THE EFFECT OF MICROENCAPSULATION AND POTATO STARCH ON THE SURVIVAL OF LACTOBACILLUS STRAINS

The effectiveness of encapsulating ofactive bacteria in releasing probiotics depends on cell survival. In this study, a prebiotic - potato starch was used to evaluate its influence on the viability of probiotics microcapsulated. Lactobacilluscasei, Lactobacillus rhamnosus and Lactobacillus plantaru...

Full description

Bibliographic Details
Main Authors: Liliana LUCA, Mircea OROIAN
Format: Article
Language:English
Published: Stefan cel Mare University of Suceava 2020-06-01
Series:Food and Environment Safety
Subjects:
Online Access:http://fia-old.usv.ro/fiajournal/index.php/FENS/article/view/719/647
_version_ 1818346082220900352
author Liliana LUCA
Mircea OROIAN
author_facet Liliana LUCA
Mircea OROIAN
author_sort Liliana LUCA
collection DOAJ
description The effectiveness of encapsulating ofactive bacteria in releasing probiotics depends on cell survival. In this study, a prebiotic - potato starch was used to evaluate its influence on the viability of probiotics microcapsulated. Lactobacilluscasei, Lactobacillus rhamnosus and Lactobacillus plantarumwas cultured for 16 hours and after that, the cells were added to an emulsion with 1.5% sodium alginate, supplemented with 1% prebiotics and encapsulated. Initial tests revealed that the initial culture concentration is not relevant for prebiotic supplementation. The testing of fresh microcapsules recorded a load of 3.31 × 10 ¹³ cfu/g. After 7 days of storage, the probiotics that survived were 2.53 × 10¹¹ colony-forming unit/g (cfu/g) and, after 30 days 1.7 × 107cfu/g. The probiotics stored for 7 days at 4 °C were tested for achieving the survival rate of the probiotics in the simulated intestinal juice for 1 hour and it was determined a survival rate of 108 cfu/g. The simulation for 4 hours in the intestinal juice reported a survival of 106cfu / g. In conclusion, starch is an effective material for encapsulating Lactobacillus cultures.
first_indexed 2024-12-13T17:12:36Z
format Article
id doaj.art-bef5aef513af495eb8b89d18d8436934
institution Directory Open Access Journal
issn 2068-6609
2559-6381
language English
last_indexed 2024-12-13T17:12:36Z
publishDate 2020-06-01
publisher Stefan cel Mare University of Suceava
record_format Article
series Food and Environment Safety
spelling doaj.art-bef5aef513af495eb8b89d18d84369342022-12-21T23:37:30ZengStefan cel Mare University of SuceavaFood and Environment Safety2068-66092559-63812020-06-01192139147THE EFFECT OF MICROENCAPSULATION AND POTATO STARCH ON THE SURVIVAL OF LACTOBACILLUS STRAINSLiliana LUCA0Mircea OROIAN1Faculty of Food Engineering, Stefan cel Mare University of Suceava, RomaniaFaculty of Food Engineering, Stefan cel Mare University of Suceava, RomaniaThe effectiveness of encapsulating ofactive bacteria in releasing probiotics depends on cell survival. In this study, a prebiotic - potato starch was used to evaluate its influence on the viability of probiotics microcapsulated. Lactobacilluscasei, Lactobacillus rhamnosus and Lactobacillus plantarumwas cultured for 16 hours and after that, the cells were added to an emulsion with 1.5% sodium alginate, supplemented with 1% prebiotics and encapsulated. Initial tests revealed that the initial culture concentration is not relevant for prebiotic supplementation. The testing of fresh microcapsules recorded a load of 3.31 × 10 ¹³ cfu/g. After 7 days of storage, the probiotics that survived were 2.53 × 10¹¹ colony-forming unit/g (cfu/g) and, after 30 days 1.7 × 107cfu/g. The probiotics stored for 7 days at 4 °C were tested for achieving the survival rate of the probiotics in the simulated intestinal juice for 1 hour and it was determined a survival rate of 108 cfu/g. The simulation for 4 hours in the intestinal juice reported a survival of 106cfu / g. In conclusion, starch is an effective material for encapsulating Lactobacillus cultures.http://fia-old.usv.ro/fiajournal/index.php/FENS/article/view/719/647probioticsprebioticslactobacillus rhamnosusl.plantaruml.casei
spellingShingle Liliana LUCA
Mircea OROIAN
THE EFFECT OF MICROENCAPSULATION AND POTATO STARCH ON THE SURVIVAL OF LACTOBACILLUS STRAINS
Food and Environment Safety
probiotics
prebiotics
lactobacillus rhamnosus
l.plantarum
l.casei
title THE EFFECT OF MICROENCAPSULATION AND POTATO STARCH ON THE SURVIVAL OF LACTOBACILLUS STRAINS
title_full THE EFFECT OF MICROENCAPSULATION AND POTATO STARCH ON THE SURVIVAL OF LACTOBACILLUS STRAINS
title_fullStr THE EFFECT OF MICROENCAPSULATION AND POTATO STARCH ON THE SURVIVAL OF LACTOBACILLUS STRAINS
title_full_unstemmed THE EFFECT OF MICROENCAPSULATION AND POTATO STARCH ON THE SURVIVAL OF LACTOBACILLUS STRAINS
title_short THE EFFECT OF MICROENCAPSULATION AND POTATO STARCH ON THE SURVIVAL OF LACTOBACILLUS STRAINS
title_sort effect of microencapsulation and potato starch on the survival of lactobacillus strains
topic probiotics
prebiotics
lactobacillus rhamnosus
l.plantarum
l.casei
url http://fia-old.usv.ro/fiajournal/index.php/FENS/article/view/719/647
work_keys_str_mv AT lilianaluca theeffectofmicroencapsulationandpotatostarchonthesurvivaloflactobacillusstrains
AT mirceaoroian theeffectofmicroencapsulationandpotatostarchonthesurvivaloflactobacillusstrains
AT lilianaluca effectofmicroencapsulationandpotatostarchonthesurvivaloflactobacillusstrains
AT mirceaoroian effectofmicroencapsulationandpotatostarchonthesurvivaloflactobacillusstrains