Evaluation and validation on cellular immunoreactivity of Hantaan virus Gc
Objective To screen, identify and verify the immunoreactive epitopes on Hantaan virus glycoprotein C-terminal (HTNV Gc). Methods On the basis of predicting the major histocompatibility complex (MHC) gene in more than 98% of the global population, the affinity of 9-peptide and 15-peptide epitopes on...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Army Medical University
2023-10-01
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| Series: | 陆军军医大学学报 |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/html/202208161.htm |
| _version_ | 1827793646701772800 |
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| author | XIE Mingyang SUN Baozeng MA Sijie YANG Duan LUO Cheng |
| author_facet | XIE Mingyang SUN Baozeng MA Sijie YANG Duan LUO Cheng |
| author_sort | XIE Mingyang |
| collection | DOAJ |
| description | Objective To screen, identify and verify the immunoreactive epitopes on Hantaan virus glycoprotein C-terminal (HTNV Gc). Methods On the basis of predicting the major histocompatibility complex (MHC) gene in more than 98% of the global population, the affinity of 9-peptide and 15-peptide epitopes on HTNV Gc was systematically analyzed by bioinformatics cutting-edge algorithms such as Immune Epitope Database (IEDB) and Technical University of Denmark (DTU). Their immunogenicity was analyzed by using the Vaxijen tool, and the interspecies intraspecific conservation was analyzed with the Blastp tool. Bidirectional hierarchical clustering was employed to compare the cross-immunoreactivity of MHC-class Ⅰ and class Ⅱ restricted epitopes. Possible results of dominant epitopes docking with MHC were simulated by using molecular docking. Enzyme-linked immunospot (ELISpot) assay was performed to validate the results of computer analyses. Results After integrating the results of various bioinformatics algorithms, we obtained 8 9-peptide epitopes and 80 15-peptide epitopes with high affinity, intraspecific conservation and strong immunogenicity, including 8 human-mouse common dominant epitopes. Cluster analysis showed that H-2 genotype had certain similarity as HLA genotype. The results of molecular docking revealed that dominant epitopes had good docking with various MHC genotypes, which were consistent with the results of affinity analysis. The amount of IFN-γ secreted from the spleen cells was higher in the mice with dominant epitopes than those with non-dominant epitopes. Conclusion With the aids of various bioinformatics algorithms, we successfully screen out the dominant epitopes on HTNV Gc, and validate the cellular immunoreactivity of these epitopes through ELISpot assay.
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| first_indexed | 2024-03-11T18:21:25Z |
| format | Article |
| id | doaj.art-beefd3d427d14fa9b85d95f38a26afcc |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-03-11T18:21:25Z |
| publishDate | 2023-10-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-beefd3d427d14fa9b85d95f38a26afcc2023-10-15T10:57:24ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272023-10-0145192007201710.16016/j.2097-0927.202208161Evaluation and validation on cellular immunoreactivity of Hantaan virus GcXIE Mingyang0SUN Baozeng1MA Sijie2YANG Duan3LUO Cheng4Department of Immunology, Basic Medical Science Academy, Air Force Medical University, Xi'an, Shaanxi Province, 710032 Yingtan Detachment, Jiangxi Corps, Chinese People's Armed Police Force, Yingtan, Jiangxi Province, 335000, ChinaDepartment of Immunology, Basic Medical Science Academy, Air Force Medical University, Xi'an, Shaanxi Province, 710032Department of Immunology, Basic Medical Science Academy, Air Force Medical University, Xi'an, Shaanxi Province, 710032Department of Immunology, Basic Medical Science Academy, Air Force Medical University, Xi'an, Shaanxi Province, 710032Objective To screen, identify and verify the immunoreactive epitopes on Hantaan virus glycoprotein C-terminal (HTNV Gc). Methods On the basis of predicting the major histocompatibility complex (MHC) gene in more than 98% of the global population, the affinity of 9-peptide and 15-peptide epitopes on HTNV Gc was systematically analyzed by bioinformatics cutting-edge algorithms such as Immune Epitope Database (IEDB) and Technical University of Denmark (DTU). Their immunogenicity was analyzed by using the Vaxijen tool, and the interspecies intraspecific conservation was analyzed with the Blastp tool. Bidirectional hierarchical clustering was employed to compare the cross-immunoreactivity of MHC-class Ⅰ and class Ⅱ restricted epitopes. Possible results of dominant epitopes docking with MHC were simulated by using molecular docking. Enzyme-linked immunospot (ELISpot) assay was performed to validate the results of computer analyses. Results After integrating the results of various bioinformatics algorithms, we obtained 8 9-peptide epitopes and 80 15-peptide epitopes with high affinity, intraspecific conservation and strong immunogenicity, including 8 human-mouse common dominant epitopes. Cluster analysis showed that H-2 genotype had certain similarity as HLA genotype. The results of molecular docking revealed that dominant epitopes had good docking with various MHC genotypes, which were consistent with the results of affinity analysis. The amount of IFN-γ secreted from the spleen cells was higher in the mice with dominant epitopes than those with non-dominant epitopes. Conclusion With the aids of various bioinformatics algorithms, we successfully screen out the dominant epitopes on HTNV Gc, and validate the cellular immunoreactivity of these epitopes through ELISpot assay. http://aammt.tmmu.edu.cn/html/202208161.htmhantaan virusglycoproteinepitopesilico predictionimmunoreactivity |
| spellingShingle | XIE Mingyang SUN Baozeng MA Sijie YANG Duan LUO Cheng Evaluation and validation on cellular immunoreactivity of Hantaan virus Gc 陆军军医大学学报 hantaan virus glycoprotein epitope silico prediction immunoreactivity |
| title | Evaluation and validation on cellular immunoreactivity of Hantaan virus Gc |
| title_full | Evaluation and validation on cellular immunoreactivity of Hantaan virus Gc |
| title_fullStr | Evaluation and validation on cellular immunoreactivity of Hantaan virus Gc |
| title_full_unstemmed | Evaluation and validation on cellular immunoreactivity of Hantaan virus Gc |
| title_short | Evaluation and validation on cellular immunoreactivity of Hantaan virus Gc |
| title_sort | evaluation and validation on cellular immunoreactivity of hantaan virus gc |
| topic | hantaan virus glycoprotein epitope silico prediction immunoreactivity |
| url | http://aammt.tmmu.edu.cn/html/202208161.htm |
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