tokonami
Takuya Iida
Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles
APL Photonics
title Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles
title_full Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles
title_fullStr Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles
title_full_unstemmed Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles
title_short Microflow-mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light-induced bubbles
title_sort microflow mediated optical assembly of nanoparticles with femtogram protein via shrinkage of light induced bubbles
url http://dx.doi.org/10.1063/1.5079306
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