5-Aminolevulinic acid regulates the immune response in LPS-stimulated RAW 264.7 macrophages
Abstract Background Macrophages are crucial players in a variety of inflammatory responses to environmental cues. However, it has been widely reported that macrophages cause chronic inflammation and are involved in a variety of diseases, such as obesity, diabetes, metabolic syndrome, and cancer. In...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2018-12-01
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| Series: | BMC Immunology |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12865-018-0277-5 |
| _version_ | 1818243647508840448 |
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| author | Yuta Sugiyama Yukari Hiraiwa Yuichiro Hagiya Motowo Nakajima Tohru Tanaka Shun-ichiro Ogura |
| author_facet | Yuta Sugiyama Yukari Hiraiwa Yuichiro Hagiya Motowo Nakajima Tohru Tanaka Shun-ichiro Ogura |
| author_sort | Yuta Sugiyama |
| collection | DOAJ |
| description | Abstract Background Macrophages are crucial players in a variety of inflammatory responses to environmental cues. However, it has been widely reported that macrophages cause chronic inflammation and are involved in a variety of diseases, such as obesity, diabetes, metabolic syndrome, and cancer. In this study, we report the suppressive effect of 5-aminolevulinic acid (ALA), via the HO-1-related system, on the immune response of the LPS-stimulated mouse macrophage cell line RAW264.7. Results RAW264.7 cells were treated with LPS with or without ALA, and proinflammatory mediator expression levels and phagocytic ability were assessed. ALA treatment resulted in the attenuation of iNOS and NO expression and the downregulation of proinflammatory cytokines (TNF-α, cyclooxygenase2, IL-1β, IL-6). In addition, ALA treatment did not affect the phagocytic ability of macrophages. To our knowledge, this study is the first to investigate the effect of ALA on macrophage function. Our findings suggest that ALA may have high potential as a novel anti-inflammatory agent. Conclusions In the present study, we showed that exogenous addition of ALA induces HO-1 and leads to the downregulation of NO and some proinflammatory cytokines. These findings support ALA as a promising anti-inflammatory agent. |
| first_indexed | 2024-12-12T14:04:27Z |
| format | Article |
| id | doaj.art-beeb1bb96d1f4dca96b3de0ff843e91f |
| institution | Directory Open Access Journal |
| issn | 1471-2172 |
| language | English |
| last_indexed | 2024-12-12T14:04:27Z |
| publishDate | 2018-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Immunology |
| spelling | doaj.art-beeb1bb96d1f4dca96b3de0ff843e91f2022-12-22T00:22:16ZengBMCBMC Immunology1471-21722018-12-011911910.1186/s12865-018-0277-55-Aminolevulinic acid regulates the immune response in LPS-stimulated RAW 264.7 macrophagesYuta Sugiyama0Yukari Hiraiwa1Yuichiro Hagiya2Motowo Nakajima3Tohru Tanaka4Shun-ichiro Ogura5School of Life Science and Technology, Tokyo Institute of TechnologySchool of Life Science and Technology, Tokyo Institute of TechnologySchool of Life Science and Technology, Tokyo Institute of TechnologySBI Pharma CO., LTD.SBI Pharma CO., LTD.School of Life Science and Technology, Tokyo Institute of TechnologyAbstract Background Macrophages are crucial players in a variety of inflammatory responses to environmental cues. However, it has been widely reported that macrophages cause chronic inflammation and are involved in a variety of diseases, such as obesity, diabetes, metabolic syndrome, and cancer. In this study, we report the suppressive effect of 5-aminolevulinic acid (ALA), via the HO-1-related system, on the immune response of the LPS-stimulated mouse macrophage cell line RAW264.7. Results RAW264.7 cells were treated with LPS with or without ALA, and proinflammatory mediator expression levels and phagocytic ability were assessed. ALA treatment resulted in the attenuation of iNOS and NO expression and the downregulation of proinflammatory cytokines (TNF-α, cyclooxygenase2, IL-1β, IL-6). In addition, ALA treatment did not affect the phagocytic ability of macrophages. To our knowledge, this study is the first to investigate the effect of ALA on macrophage function. Our findings suggest that ALA may have high potential as a novel anti-inflammatory agent. Conclusions In the present study, we showed that exogenous addition of ALA induces HO-1 and leads to the downregulation of NO and some proinflammatory cytokines. These findings support ALA as a promising anti-inflammatory agent.http://link.springer.com/article/10.1186/s12865-018-0277-55-Aminolevulinic acidMacrophageHeme oxygenase-1LPSAnti inflammation |
| spellingShingle | Yuta Sugiyama Yukari Hiraiwa Yuichiro Hagiya Motowo Nakajima Tohru Tanaka Shun-ichiro Ogura 5-Aminolevulinic acid regulates the immune response in LPS-stimulated RAW 264.7 macrophages BMC Immunology 5-Aminolevulinic acid Macrophage Heme oxygenase-1 LPS Anti inflammation |
| title | 5-Aminolevulinic acid regulates the immune response in LPS-stimulated RAW 264.7 macrophages |
| title_full | 5-Aminolevulinic acid regulates the immune response in LPS-stimulated RAW 264.7 macrophages |
| title_fullStr | 5-Aminolevulinic acid regulates the immune response in LPS-stimulated RAW 264.7 macrophages |
| title_full_unstemmed | 5-Aminolevulinic acid regulates the immune response in LPS-stimulated RAW 264.7 macrophages |
| title_short | 5-Aminolevulinic acid regulates the immune response in LPS-stimulated RAW 264.7 macrophages |
| title_sort | 5 aminolevulinic acid regulates the immune response in lps stimulated raw 264 7 macrophages |
| topic | 5-Aminolevulinic acid Macrophage Heme oxygenase-1 LPS Anti inflammation |
| url | http://link.springer.com/article/10.1186/s12865-018-0277-5 |
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