Generation of homozygous and heterozygous REEP1 knockout induced pluripotent stem cell lines by CRISPR/Cas9 gene editing
REEP1 is a transmembrane protein in the endoplasmic reticulum (ER) membrane that is involved in shaping and remodeling of the ER. Mutations in REEP1 cause SPG31, an autosomal dominant form of hereditary spastic paraplegia (HSP). Here we show the generation of a homozygous and a heterozygous REEP1 kn...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2024-06-01
|
| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S187350612400076X |
| _version_ | 1797262946121809920 |
|---|---|
| author | M. Korneck A. Leonhardt L. Schöls S. Hauser |
| author_facet | M. Korneck A. Leonhardt L. Schöls S. Hauser |
| author_sort | M. Korneck |
| collection | DOAJ |
| description | REEP1 is a transmembrane protein in the endoplasmic reticulum (ER) membrane that is involved in shaping and remodeling of the ER. Mutations in REEP1 cause SPG31, an autosomal dominant form of hereditary spastic paraplegia (HSP). Here we show the generation of a homozygous and a heterozygous REEP1 knockout induced pluripotent stem cell line suitable for in vitro disease modelling using the CRISPR/Cas9 editing system. |
| first_indexed | 2024-04-25T00:05:11Z |
| format | Article |
| id | doaj.art-bee96af1e4274692b156fe68ecded063 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-04-25T00:05:11Z |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-bee96af1e4274692b156fe68ecded0632024-03-14T06:14:21ZengElsevierStem Cell Research1873-50612024-06-0177103378Generation of homozygous and heterozygous REEP1 knockout induced pluripotent stem cell lines by CRISPR/Cas9 gene editingM. Korneck0A. Leonhardt1L. Schöls2S. Hauser3Hertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany; Graduate School of Cellular and Molecular Neuroscience, University of Tübingen, Tübingen, GermanyGerman Center for Neurodegenerative Diseases (DZNE), Tübingen, GermanyHertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; German Center for Neurodegenerative Diseases (DZNE), Tübingen, GermanyHertie Institute for Clinical Brain Research, University of Tübingen, Tübingen, Germany; German Center for Neurodegenerative Diseases (DZNE), Tübingen, Germany; Corresponding author.REEP1 is a transmembrane protein in the endoplasmic reticulum (ER) membrane that is involved in shaping and remodeling of the ER. Mutations in REEP1 cause SPG31, an autosomal dominant form of hereditary spastic paraplegia (HSP). Here we show the generation of a homozygous and a heterozygous REEP1 knockout induced pluripotent stem cell line suitable for in vitro disease modelling using the CRISPR/Cas9 editing system.http://www.sciencedirect.com/science/article/pii/S187350612400076X |
| spellingShingle | M. Korneck A. Leonhardt L. Schöls S. Hauser Generation of homozygous and heterozygous REEP1 knockout induced pluripotent stem cell lines by CRISPR/Cas9 gene editing Stem Cell Research |
| title | Generation of homozygous and heterozygous REEP1 knockout induced pluripotent stem cell lines by CRISPR/Cas9 gene editing |
| title_full | Generation of homozygous and heterozygous REEP1 knockout induced pluripotent stem cell lines by CRISPR/Cas9 gene editing |
| title_fullStr | Generation of homozygous and heterozygous REEP1 knockout induced pluripotent stem cell lines by CRISPR/Cas9 gene editing |
| title_full_unstemmed | Generation of homozygous and heterozygous REEP1 knockout induced pluripotent stem cell lines by CRISPR/Cas9 gene editing |
| title_short | Generation of homozygous and heterozygous REEP1 knockout induced pluripotent stem cell lines by CRISPR/Cas9 gene editing |
| title_sort | generation of homozygous and heterozygous reep1 knockout induced pluripotent stem cell lines by crispr cas9 gene editing |
| url | http://www.sciencedirect.com/science/article/pii/S187350612400076X |
| work_keys_str_mv | AT mkorneck generationofhomozygousandheterozygousreep1knockoutinducedpluripotentstemcelllinesbycrisprcas9geneediting AT aleonhardt generationofhomozygousandheterozygousreep1knockoutinducedpluripotentstemcelllinesbycrisprcas9geneediting AT lschols generationofhomozygousandheterozygousreep1knockoutinducedpluripotentstemcelllinesbycrisprcas9geneediting AT shauser generationofhomozygousandheterozygousreep1knockoutinducedpluripotentstemcelllinesbycrisprcas9geneediting |