Nanocomposite based on Gd2O3 nanoparticles and drug 5-fluorouracil as potential theranostic nano-cargo system
We have prepared silica matrix with hexagonal symmetry of pores (SBA-15) and loaded it with anticancer drug 5-Fluorouracil (5-FU) to promote it as a drug delivery system. Gd2O3 nanoparticles were incorporated into the matrix to enhance nanosystems applicability as contrast agent for MRI, thus enable...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Heliyon |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023081835 |
| _version_ | 1827616108614516736 |
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| author | Jaroslava Szűcsová Adriana Zeleňáková Eva Beňová Ľuboš Nagy Martin Orendáč Veronika Huntošová Mária Šoltésová Jaroslav Kohout Vít Herynek Vladimír Zeleňák |
| author_facet | Jaroslava Szűcsová Adriana Zeleňáková Eva Beňová Ľuboš Nagy Martin Orendáč Veronika Huntošová Mária Šoltésová Jaroslav Kohout Vít Herynek Vladimír Zeleňák |
| author_sort | Jaroslava Szűcsová |
| collection | DOAJ |
| description | We have prepared silica matrix with hexagonal symmetry of pores (SBA-15) and loaded it with anticancer drug 5-Fluorouracil (5-FU) to promote it as a drug delivery system. Gd2O3 nanoparticles were incorporated into the matrix to enhance nanosystems applicability as contrast agent for MRI, thus enabled this nanocomposite to be used as multifunctional nano-based therapeutic agent. Drug release profile was obtained by UV-VIS spectroscopy, and it indicates the prolongated release of 5-FU during the first hours and the total release after 5 h. The cytotoxicity tests using MTT-assay, fluorescent microscopy, bright-field microscopy, and flow cytometry were carried out using human glioma U87 MG cells and SK BR 3 cells. The nanocomposite with anticancer drug (Gd2O3/SBA-15/5FU) showed toxic behaviour towards studied cells, unlike nanocomposite without drug (Gd2O3/SBA-15) that was non-toxic. Our drug delivery system was designed to minimalize negative effect of Gd3+ ions at magnetic resonance imaging and drug 5-FU on healthy cells due to their encapsulation into biocompatible silica matrix, so the Gd3+ ions are more stable (in comparison to chelates), lower therapeutic dose of 5-FU is needed and its prolongated release from silica pores was confirmed. Very good T1 contrast in MR images was observed even at low concentrations, thus this nanosystem can be potentially used as contrast imaging agent. |
| first_indexed | 2024-03-09T09:21:29Z |
| format | Article |
| id | doaj.art-bee8bda3d34b4a23835552a6fc1738d8 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-09T09:21:29Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-bee8bda3d34b4a23835552a6fc1738d82023-12-02T07:01:02ZengElsevierHeliyon2405-84402023-11-01911e20975Nanocomposite based on Gd2O3 nanoparticles and drug 5-fluorouracil as potential theranostic nano-cargo systemJaroslava Szűcsová0Adriana Zeleňáková1Eva Beňová2Ľuboš Nagy3Martin Orendáč4Veronika Huntošová5Mária Šoltésová6Jaroslav Kohout7Vít Herynek8Vladimír Zeleňák9Institute of Physics, P. J. Šafárik University, Park Angelinum 9, 040 01 Kosice, SlovakiaInstitute of Physics, P. J. Šafárik University, Park Angelinum 9, 040 01 Kosice, Slovakia; Corresponding authorInstitute of Chemistry, P. J. Šafárik University, Moyzesova 11, 040 01 Kosice, SlovakiaInstitute of Physics, P. J. Šafárik University, Park Angelinum 9, 040 01 Kosice, SlovakiaInstitute of Physics, P. J. Šafárik University, Park Angelinum 9, 040 01 Kosice, Slovakia; Department of Solid State Engineering, University of Chemistry & Technology, Technická 5, 166 28 Prague, Czech RepublicCenter for Interdisciplinary Biosciences, P. J. Šafárik University, Jesenná 5, 040 01 Kosice, SlovakiaDepartment of Low Temperature Physics, Faculty of Mathematics and Physics, Charles University, V Holešovičkách 2, 182 00 Prague, Czech RepublicDepartment of Low Temperature Physics, Faculty of Mathematics and Physics, Charles University, V Holešovičkách 2, 182 00 Prague, Czech RepublicFirst Faculty of Medicine, Charles University, Center for Advanced Preclinical Imaging (CAPI), Salmovská 3, 120 00 Prague, Czech RepublicInstitute of Chemistry, P. J. Šafárik University, Moyzesova 11, 040 01 Kosice, SlovakiaWe have prepared silica matrix with hexagonal symmetry of pores (SBA-15) and loaded it with anticancer drug 5-Fluorouracil (5-FU) to promote it as a drug delivery system. Gd2O3 nanoparticles were incorporated into the matrix to enhance nanosystems applicability as contrast agent for MRI, thus enabled this nanocomposite to be used as multifunctional nano-based therapeutic agent. Drug release profile was obtained by UV-VIS spectroscopy, and it indicates the prolongated release of 5-FU during the first hours and the total release after 5 h. The cytotoxicity tests using MTT-assay, fluorescent microscopy, bright-field microscopy, and flow cytometry were carried out using human glioma U87 MG cells and SK BR 3 cells. The nanocomposite with anticancer drug (Gd2O3/SBA-15/5FU) showed toxic behaviour towards studied cells, unlike nanocomposite without drug (Gd2O3/SBA-15) that was non-toxic. Our drug delivery system was designed to minimalize negative effect of Gd3+ ions at magnetic resonance imaging and drug 5-FU on healthy cells due to their encapsulation into biocompatible silica matrix, so the Gd3+ ions are more stable (in comparison to chelates), lower therapeutic dose of 5-FU is needed and its prolongated release from silica pores was confirmed. Very good T1 contrast in MR images was observed even at low concentrations, thus this nanosystem can be potentially used as contrast imaging agent.http://www.sciencedirect.com/science/article/pii/S2405844023081835mesoporous silicaGd2O3 nanoparticlesdrug deliveryMRI5-Fluorouracil |
| spellingShingle | Jaroslava Szűcsová Adriana Zeleňáková Eva Beňová Ľuboš Nagy Martin Orendáč Veronika Huntošová Mária Šoltésová Jaroslav Kohout Vít Herynek Vladimír Zeleňák Nanocomposite based on Gd2O3 nanoparticles and drug 5-fluorouracil as potential theranostic nano-cargo system Heliyon mesoporous silica Gd2O3 nanoparticles drug delivery MRI 5-Fluorouracil |
| title | Nanocomposite based on Gd2O3 nanoparticles and drug 5-fluorouracil as potential theranostic nano-cargo system |
| title_full | Nanocomposite based on Gd2O3 nanoparticles and drug 5-fluorouracil as potential theranostic nano-cargo system |
| title_fullStr | Nanocomposite based on Gd2O3 nanoparticles and drug 5-fluorouracil as potential theranostic nano-cargo system |
| title_full_unstemmed | Nanocomposite based on Gd2O3 nanoparticles and drug 5-fluorouracil as potential theranostic nano-cargo system |
| title_short | Nanocomposite based on Gd2O3 nanoparticles and drug 5-fluorouracil as potential theranostic nano-cargo system |
| title_sort | nanocomposite based on gd2o3 nanoparticles and drug 5 fluorouracil as potential theranostic nano cargo system |
| topic | mesoporous silica Gd2O3 nanoparticles drug delivery MRI 5-Fluorouracil |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023081835 |
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