Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype
There is significant interest in the role of stem cells in cardiac regeneration, and yet little is known about how cardiac disease progression affects native cardiac stem cells in the human heart. In this brief report, cardiac mesenchymal stem cell-like cells (CMSCLC) from the right atria of a 21-ye...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-12-01
|
| Series: | Biomedicines |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2227-9059/10/12/3143 |
| _version_ | 1797461311839272960 |
|---|---|
| author | Rachel A. Oldershaw Gavin Richardson Phillippa Carling W. Andrew Owens David J. Lundy Annette Meeson |
| author_facet | Rachel A. Oldershaw Gavin Richardson Phillippa Carling W. Andrew Owens David J. Lundy Annette Meeson |
| author_sort | Rachel A. Oldershaw |
| collection | DOAJ |
| description | There is significant interest in the role of stem cells in cardiac regeneration, and yet little is known about how cardiac disease progression affects native cardiac stem cells in the human heart. In this brief report, cardiac mesenchymal stem cell-like cells (CMSCLC) from the right atria of a 21-year-old female patient with a bicuspid aortic valve and aortic stenosis (referred to as biscuspid aortic valve disease BAVD-CMSCLC), were compared with those of a 78-year-old female patient undergoing coronary artery bypass surgery (referred to as coronary artery disease CAD-CMSCLC). Cells were analyzed for expression of MSC markers, ability to form CFU-Fs, metabolic activity, cell cycle kinetics, expression of NANOG and p16, and telomere length. The cardiac-derived cells expressed MSC markers and were able to form CFU-Fs, with higher rate of formation in CAD-CMSCLCs. BAVD-CMSCLCs did not display normal MSC morphology, had a much lower cell doubling rate, and were less metabolically active than CAD-CMSCLCs. Cell cycle analysis revealed a population of BAVD-CMSCLC in G2/M phase, whereas the bulk of CAD-CMSCLC were in the G0/G1 phase. BAVD-CMSCLC had lower expression of NANOG and shorter telomere lengths, but higher expression of p16 compared with the CAD-CMSCLC. In conclusion, BAVD-CMSCLC have a prematurely aged phenotype compared with CAD-CMSCLC, despite originating from a younger patient. |
| first_indexed | 2024-03-09T17:18:33Z |
| format | Article |
| id | doaj.art-beeda38117114a2cb70fb9e64822e0b6 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-09T17:18:33Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-beeda38117114a2cb70fb9e64822e0b62023-11-24T13:27:36ZengMDPI AGBiomedicines2227-90592022-12-011012314310.3390/biomedicines10123143Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged PhenotypeRachel A. Oldershaw0Gavin Richardson1Phillippa Carling2W. Andrew Owens3David J. Lundy4Annette Meeson5Department of Musculoskeletal and Ageing Science, Institute of Life Course and Medical Sciences, Faculty of Health and Life Sciences, University of Liverpool, William Henry Duncan Building, 6 West Derby Street, Liverpool L7 8TX, UKNewcastle University Bioscience Institute, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UKNewcastle University Bioscience Institute, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UKNewcastle University Bioscience Institute, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UKGraduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, TaiwanNewcastle University Bioscience Institute, Newcastle University, International Centre for Life, Central Parkway, Newcastle upon Tyne NE1 3BZ, UKThere is significant interest in the role of stem cells in cardiac regeneration, and yet little is known about how cardiac disease progression affects native cardiac stem cells in the human heart. In this brief report, cardiac mesenchymal stem cell-like cells (CMSCLC) from the right atria of a 21-year-old female patient with a bicuspid aortic valve and aortic stenosis (referred to as biscuspid aortic valve disease BAVD-CMSCLC), were compared with those of a 78-year-old female patient undergoing coronary artery bypass surgery (referred to as coronary artery disease CAD-CMSCLC). Cells were analyzed for expression of MSC markers, ability to form CFU-Fs, metabolic activity, cell cycle kinetics, expression of NANOG and p16, and telomere length. The cardiac-derived cells expressed MSC markers and were able to form CFU-Fs, with higher rate of formation in CAD-CMSCLCs. BAVD-CMSCLCs did not display normal MSC morphology, had a much lower cell doubling rate, and were less metabolically active than CAD-CMSCLCs. Cell cycle analysis revealed a population of BAVD-CMSCLC in G2/M phase, whereas the bulk of CAD-CMSCLC were in the G0/G1 phase. BAVD-CMSCLC had lower expression of NANOG and shorter telomere lengths, but higher expression of p16 compared with the CAD-CMSCLC. In conclusion, BAVD-CMSCLC have a prematurely aged phenotype compared with CAD-CMSCLC, despite originating from a younger patient.https://www.mdpi.com/2227-9059/10/12/3143cardiac mesenchymal stem cell-like cellsbicuspid aortic valve diseasecoronary artery diseasemesenchymal stem cellsageingsenescence |
| spellingShingle | Rachel A. Oldershaw Gavin Richardson Phillippa Carling W. Andrew Owens David J. Lundy Annette Meeson Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype Biomedicines cardiac mesenchymal stem cell-like cells bicuspid aortic valve disease coronary artery disease mesenchymal stem cells ageing senescence |
| title | Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype |
| title_full | Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype |
| title_fullStr | Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype |
| title_full_unstemmed | Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype |
| title_short | Cardiac Mesenchymal Stem Cell-like Cells Derived from a Young Patient with Bicuspid Aortic Valve Disease Have a Prematurely Aged Phenotype |
| title_sort | cardiac mesenchymal stem cell like cells derived from a young patient with bicuspid aortic valve disease have a prematurely aged phenotype |
| topic | cardiac mesenchymal stem cell-like cells bicuspid aortic valve disease coronary artery disease mesenchymal stem cells ageing senescence |
| url | https://www.mdpi.com/2227-9059/10/12/3143 |
| work_keys_str_mv | AT rachelaoldershaw cardiacmesenchymalstemcelllikecellsderivedfromayoungpatientwithbicuspidaorticvalvediseasehaveaprematurelyagedphenotype AT gavinrichardson cardiacmesenchymalstemcelllikecellsderivedfromayoungpatientwithbicuspidaorticvalvediseasehaveaprematurelyagedphenotype AT phillippacarling cardiacmesenchymalstemcelllikecellsderivedfromayoungpatientwithbicuspidaorticvalvediseasehaveaprematurelyagedphenotype AT wandrewowens cardiacmesenchymalstemcelllikecellsderivedfromayoungpatientwithbicuspidaorticvalvediseasehaveaprematurelyagedphenotype AT davidjlundy cardiacmesenchymalstemcelllikecellsderivedfromayoungpatientwithbicuspidaorticvalvediseasehaveaprematurelyagedphenotype AT annettemeeson cardiacmesenchymalstemcelllikecellsderivedfromayoungpatientwithbicuspidaorticvalvediseasehaveaprematurelyagedphenotype |