Disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long-term estrogen-deprived female mice
Hormone therapy (HT) has failed to improve learning and memory in postmenopausal women according to recent clinical studies; however, the reason for failure of HT in improving cognitive performance is unknown. In our research, we found cognitive flexibility was improved by 17β-Estradiol (E2) in mice...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-04-01
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| Series: | Brain Research Bulletin |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0361923022000168 |
| _version_ | 1797367849212182528 |
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| author | Fu Yang Yu-Jia Zhao Si-Jie Chen Ya-Ru Li Pei-Yue Yang Jing-Yu Qi Xin-Shang Wang Min Wang Xu-Bo Li Ban Feng Yu-Mei Wu Shui-Bing Liu Kun Zhang |
| author_facet | Fu Yang Yu-Jia Zhao Si-Jie Chen Ya-Ru Li Pei-Yue Yang Jing-Yu Qi Xin-Shang Wang Min Wang Xu-Bo Li Ban Feng Yu-Mei Wu Shui-Bing Liu Kun Zhang |
| author_sort | Fu Yang |
| collection | DOAJ |
| description | Hormone therapy (HT) has failed to improve learning and memory in postmenopausal women according to recent clinical studies; however, the reason for failure of HT in improving cognitive performance is unknown. In our research, we found cognitive flexibility was improved by 17β-Estradiol (E2) in mice 1 week after ovariectomy (OVXST), but not in mice 3 months after ovariectomy (OVXLT). Isobaric tags for relative and absolute quantitation (iTRAQ) revealed increased cannabinoid receptor interacting protein 1 (CNRIP1) in E2-treated OVXLT mice compared with E2-treated OVXST mice. Adeno-associated virus 2/9 (AAV2/9) delivery of Cnrip1 short-hairpin small interfering RNA (Cnrip1-shRNA) rescued the impaired cognitive flexibility in E2 treated OVXLT mice. This effect is dependent on CB1 function, which could be blocked by AM251—a CB1 antagonist. Our results indicated a new method to increasing cognitive flexibility in women receiving HT by disrupting CNRIP1. |
| first_indexed | 2024-03-08T17:23:02Z |
| format | Article |
| id | doaj.art-bef0ac33b63a4543b10e0a2032d5bd73 |
| institution | Directory Open Access Journal |
| issn | 1873-2747 |
| language | English |
| last_indexed | 2024-03-08T17:23:02Z |
| publishDate | 2022-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brain Research Bulletin |
| spelling | doaj.art-bef0ac33b63a4543b10e0a2032d5bd732024-01-03T04:11:08ZengElsevierBrain Research Bulletin1873-27472022-04-011817786Disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long-term estrogen-deprived female miceFu Yang0Yu-Jia Zhao1Si-Jie Chen2Ya-Ru Li3Pei-Yue Yang4Jing-Yu Qi5Xin-Shang Wang6Min Wang7Xu-Bo Li8Ban Feng9Yu-Mei Wu10Shui-Bing Liu11Kun Zhang12Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, ChinaSchool of Medicine, Xi'an Jiaotong University, Xi’an 710086, ChinaSchool of Medicine, Xi'an Jiaotong University, Xi’an 710086, ChinaDepartment of Clinical Pharmacy, Baotou Medical College, Baotou, Inner Mongolia 014060, ChinaDepartment of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, ChinaDepartment of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, China; School of Medicine, Xi'an Jiaotong University, Xi’an 710086, China; Department of Clinical Pharmacy, Baotou Medical College, Baotou, Inner Mongolia 014060, ChinaDepartment of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, China; Corresponding authors.Department of Pharmacology, School of Pharmacy, Fourth Military Medical University, Xi’an 710032, China; Corresponding authors.Hormone therapy (HT) has failed to improve learning and memory in postmenopausal women according to recent clinical studies; however, the reason for failure of HT in improving cognitive performance is unknown. In our research, we found cognitive flexibility was improved by 17β-Estradiol (E2) in mice 1 week after ovariectomy (OVXST), but not in mice 3 months after ovariectomy (OVXLT). Isobaric tags for relative and absolute quantitation (iTRAQ) revealed increased cannabinoid receptor interacting protein 1 (CNRIP1) in E2-treated OVXLT mice compared with E2-treated OVXST mice. Adeno-associated virus 2/9 (AAV2/9) delivery of Cnrip1 short-hairpin small interfering RNA (Cnrip1-shRNA) rescued the impaired cognitive flexibility in E2 treated OVXLT mice. This effect is dependent on CB1 function, which could be blocked by AM251—a CB1 antagonist. Our results indicated a new method to increasing cognitive flexibility in women receiving HT by disrupting CNRIP1.http://www.sciencedirect.com/science/article/pii/S0361923022000168Hormone therapyCognitive flexibilityCannabinoid receptor interacting protein 1Fear extinctionMedial prefrontal cortex |
| spellingShingle | Fu Yang Yu-Jia Zhao Si-Jie Chen Ya-Ru Li Pei-Yue Yang Jing-Yu Qi Xin-Shang Wang Min Wang Xu-Bo Li Ban Feng Yu-Mei Wu Shui-Bing Liu Kun Zhang Disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long-term estrogen-deprived female mice Brain Research Bulletin Hormone therapy Cognitive flexibility Cannabinoid receptor interacting protein 1 Fear extinction Medial prefrontal cortex |
| title | Disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long-term estrogen-deprived female mice |
| title_full | Disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long-term estrogen-deprived female mice |
| title_fullStr | Disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long-term estrogen-deprived female mice |
| title_full_unstemmed | Disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long-term estrogen-deprived female mice |
| title_short | Disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long-term estrogen-deprived female mice |
| title_sort | disrupting cannabinoid receptor interacting protein 1 rescues cognitive flexibility in long term estrogen deprived female mice |
| topic | Hormone therapy Cognitive flexibility Cannabinoid receptor interacting protein 1 Fear extinction Medial prefrontal cortex |
| url | http://www.sciencedirect.com/science/article/pii/S0361923022000168 |
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